Abstract
Meristem activity is controlled by the CLAVATA (CLV) signaling pathway, which involves a suite of leucine-rich receptor (LRR) receptors, receptor-like proteins, and CLV-EMBRYO SURROUNDING REGION (CLE) peptide ligands. FASCIATED EAR 3 (FEA3) is a leucine-rich receptor (LRR) receptor-like protein important for meristem maintenance in maize and acts independently of canonical CLV receptors. To identify FEA3's interaction network, we used TurboID-based proximity labeling in Zea mays meristems and identified a putative co-receptor, BARELY ANY MERISTEM 1D (BAM1D). BAM1D and FEA3 proximity labeling proteomes shared over 40 proteins, including many signaling proteins, suggesting they feed into a common signaling pathway. fea3 was epistatic to bam1d in the control of inflorescence meristem (IM) size, supporting the idea that FEA3 and BAM1D interact physically. However, fea3 and bam1d act antagonistically because fea3 mutants had larger IMs, whereas bam1d mutants produced smaller IMs. This study demonstrates how in vivo TurboID-based proximity labeling clarifies complex genetic interactions between CLV receptors and expands our knowledge of downstream signaling components of CLV signaling pathways, which are largely uncharacterized. Our findings support the notion that multiple, partially overlapping CLV receptor complexes coordinately control meristem maintenance.
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Claims 7
Claim · model-written summary
TurboID proximity labeling in maize meristems identified BAM1D as a putative co-receptor of FEA3.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we used TurboID-based proximity labeling in Zea mays meristems and identified a putative co-receptor, BARELY ANY MERISTEM 1D (BAM1D)”
Claim · model-written summary
BAM1D and FEA3 proximity labeling proteomes shared over 40 proteins, including many signaling proteins.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize ear”
e0“BAM1D and FEA3 proximity labeling proteomes shared over 40 proteins”
Claim · model-written summary
fea3 was epistatic to bam1d in the control of inflorescence meristem size.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize ear”
e0“fea3 was epistatic to bam1d in the control of inflorescence meristem (IM) size”
Claim · model-written summary
fea3 mutants had larger inflorescence meristems.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize ear”
e0“fea3 mutants had larger IMs”
Claim · model-written summary
bam1d mutants produced smaller inflorescence meristems.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize ear”
e0“bam1d mutants produced smaller IMs”
Claim · model-written summary
fea3 and bam1d act antagonistically.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize ear”
e0“fea3 and bam1d act antagonistically”
Claim · model-written summary
The authors propose that multiple partially overlapping CLV receptor complexes coordinately control meristem maintenance.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize ear”
e0“multiple, partially overlapping CLV receptor complexes coordinately control meristem maintenance”