Abstract
An efficient in vitro regeneration protocol was developed for Cordyline terminalis via indirect organogenesis using different explants and plant growth regulator (PGR) combinations. Nodal segments, shoot tips and leaf bases were cultured on Murashige and Skoog (MS) medium supplemented with varying 6-Benzylaminopurine (BAP), α-Naphthaleneacetic acid (NAA) and 2,4-Dichlorophenoxyacetic acid (2,4-D) concentrations. Among the explants, nodal segments showed the highest morphogenic response with early shoot initiation and maximum shoot production. The treatment with BAP 1.0 mg L-1 + NAA 0.5 mg L-1 (H3) recorded the highest callus induction (83.33 %), highest callus intensity (4.40) and callus index (367.00). Media supplemented with 2,4-D promoted early and dense callus formation but resulted in limited shoot regeneration. Callus tissues were subcultured at 3-week intervals onto fresh regeneration medium containing BAP (1.0 mg L-1) + NAA (0.5 mg L-1) to promote shoot differentiation. It resulted in the regeneration of multiple shoot primordia (up to 4-5 shoots per culture). Correlation analysis indicated a strong positive relationship between callus intensity and shoot proliferation efficiency. These results highlight the critical role of explant selection and hormonal balance in optimizing callus-mediated regeneration. The standardized protocol developed in this study can be effectively utilized for the large-scale propagation and conservation of Cordyline terminalis.
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Claims 11
Claim · model-written summary
Nodal segments showed the highest morphogenic response among the explants tested.
- Taxon
- Cordyline terminalis
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Cordyline fruticosae0 - Organ / stage
- nodal segmentse1
- Treatment
- different explantse0
- Outcome
- morphogenic responsee1
- Direction
- higheste1
- Comparator
- shoot tips and leaf basese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“An efficient in vitro regeneration protocol was developed for Cordyline terminalis via indirect organogenesis using different explants and plant growth regulator (PGR) combinations”
e1“Among the explants, nodal segments showed the highest morphogenic response with early shoot initiation and maximum shoot production”
e2“Nodal segments, shoot tips and leaf bases were cultured”
Claim · model-written summary
Nodal segments gave maximum shoot production among the explants tested.
- Taxon
- Cordyline terminalisCordyline fruticosae0
- Organ / stage
- nodal segmentse1
- Treatment
- different explantse0
- Outcome
- shoot productione1
- Direction
- maximume1
- Comparator
- shoot tips and leaf basese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient in vitro regeneration protocol was developed for Cordyline terminalis via indirect organogenesis using different explants and plant growth regulator (PGR) combinations”
e1“Among the explants, nodal segments showed the highest morphogenic response with early shoot initiation and maximum shoot production”
e2“Nodal segments, shoot tips and leaf bases were cultured”
Claim · model-written summary
Nodal segments gave early shoot initiation among the explants tested.
- Taxon
- Cordyline terminalisCordyline fruticosae0
- Organ / stage
- nodal segmentse1
- Treatment
- different explantse0
- Outcome
- early shoot initiatione1
- Direction
- earlye1
- Comparator
- shoot tips and leaf basese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient in vitro regeneration protocol was developed for Cordyline terminalis via indirect organogenesis using different explants and plant growth regulator (PGR) combinations”
e1“Among the explants, nodal segments showed the highest morphogenic response with early shoot initiation and maximum shoot production”
e2“Nodal segments, shoot tips and leaf bases were cultured”
Claim · model-written summary
BAP 1.0 mg L-1 + NAA 0.5 mg L-1 gave the highest callus induction (83.33 %).
- Taxon
- Cordyline terminalisCordyline fruticosae0
- Treatment
- BAP 1.0 mg L-1 + NAA 0.5 mg L-1e3
- Outcome
- callus inductione3
- Direction
- higheste3
- Value
- 83.33 %e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient in vitro regeneration protocol was developed for Cordyline terminalis via indirect organogenesis using different explants and plant growth regulator (PGR) combinations”
e3“The treatment with BAP 1.0 mg L-1 + NAA 0.5 mg L-1 (H3) recorded the highest callus induction (83.33 %), highest callus intensity (4.40) and callus index (367.00)”
Claim · model-written summary
BAP 1.0 mg L-1 + NAA 0.5 mg L-1 gave the highest callus intensity (4.40).
