Abstract
The expansin-like subfamilies (EXLA and EXLB) are vital for plant cell wall dynamics, but it remains uncharacterized in wild tetraploid and hexaploid Ipomoea batatas, and its role in the storage root (SR) development is poorly understood. In this work, we identified 4, 3, 3, and 3 EXLAs, alongside 11, 9, 13, and 8 EXLBs, in diploid I. trifida strain Y22, wild tetraploid I. batatas strain Y428B, and hexaploid I. batatas strain Y601, and cultivated sweet potato ‘Nancy Hall’, respectively. A comprehensive bioinformatic analysis of the expansin-like genes and proteins was performed to reveal their potential roles in SR development. Gene expression profiling showed that EXLA members were expressed during SR development, while approximately half of the EXLB members were expressed in Y22, Y428B (pencil root), Y601, and NH, respectively. Proteomic analysis (4D-DIA) detected 2, 1, 1, and 1 EXLAs, and 3, 3, 3, and 3 EXLBs in the mature SRs of the respective species. Integrated transcriptomic and proteomic analyses suggested that downregulating Iba6xEXLB2 and Iba6xEXLB1 may be associated with SR swelling in sweet potato. Furthermore, subcellular localization assays confirmed that Iba6xEXLB2 and Iba6xEXLB8 are localized to the cell wall/membrane. This study enhances the understanding of the expansin-like gene subfamily in sweet potato and its wild relatives and lays the groundwork for future functional studies on the role of expansin-like genes in SR development.
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Claims 8
Claim · model-written summary
Four, three, three and three EXLA genes were identified in the diploid I. trifida, wild tetraploid, hexaploid and cultivated sweet potato genotypes respectively.
- Taxon
- Sweet Potatoes
_Flower.jpg?width=60)
Ipomoea batatast0 - Material
- diploid I. trifida strain Y22, wild tetraploid I. batatas strain Y428B, and hexaploid I. batatas strain Y601, and cultivated sweet potatoe0
- Outcome
- EXLAse0
- Value
- 4, 3, 3, and 3 EXLAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Flower.jpg?width=500)
Verbatim
t0“Sweet Potatoes”
e0“we identified 4, 3, 3, and 3 EXLAs, alongside 11, 9, 13, and 8 EXLBs, in diploid I. trifida strain Y22, wild tetraploid I. batatas strain Y428B, and hexaploid I. batatas strain Y601, and cultivated sweet potato”
Claim · model-written summary
Eleven, nine, thirteen and eight EXLB genes were identified in the same four genotypes respectively.
- Taxon
- Sweet PotatoesIpomoea batatast0
- Material
- diploid I. trifida strain Y22, wild tetraploid I. batatas strain Y428B, and hexaploid I. batatas strain Y601, and cultivated sweet potatoe0
- Outcome
- EXLBse0
- Value
- 11, 9, 13, and 8 EXLBse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potatoes”
e0“we identified 4, 3, 3, and 3 EXLAs, alongside 11, 9, 13, and 8 EXLBs, in diploid I. trifida strain Y22, wild tetraploid I. batatas strain Y428B, and hexaploid I. batatas strain Y601, and cultivated sweet potato”
Claim · model-written summary
EXLA members were expressed during storage root development.
- Taxon
- Sweet PotatoesIpomoea batatast0
- Organ / stage
- SR developmente0
- Gene
- EXLAe0
- Outcome
- expressed during SR developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potatoes”
e0“EXLA members were expressed during SR development”
Claim · model-written summary
Approximately half of the EXLB members were expressed in Y22, Y428B, Y601 and NH.
- Taxon
- Sweet PotatoesIpomoea batatast0
- Material
- Y22, Y428B (pencil root), Y601, and NHe0
- Gene
- EXLBe0
- Outcome
- expressede0
- Value
- approximately halfe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potatoes”
e0“approximately half of the EXLB members were expressed in Y22, Y428B (pencil root), Y601, and NH”
Claim · model-written summary
Proteomic analysis detected 2, 1, 1 and 1 EXLAs in the mature storage roots of the respective species.
- Taxon
- Sweet PotatoesIpomoea batatast0
- Organ / stage
- mature SRse0
- Outcome
- EXLAse0
- Value
- 2, 1, 1, and 1 EXLAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potatoes”
e0“Proteomic analysis (4D-DIA) detected 2, 1, 1, and 1 EXLAs, and 3, 3, 3, and 3 EXLBs in the mature SRs of the respective species”
Claim · model-written summary
Proteomic analysis detected 3, 3, 3 and 3 EXLBs in the mature storage roots of the respective species.
- Taxon
- Sweet PotatoesIpomoea batatast0
- Organ / stage
- mature SRse0
- Outcome
- EXLBse0
- Value
- 3, 3, 3, and 3 EXLBse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potatoes”
e0“Proteomic analysis (4D-DIA) detected 2, 1, 1, and 1 EXLAs, and 3, 3, 3, and 3 EXLBs in the mature SRs of the respective species”
Claim · model-written summary
Downregulation of Iba6xEXLB2 and Iba6xEXLB1 may be associated with storage root swelling in sweet potato.
- Taxon
- sweet potatoIpomoea batatase0
- Gene
- Iba6xEXLB2 and Iba6xEXLB1e0
- Outcome
- SR swellinge0
- Direction
- downregulatinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Integrated transcriptomic and proteomic analyses suggested that downregulating Iba6xEXLB2 and Iba6xEXLB1 may be associated with SR swelling in sweet potato”
Claim · model-written summary
Iba6xEXLB2 and Iba6xEXLB8 are localized to the cell wall/membrane.
- Taxon
- Sweet PotatoesIpomoea batatast0
- Gene
- Iba6xEXLB2 and Iba6xEXLB8e0
- Outcome
- localized to the cell wall/membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potatoes”
e0“subcellular localization assays confirmed that Iba6xEXLB2 and Iba6xEXLB8 are localized to the cell wall/membrane”