Abstract
Under low iron availability,plants induce the expression of various genes for iron uptake and translocation. Function of the rice ubiquitin ligases OsHRZ1 and OsHRZ2 causes overall repression of these iron-related genes at the transcript level,but their protein-level regulation is unclear. We conducted a proteome analysis to identify key regulators whose abundance was regulated by OsHRZs at the protein level. In response to iron deficiency or OsHRZ knockdown,many genes showed differential regulation between the transcript and protein levels,including the TGA-type basic leucine zipper transcription factor OsbZIP83. We also identified two glutaredoxins,OsGRX6 and OsGRX9, as OsHRZ-interacting proteins in yeast and plant cells. OsGRX6 also interacted with OsbZIP83. In vitro degradation assay suggested that OsbZIP83,OsGRX6 and OsGRX9 proteins are subjected to 26S proteasome- and OsHRZ-dependent degradation. Proteome analysis and in vitro degradation assay also suggested that OsbZIP83 protein was preferentially degraded under iron-deficient conditions in rice roots. Transgenic rice lines overexpressing OsGRX9 and OsbZIP83 showed improved tolerance to iron deficiency. Expression of iron-related genes was affected in the OsGRX9 and OsGRX6 knockdown lines,suggesting disturbed iron utilization and signaling. OsbZIP83 overexpression lines showed enhanced expression of OsYSL2 and OsNAS3,which are involved in internal iron translocation, in addition to OsGRX9 and genes related to phytoalexin biosynthesis and the salicylic acid pathway. The results suggest that OsbZIP83,OsGRX6,and OsGRX9 facilitate iron utilization downstream of the OsHRZ pathway.
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Claims 15
Claim · model-written summary
Iron deficiency or OsHRZ knockdown produced differential regulation between transcript and protein levels for many genes, including OsbZIP83.
- Taxon
- rice
Oryza sativat0 - Treatment
- iron deficiency or OsHRZ knockdowne0
- Gene
- OsbZIP83e0
- Outcome
- differential regulation between the transcript and protein levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“In response to iron deficiency or OsHRZ knockdown,many genes showed differential regulation between the transcript and protein levels,including the TGA-type basic leucine zipper transcription factor OsbZIP83”
Claim · model-written summary
OsGRX6 interacted with OsHRZ proteins in yeast and plant cells.
- Taxon
- riceOryza sativat0
- Gene
- OsGRX6e0
- Outcome
- OsHRZ-interacting proteinse0
- Setting
- yeast and plant cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“two glutaredoxins,OsGRX6 and OsGRX9, as OsHRZ-interacting proteins in yeast and plant cells”
Claim · model-written summary
OsGRX9 interacted with OsHRZ proteins in yeast and plant cells.
- Taxon
- riceOryza sativat0
- Gene
- OsGRX9e0
- Outcome
- OsHRZ-interacting proteinse0
- Setting
- yeast and plant cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“two glutaredoxins,OsGRX6 and OsGRX9, as OsHRZ-interacting proteins in yeast and plant cells”
Claim · model-written summary
OsGRX6 interacted with OsbZIP83.
- Taxon
- riceOryza sativat0
- Gene
- OsGRX6e0
- Outcome
- interacted with OsbZIP83e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“OsGRX6 also interacted with OsbZIP83”
Claim · model-written summary
In vitro assays suggested OsbZIP83 undergoes 26S proteasome- and OsHRZ-dependent degradation.
- Taxon
- riceOryza sativat0
- Gene
- OsbZIP83e0
- Outcome
- 26S proteasome- and OsHRZ-dependent degradatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“OsbZIP83,OsGRX6 and OsGRX9 proteins are subjected to 26S proteasome- and OsHRZ-dependent degradation”
Claim · model-written summary
In vitro assays suggested OsGRX6 undergoes 26S proteasome- and OsHRZ-dependent degradation.
