Abstract
We investigated the effect of tissue source selection in combination with ion beam irradiation on generating flower-color mutants in Chrysanthemum morifolium. Flower color mutation in the chrysanthemum cultivar YoMystery (purple flowers) was used as an index to estimate mutation frequency and the spectrum of mutations. Somaclones regenerated from petal and leaf tissues and ion-beam irradiated clones derived from the two tissues were analyzed for stem length and flower color mutation. Three Gy of argon ion beams was regarded as an appropriate dose for irradiation because the resulting color mutants maintained an adequate stem length suitable for commercial use even with high mutation frequencies. Statistical analysis of 7,258 individuals from three separate experiments revealed that (1) somaclones from petal tissue had a higher mutation frequency (2.91 %, r = 0.55) than leaf somaclones (2.01 %, r = 0.26); (2) the effect of tissue source on the frequency of flower color mutations was significantly enhanced when combined with ion-beam irradiation in that the mutation frequency of ion-beam irradiated petal clones was 6.47 % (r = 0.77), whereas that of ion-beam irradiated leaf clones was 3.89 % (r = 0.52); and (3) the spectrum of carotenoid color mutations was remarkably increased by ion-beam irradiation in that both leaf and petal ion-beam irradiated clones had brown, red and yellow flowers in contrast to the soma clones that only produced yellow flowers. These results suggest that intentional or directed induction of flower color mutants with practical value is possible by combining tissue source selection with ion beam irradiation.
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Claims 5
Claim · model-written summary
Three Gy argon ion beams gave high mutation frequency while maintaining commercially adequate stem length.
Verbatim
e0“Three Gy of argon ion beams was regarded as an appropriate dose for irradiation because the resulting color mutants maintained an adequate stem length suitable for commercial use even with high mutation frequencies.”
Claim · model-written summary
Petal somaclones had a higher flower-color mutation frequency than leaf somaclones (2.91% versus 2.01%).
- Taxon
- chrysanthemum
.jpeg?width=60)
Chrysanthemumt0 - Material
- somaclonese0
- Organ / stage
- petal tissuee0
- Treatment
- petal tissuee0
- Outcome
- mutation frequencye0
- Direction
- highere0
- Value
- 2.91 %e0
- Qualifier
- 2.01 %e0
- Comparator
- leaf somaclonese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpeg?width=500)
Verbatim
e0“Statistical analysis of 7,258 individuals from three separate experiments revealed that (1) somaclones from petal tissue had a higher mutation frequency (2.91 %, r = 0.55) than leaf somaclones (2.01 %, r = 0.26); (2) the effect of tissue source on the frequency of flower color mutations was significantly enhanced when combined with ion-beam irradiation in that the mutation frequency of ion-beam irradiated petal clones was 6.47 % (r = 0.77), whereas that of ion-beam irradiated leaf clones was 3.89 % (r = 0.52); and (3) the spectrum of carotenoid color mutations was remarkably increased by ion-beam irradiation in that both leaf and petal ion-beam irradiated clones had brown, red and yellow flowers in contrast to the soma clones that only produced yellow flowers.”
t0“chrysanthemum”
Claim · model-written summary
Ion irradiated petal clones had a 6.47% mutation frequency versus 3.89% in ion irradiated leaf clones.
- Taxon
- chrysanthemumChrysanthemumt0
- Organ / stage
- petale0
- Treatment
- ion-beam irradiatione0
- Outcome
- mutation frequencye0
- Direction
- enhancede0
- Value
- 6.47 %e0
- Qualifier
- 3.89 %e0
- Comparator
- ion-beam irradiated leaf clonese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Statistical analysis of 7,258 individuals from three separate experiments revealed that (1) somaclones from petal tissue had a higher mutation frequency (2.91 %, r = 0.55) than leaf somaclones (2.01 %, r = 0.26); (2) the effect of tissue source on the frequency of flower color mutations was significantly enhanced when combined with ion-beam irradiation in that the mutation frequency of ion-beam irradiated petal clones was 6.47 % (r = 0.77), whereas that of ion-beam irradiated leaf clones was 3.89 % (r = 0.52); and (3) the spectrum of carotenoid color mutations was remarkably increased by ion-beam irradiation in that both leaf and petal ion-beam irradiated clones had brown, red and yellow flowers in contrast to the soma clones that only produced yellow flowers.”
t0“chrysanthemum”
Claim · model-written summary
Ion irradiation increased the spectrum of carotenoid flower colors in petal and leaf clones.
- Taxon
- chrysanthemumChrysanthemumt0
- Treatment
- ion-beam irradiatione0
- Outcome
- spectrum of carotenoid color mutationse0
- Direction
- increasede0
- Qualifier
- brown, red and yellow flowerse0
- Comparator
- soma clonese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Statistical analysis of 7,258 individuals from three separate experiments revealed that (1) somaclones from petal tissue had a higher mutation frequency (2.91 %, r = 0.55) than leaf somaclones (2.01 %, r = 0.26); (2) the effect of tissue source on the frequency of flower color mutations was significantly enhanced when combined with ion-beam irradiation in that the mutation frequency of ion-beam irradiated petal clones was 6.47 % (r = 0.77), whereas that of ion-beam irradiated leaf clones was 3.89 % (r = 0.52); and (3) the spectrum of carotenoid color mutations was remarkably increased by ion-beam irradiation in that both leaf and petal ion-beam irradiated clones had brown, red and yellow flowers in contrast to the soma clones that only produced yellow flowers.”
t0“chrysanthemum”
Claim · model-written summary
Combining tissue-source selection and ion irradiation may permit directed production of commercially useful flower-color mutants.
Verbatim
e0“These results suggest that intentional or directed induction of flower color mutants with practical value is possible by combining tissue source selection with ion beam irradiation.”
The same questions in other papers
radiation · physiology: pigments · ChrysanthemumPositive and negative results
1 in the opposite direction
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.