Abstract
Accurate identification of commercially cultivated coffee species is necessary since the cup quality may be attributed to the kind of species used. Hence, DNA barcoding was performed using nuclear ribosomal internal transcribe spacer (ITS) and maturase K (matK). Both markers had 100% amplification and sequencing success rates. Although ITS had lower resolution in Coffea species, it efficiently discriminated Coffea liberica. The matK barcode discriminated all the species. Findings revealed that matK is an efficient barcode over ITS for commercially cultivated Coffea species by generating the highest rate of both universality and discrimination power. DNA barcoding as a method of authentication will benefit the coffee industry and coffee growers for large-scale plantations.
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Claims 6
Claim · model-written summary
Both ITS and matK barcode markers had 100% amplification and sequencing success in Coffea spp.
CoffeaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Molecular Authentication of Commercially Cultivated Coffee (Coffea spp.) in the Philippines using DNA Barcodes”
e1“Both markers had 100% amplification and sequencing success rates”
Claim · model-written summary
ITS had lower resolution than matK in Coffea species.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Molecular Authentication of Commercially Cultivated Coffee (Coffea spp.) in the Philippines using DNA Barcodes”
e2“Although ITS had lower resolution in Coffea species”
e5“matK is an efficient barcode over ITS for commercially cultivated Coffea species by generating the highest rate of both universality and discrimination power”
Claim · model-written summary
ITS efficiently discriminated Coffea liberica.
- Taxon
- Coffea liberica
Coffea libericae3 - Gene
- ITSe2
- Outcome
- efficiently discriminatede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Although ITS had lower resolution in Coffea species”
e3“it efficiently discriminated Coffea liberica”
Claim · model-written summary
The matK barcode discriminated all the Coffea species examined.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Molecular Authentication of Commercially Cultivated Coffee (Coffea spp.) in the Philippines using DNA Barcodes”
e4“The matK barcode discriminated all the species”
Claim · model-written summary
matK had the highest universality and discrimination power of the two barcodes, compared with ITS.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Molecular Authentication of Commercially Cultivated Coffee (Coffea spp.) in the Philippines using DNA Barcodes”
e5“matK is an efficient barcode over ITS for commercially cultivated Coffea species by generating the highest rate of both universality and discrimination power”
Claim · model-written summary
The authors state that DNA barcoding as an authentication method will benefit the coffee industry and growers.
- Outcome
- DNA barcoding as a method of authenticatione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Molecular Authentication of Commercially Cultivated Coffee (Coffea spp.) in the Philippines using DNA Barcodes”
e6“DNA barcoding as a method of authentication will benefit the coffee industry and coffee growers for large-scale plantations”