- Taxon
- riceOryza sativat0
- Gene
- OsGRX6e0
- Outcome
- 26S proteasome- and OsHRZ-dependent degradatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“OsbZIP83,OsGRX6 and OsGRX9 proteins are subjected to 26S proteasome- and OsHRZ-dependent degradation”
Claim · model-written summary
In vitro assays suggested OsGRX9 undergoes 26S proteasome- and OsHRZ-dependent degradation.
- Taxon
- riceOryza sativat0
- Gene
- OsGRX9e0
- Outcome
- 26S proteasome- and OsHRZ-dependent degradatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“OsbZIP83,OsGRX6 and OsGRX9 proteins are subjected to 26S proteasome- and OsHRZ-dependent degradation”
Claim · model-written summary
OsbZIP83 protein was preferentially degraded in iron-deficient rice roots.
- Taxon
- riceOryza sativae0
- Organ / stage
- rootse0
- Gene
- OsbZIP83e0
- Outcome
- protein was preferentially degradede0
- Setting
- iron-deficient conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsbZIP83 protein was preferentially degraded under iron-deficient conditions in rice roots”
Claim · model-written summary
OsGRX9 overexpression improved rice tolerance to iron deficiency.
- Taxon
- riceOryza sativae0
- Treatment
- overexpressing OsGRX9e0
- Gene
- OsGRX9e0
- Outcome
- tolerance to iron deficiencye0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic rice lines overexpressing OsGRX9 and OsbZIP83 showed improved tolerance to iron deficiency”
Claim · model-written summary
OsbZIP83 overexpression improved rice tolerance to iron deficiency.
- Taxon
- riceOryza sativae0
- Treatment
- overexpressing OsGRX9 and OsbZIP83e0
- Gene
- OsbZIP83e0
- Outcome
- tolerance to iron deficiencye0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic rice lines overexpressing OsGRX9 and OsbZIP83 showed improved tolerance to iron deficiency”
Claim · model-written summary
OsGRX9 knockdown affected expression of iron-related genes.
- Taxon
- riceOryza sativat0
- Material
- OsGRX9 and OsGRX6 knockdown linese0
- Gene
- OsGRX9e0
- Outcome
- Expression of iron-related genese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Expression of iron-related genes was affected in the OsGRX9 and OsGRX6 knockdown lines”
Claim · model-written summary
OsGRX6 knockdown affected expression of iron-related genes.
- Taxon
- riceOryza sativat0
- Material
- OsGRX9 and OsGRX6 knockdown linese0
- Gene
- OsGRX6e0
- Outcome
- Expression of iron-related genese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Expression of iron-related genes was affected in the OsGRX9 and OsGRX6 knockdown lines”
Claim · model-written summary
OsbZIP83 overexpression enhanced expression of OsYSL2 and OsNAS3.
- Taxon
- riceOryza sativat0
- Treatment
- OsbZIP83 overexpressione0
- Gene
- OsYSL2 and OsNAS3e0
- Outcome
- expression of OsYSL2 and OsNAS3e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“OsbZIP83 overexpression lines showed enhanced expression of OsYSL2 and OsNAS3”
Claim · model-written summary
OsbZIP83 overexpression enhanced expression of OsGRX9 and genes related to phytoalexin biosynthesis and the salicylic acid pathway.
- Taxon
- riceOryza sativat0
- Treatment
- OsbZIP83 overexpressione0
- Gene
- OsGRX9e0
- Outcome
- OsGRX9 and genes related to phytoalexin biosynthesis and the salicylic acid pathwaye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“OsbZIP83 overexpression lines showed enhanced expression of OsYSL2 and OsNAS3,which are involved in internal iron translocation, in addition to OsGRX9 and genes related to phytoalexin biosynthesis and the salicylic acid pathway”
Claim · model-written summary
The authors infer that OsbZIP83, OsGRX6 and OsGRX9 facilitate iron utilization downstream of OsHRZ.
- Taxon
- riceOryza sativat0
- Gene
- OsbZIP83,OsGRX6,and OsGRX9e0
- Outcome
- iron utilizatione0
- Qualifier
- downstream of the OsHRZ pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“OsbZIP83,OsGRX6,and OsGRX9 facilitate iron utilization downstream of the OsHRZ pathway”