Paper · 2026 · Weed Research

Suppression of Trifolium repens in Poa pratensis Using Sequential Application of Systemic and Contact Herbicides

Masood Ghasemi Ghehsareh; Seyedeh Maryam Mesbahi; Somayeh Esmaeili; et al.

6
claims
6
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

ABSTRACT Trifolium repens L. (white clover) is a common perennial weed that aggressively colonises turfgrass, disrupting its uniformity and aesthetic quality. Effective management of T. repens is essential for preserving the visual and functional integrity of turfgrass systems. This study evaluated the efficacy of a sequential herbicide application strategy for controlling T. repens in Poa pratensis L. (Kentucky bluegrass). A randomised complete block design with three replications and nine treatments was employed, comprising systemic herbicides—U46‐Combi Fluid (U) (containing 2,4‐D and MCPA) at 1.5 and 2 L ha −1 (1012.5 and 1350 g ai ha −1 ), and Duplosan Super (D) (containing MCPA, Dichlorprop‐P, and Mecoprop‐P) at 2 and 2.5 L ha −1 (1200 and 1500 g ai ha −1 )—followed by a contact herbicide, Ioxynil (I), at 0 or 2 L ha −1 (0 and 4500 g ai ha −1 ). Results showed that the D2.5 + I2 and D2 + I2 treatments achieved 100% weed control efficiency and complete suppression of T. repens regrowth, significantly reducing its biomass while enhancing P. pratensis visual quality, chlorophyll content, and biomass. Correlation and principal component analyses highlighted strong negative associations between weed ( T. repens ) and turfgrass ( P. pratensis ) metrics. These high‐performing sequential treatments represent an effective and practical approach to T. repens management. This approach's key advantage lies in preventing weed regrowth, which can reduce the need for repeated applications and thereby lower the overall herbicide load in turfgrass systems. Further research should investigate the long‐term ecological and economic implications of this method, as well as its adaptability across various turfgrass species and environmental conditions.

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Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sequential D2.5 + I2 and D2 + I2 herbicide treatments achieved 100% weed control efficiency against T. repens in Poa pratensis turf.

Taxon
T. repensTrifolium repense0
Treatment
D2.5 + I2 and D2 + I2 treatmentse0
Outcome
weed control efficiencye0
Direction
increaseinferrede0
Value
100%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium repens
Trifolium repens · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the D2.5 + I2 and D2 + I2 treatments achieved 100% weed control efficiency and complete suppression of T. repens regrowth”

✓ located verbatim in the source · abstract, characters 883–1004 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The D2.5 + I2 and D2 + I2 treatments completely suppressed regrowth of T. repens.

Taxon
T. repensTrifolium repense0
Treatment
D2.5 + I2 and D2 + I2 treatmentse0
Outcome
T. repens regrowthe0
Direction
complete suppressione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium repens
Trifolium repens · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the D2.5 + I2 and D2 + I2 treatments achieved 100% weed control efficiency and complete suppression of T. repens regrowth”

✓ located verbatim in the source · abstract, characters 883–1004 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The D2.5 + I2 and D2 + I2 treatments significantly reduced T. repens biomass.

Taxon
T. repensTrifolium repense0
Treatment
D2.5 + I2 and D2 + I2 treatmentse0
Outcome
its biomasse0
Direction
reducinge0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium repens
Trifolium repens · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the D2.5 + I2 and D2 + I2 treatments achieved 100% weed control efficiency and complete suppression of T. repens regrowth, significantly reducing its biomass”

✓ located verbatim in the source · abstract, characters 883–1040 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The D2.5 + I2 and D2 + I2 treatments enhanced P. pratensis visual quality, chlorophyll content and biomass.

Taxon
P. pratensisPoa pratensise0
Treatment
D2.5 + I2 and D2 + I2 treatmentse0
Outcome
visual quality, chlorophyll content, and biomasse0
Direction
enhancinge0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Poa pratensis
Poa pratensis · TaxonRasbak 08:35, 1 May 2007 (UTC) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the D2.5 + I2 and D2 + I2 treatments achieved 100% weed control efficiency and complete suppression of T. repens regrowth, significantly reducing its biomass while enhancing P. pratensis visual quality, chlorophyll content, and biomass”

✓ located verbatim in the source · abstract, characters 883–1118 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Correlation and principal component analyses showed strong negative associations between the weed T. repens and P. pratensis turfgrass metrics.

Taxon
P. pratensisPoa pratensise0
Interactor
T. repensTrifolium repense0weed
Outcome
turfgrass ( P. pratensis ) metricse0
Direction
strong negative associationse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Poa pratensis
Poa pratensis · TaxonRasbak 08:35, 1 May 2007 (UTC) · CC BY-SA 3.0 · Wikimedia Commons ↗
Trifolium repens
Trifolium repens · InteractorIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“strong negative associations between weed ( T. repens ) and turfgrass ( P. pratensis ) metrics”

✓ located verbatim in the source · abstract, characters 1177–1271 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors present the sequential treatments as an effective and practical approach to T. repens management.

Taxon
T. repensTrifolium repense0
Outcome
effective and practical approach to T. repens managemente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium repens
Trifolium repens · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“sequential treatments represent an effective and practical approach to T. repens management”

✓ located verbatim in the source · abstract, characters 1295–1386 · show in text ↑