Abstract
This study explores the root-associated microbiome of Calotropis procera, a drought-adapted, invasive plant thriving in Brazil. We analyzed microbial communities from the root endosphere, rhizosphere, and adjacent soil in two contrasting ecosystems: Caatinga (semi-arid) and Restinga (coastal). Using 16S rDNA sequencing and shotgun metagenomics, we tested three hypotheses: (I) environmental specificity of the rhizospheric bacterial microbiome, (II) continuity of bacterial composition between bulk soil and rhizosphere, and (III) host-driven filtering of the endophytic microbiome. Despite differing soil conditions – more sodium in Restinga and higher organic carbon in Caatinga – microbial profiles in root compartments remained consistent. The root endosphere was enriched with stress-tolerant bacteria and novel archaea, while fungal genera included Fusarium and Puccinia. Results partially supported environmental specificity and showed moderate soil-rhizosphere continuity, with evidence of plant-mediated selection. Host filtering was evident for bacteria and fungi but not archaea. These data indicate a C. procera-mediated regulation of its root microbiome composition, whereby the plant may either selectively recruit specific taxa from prevalent soil microbial communities (e.g., through root exudates) or vertically transmit a conserved subset of its microbiome via seeds. Our study enhances understanding of the C. procera microbiome and its microbial interactions, identifying potential candidates for future biotechnological applications.
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Claims 8
Claim · model-written summary
Root microbial profiles remained consistent despite different soil conditions.
- Taxon
- Calotropis procera
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Calotropis procerae1 - Outcome
- microbial profiles in root compartmentse0
- Direction
- remained consistente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“microbial profiles in root compartments remained consistent”
e1“Calotropis procera”
Claim · model-written summary
Root endosphere was enriched with stress tolerant bacteria and novel archaea.
- Taxon
- Calotropis proceraCalotropis procerae1
- Outcome
- stress-tolerant bacteria and novel archaeae0
- Direction
- enrichede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The root endosphere was enriched with stress-tolerant bacteria and novel archaea”
e1“Calotropis procera”
Claim · model-written summary
Fungal genera included Fusarium and Puccinia.
- Taxon
- Calotropis proceraCalotropis procerae1
- Outcome
- fungal generae0
Fusarium
PucciniaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fungal genera included Fusarium and Puccinia”
e1“Calotropis procera”
Claim · model-written summary
Results partially supported environmental specificity.
- Taxon
- Calotropis proceraCalotropis procerae1
- Outcome
- environmental specificitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results partially supported environmental specificity”
e1“Calotropis procera”
Claim · model-written summary
Soil and rhizosphere showed moderate continuity.
- Taxon
- Calotropis proceraCalotropis procerae1
- Outcome
- soil-rhizosphere continuitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“showed moderate soil-rhizosphere continuity”
e1“Calotropis procera”
Claim · model-written summary
Host filtering was evident for bacteria and fungi.
- Taxon
- Calotropis proceraCalotropis procerae1
- Outcome
- Host filteringe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Host filtering was evident for bacteria and fungi but not archaea.”
e1“Calotropis procera”
Claim · model-written summary
Host filtering was not evident for archaea.
- Taxon
- Calotropis proceraCalotropis procerae1
- Outcome
- not archaeae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Host filtering was evident for bacteria and fungi but not archaea.”
e1“Calotropis procera”
Claim · model-written summary
The host appears to regulate root microbiome composition.
- Taxon
- Calotropis proceraCalotropis procerae1
- Outcome
- root microbiome compositione0
- Qualifier
- C. procera-mediated regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“C. procera-mediated regulation of its root microbiome composition”
e1“Calotropis procera”