Abstract
Jasmonate (JA) accumulation and signaling play key roles in regulating plant growth and flower development. The optimal timing of flowering is critical for the quality and yield of Brassica juncea. B-box (BBX) factors play roles in plant floral transition and stress response processes. However, whether BBX6-2 responds to JA signaling to regulate flowering time in B. juncea remains unclear. Here, we characterized the biological function of BjuBBX6-2 in response to JA in regulating the flowering time of B. juncea. Subcellular localization and transcriptional activation activity assays showed that BjuBBX6-2 localizes in the nucleus and exhibits transcriptional activation activity. Spraying BjuBBX6-2-overexpressing or BjuBBX6-2-silenced B. juncea plants with 50 μmol/L methyl JA significantly accelerated or delayed their flowering time, respectively. We demonstrated that BjuBBX6-2 interacts with the flowering factor Nuclear Factor Y, Subunit C4 (BjuNF-YC4), which interacts with Nuclear Factor Y, Subunit B2/3 (BjuNF-YB2/3). The BjuBBX6-2-BjuNF-YC4-BjuNF-YB2/3 multiple-protein complex bound to the promoter of the downstream flowering integrator gene SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (BjuSOC1) and promoted its expression. JASMONATE ZIM-DOMAIN 1 (BjuJAZ1), a key factor in the JA signaling pathway, interacted with BjuBBX6-2 to inhibit the activation of BjuSOC1 by BjuBBX6-2. In summary, BjuBBX6-2 cooperates with BjuNF-YC4 and BjuJAZ1 in response to JA signaling to participate in the flowering regulation of B. juncea. These findings highlight a previously uncharacterized mechanism of JA signaling-mediated flowering time regulation via interactions between BjuBBX6-2 and the integrator gene BjuSOC1, providing prospects for breeding enhanced B. juncea cultivars.
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Claims 7
Claim · model-written summary
BjuBBX6-2 localizes in the nucleus and shows transcriptional activation activity in Brassica juncea.
- Taxon
- Brassica juncea
Brassica junceat0 - Gene
- BjuBBX6-2e0
- Outcome
- localizes in the nucleus and exhibits transcriptional activation activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica juncea”
e0“BjuBBX6-2 localizes in the nucleus and exhibits transcriptional activation activity”
Claim · model-written summary
Spraying BjuBBX6-2-overexpressing B. juncea plants with 50 μmol/L methyl JA significantly accelerated flowering time.
- Taxon
- Brassica junceaBrassica junceat0
- Material
- BjuBBX6-2-overexpressinge0
- Treatment
- 50 μmol/L methyl JAe0
- Outcome
- flowering timee0
- Direction
- acceleratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica juncea”
e0“Spraying BjuBBX6-2-overexpressing or BjuBBX6-2-silenced B. juncea plants with 50 μmol/L methyl JA significantly accelerated or delayed their flowering time, respectively”
Claim · model-written summary
Spraying BjuBBX6-2-silenced B. juncea plants with 50 μmol/L methyl JA significantly delayed flowering time.
- Taxon
- Brassica junceaBrassica junceat0
- Material
- BjuBBX6-2-silencede0
- Treatment
- 50 μmol/L methyl JAe0
- Outcome
- flowering timee0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica juncea”
e0“Spraying BjuBBX6-2-overexpressing or BjuBBX6-2-silenced B. juncea plants with 50 μmol/L methyl JA significantly accelerated or delayed their flowering time, respectively”
Claim · model-written summary
BjuBBX6-2 interacts with the flowering factor BjuNF-YC4 in B. juncea.
- Taxon
- Brassica junceaBrassica junceat0
- Gene
- BjuBBX6-2e0
- Outcome
- interacts with the flowering factor Nuclear Factor Y, Subunit C4 (BjuNF-YC4)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica juncea”
e0“BjuBBX6-2 interacts with the flowering factor Nuclear Factor Y, Subunit C4 (BjuNF-YC4)”
Claim · model-written summary
BjuNF-YC4 interacts with BjuNF-YB2/3 in B. juncea.
- Taxon
- Brassica junceaBrassica junceat0
- Gene
- Nuclear Factor Y, Subunit B2/3 (BjuNF-YB2/3)e0
- Outcome
- interacts with Nuclear Factor Y, Subunit B2/3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica juncea”
e0“which interacts with Nuclear Factor Y, Subunit B2/3 (BjuNF-YB2/3)”
Claim · model-written summary
The BjuBBX6-2-BjuNF-YC4-BjuNF-YB2/3 protein complex bound the promoter of the flowering integrator gene BjuSOC1 and promoted its expression.
- Taxon
- Brassica junceaBrassica junceat0
- Gene
- BjuSOC1e0
- Outcome
- bound to the promoter of the downstream flowering integrator gene SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (BjuSOC1) and promoted its expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica juncea”
e0“The BjuBBX6-2-BjuNF-YC4-BjuNF-YB2/3 multiple-protein complex bound to the promoter of the downstream flowering integrator gene SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (BjuSOC1) and promoted its expression”
Claim · model-written summary
BjuJAZ1, a key factor of JA signaling, interacted with BjuBBX6-2 and thereby inhibited the activation of BjuSOC1 by BjuBBX6-2.
- Taxon
- Brassica junceaBrassica junceat0
- Gene
- BjuJAZ1e0
- Outcome
- inhibit the activation of BjuSOC1 by BjuBBX6-2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica juncea”
e0“JASMONATE ZIM-DOMAIN 1 (BjuJAZ1), a key factor in the JA signaling pathway, interacted with BjuBBX6-2 to inhibit the activation of BjuSOC1 by BjuBBX6-2”