- Taxon
- Cordyline terminalisCordyline fruticosae0
- Treatment
- BAP 1.0 mg L-1 + NAA 0.5 mg L-1e3
- Outcome
- callus intensitye3
- Direction
- higheste3
- Value
- 4.40e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient in vitro regeneration protocol was developed for Cordyline terminalis via indirect organogenesis using different explants and plant growth regulator (PGR) combinations”
e3“The treatment with BAP 1.0 mg L-1 + NAA 0.5 mg L-1 (H3) recorded the highest callus induction (83.33 %), highest callus intensity (4.40) and callus index (367.00)”
Claim · model-written summary
BAP 1.0 mg L-1 + NAA 0.5 mg L-1 gave the highest callus index (367.00).
- Taxon
- Cordyline terminalisCordyline fruticosae0
- Treatment
- BAP 1.0 mg L-1 + NAA 0.5 mg L-1e3
- Outcome
- callus indexe3
- Direction
- higheste3
- Value
- 367.00e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient in vitro regeneration protocol was developed for Cordyline terminalis via indirect organogenesis using different explants and plant growth regulator (PGR) combinations”
e3“The treatment with BAP 1.0 mg L-1 + NAA 0.5 mg L-1 (H3) recorded the highest callus induction (83.33 %), highest callus intensity (4.40) and callus index (367.00)”
Claim · model-written summary
2,4-D promoted early and dense callus formation.
- Taxon
- Cordyline terminalisCordyline fruticosae0
- Treatment
- 2,4-De4
- Outcome
- callus formatione4
- Direction
- promotede4
- Qualifier
- early and densee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient in vitro regeneration protocol was developed for Cordyline terminalis via indirect organogenesis using different explants and plant growth regulator (PGR) combinations”
e4“Media supplemented with 2,4-D promoted early and dense callus formation but resulted in limited shoot regeneration”
Claim · model-written summary
2,4-D resulted in limited shoot regeneration.
- Taxon
- Cordyline terminalisCordyline fruticosae0
- Treatment
- 2,4-De4
- Outcome
- shoot regeneratione4
- Direction
- limitede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient in vitro regeneration protocol was developed for Cordyline terminalis via indirect organogenesis using different explants and plant growth regulator (PGR) combinations”
e4“Media supplemented with 2,4-D promoted early and dense callus formation but resulted in limited shoot regeneration”
Claim · model-written summary
Subcultured callus on BAP and NAA regenerated multiple shoot primordia, up to 4-5 shoots per culture.
- Taxon
- Cordyline terminalisCordyline fruticosae0
- Treatment
- BAP (1.0 mg L-1) + NAA (0.5 mg L-1)e6
- Outcome
- multiple shoot primordiae5
- Direction
- increaseinferrede5
- Value
- up to 4-5 shoots per culturee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient in vitro regeneration protocol was developed for Cordyline terminalis via indirect organogenesis using different explants and plant growth regulator (PGR) combinations”
e5“resulted in the regeneration of multiple shoot primordia (up to 4-5 shoots per culture)”
e6“Callus tissues were subcultured at 3-week intervals onto fresh regeneration medium containing BAP (1.0 mg L-1) + NAA (0.5 mg L-1) to promote shoot differentiation”
Claim · model-written summary
Callus intensity and shoot proliferation efficiency were strongly and positively related.
- Taxon
- Cordyline terminalisCordyline fruticosae0
- Organ / stage
- calluse7
- Outcome
- shoot proliferation efficiencye7
- Direction
- positivee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient in vitro regeneration protocol was developed for Cordyline terminalis via indirect organogenesis using different explants and plant growth regulator (PGR) combinations”
e7“Correlation analysis indicated a strong positive relationship between callus intensity and shoot proliferation efficiency”
Claim · model-written summary
The standardized protocol can be used for large-scale propagation and conservation of Cordyline terminalis.
- Taxon
- Cordyline terminalisCordyline fruticosae1
- Outcome
- large-scale propagation and conservatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient in vitro regeneration protocol was developed for Cordyline terminalis via indirect organogenesis using different explants and plant growth regulator (PGR) combinations”
e1“The standardized protocol developed in this study can be effectively utilized for the large-scale propagation and conservation of Cordyline terminalis”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.