Paper · 2025 · Agronomy

Enhancing Maize–Climbing Bean Intercropping with Biostimulants: Implications for Yield and Silage Quality

Rafał Górski; Anna Sikorska; Robert Czaplicki; et al.

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citations
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Open access · cc-by

Abstract

In the face of climate pressure and threats to biodiversity, intercropping cereals with legumes and using biostimulants can increase feed yield and quality. This research evaluated a two-year intercropping system of maize and climbing beans for silage in central Poland, comparing four sowing schemes 90,000 ha−1 maize with 90,000 (90 + 90); 45,000 (90 + 45) or 27,500 (90 + 27.5) climbing beans ha−1 and sole maize, as well as five biostimulant application: control object, liquid microelement fertilizer (Zn-8.0%) containing zinc acetate, liquid extract from Ecklonia maxima algae, Methylobacterium symbioticum bacteria, Bacillus halotolerans bacteria. The aim of the field research was to evaluate the biomass components, yields, and crude protein content in silage. The intercropping pattern and biostimulants had a significant effect on dry matter and yields, with limited interactions. Single maize plant weight and yield were highest in the single crop and 90 + 27.5 treatments, while total intercrop yield peaked at 90 + 45, exceeding single maize by 14%. Biostimulants increased maize yields by 3–8% and intercrop yields by up to 6%, but reduced bean yields compared to controls. The crude protein content of silage was lowest for maize alone and highest for 90 + 45; biostimulants increased protein content by 5–9%, mainly for Methylobacterium symbioticum. Overall, the combination of 90 + 45 with Ecklonia maxima or Methylobacterium symbioticum optimized silage biomass and protein. The presented research is the first to evaluate the intercropping of maize with runner beans in orderly sowing and under the influence of biostimulants. It may constitute an important step in improving the efficiency of intercropping for implementation in agricultural practice. Further research should evaluate reduced mineral fertilization in this system.

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Claims 10

Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Intercropping pattern and biostimulants significantly affected dry matter and yields, with limited interactions.

Treatment
intercropping pattern and biostimulantse0
Outcome
dry matter and yieldse0
Qualifier
limited interactionse0

Not reportedTaxonDirection

Verbatim

e0

“The intercropping pattern and biostimulants had a significant effect on dry matter and yields, with limited interactions.”

✓ located verbatim in the source · abstract, characters 770–891 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Single maize plant weight and yield were highest under sole cropping and the 90 + 27.5 pattern.

Taxon
maizeZea mayse0
Treatment
single crop and 90 + 27.5 treatmentse0
Outcome
Single maize plant weight and yielde0
Direction
higheste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Single maize plant weight and yield were highest in the single crop and 90 + 27.5 treatments”

✓ located verbatim in the source · abstract, characters 892–984 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The 90 + 45 pattern produced the highest total intercrop yield, 14% above sole maize.

Taxon
Maize–Climbing Beant0
Treatment
90 + 45e0
Outcome
total intercrop yielde0
Direction
exceedinge0
Value
14%e0
Comparator
single maizee0

Verbatim

e0

“while total intercrop yield peaked at 90 + 45, exceeding single maize by 14%.”

✓ located verbatim in the source · abstract, characters 986–1063 · show in text ↑
t0

“Maize–Climbing Bean”

✓ located verbatim in the source · title, characters 10–29 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Biostimulants raised maize yields by 3–8%.

Taxon
maizeZea mayse0
Treatment
Biostimulantse0
Outcome
maize yieldse0
Direction
increasede0
Value
3–8%e0
Comparator
controlse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Biostimulants increased maize yields by 3–8% and intercrop yields by up to 6%, but reduced bean yields compared to controls.”

✓ located verbatim in the source · abstract, characters 1064–1188 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Biostimulants raised intercrop yields by up to 6%.

Taxon
Maize–Climbing Beant0
Treatment
Biostimulantse0
Outcome
intercrop yieldse0
Direction
increasede0
Value
up to 6%e0
Comparator
controlse0

Verbatim

e0

“Biostimulants increased maize yields by 3–8% and intercrop yields by up to 6%, but reduced bean yields compared to controls.”

✓ located verbatim in the source · abstract, characters 1064–1188 · show in text ↑
t0

“Maize–Climbing Bean”

✓ located verbatim in the source · title, characters 10–29 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Biostimulants reduced bean yields relative to controls.

Taxon
beane0
Treatment
Biostimulantse0
Outcome
bean yieldse0
Direction
reducede0
Comparator
controlse0

Verbatim

e0

“Biostimulants increased maize yields by 3–8% and intercrop yields by up to 6%, but reduced bean yields compared to controls.”

✓ located verbatim in the source · abstract, characters 1064–1188 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Silage crude protein was lowest with sole maize and highest with the 90 + 45 pattern.

Taxon
maizeZea mayse0
Treatment
90 + 45e0
Outcome
crude protein content of silagee0
Direction
higheste0
Comparator
maize alonee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The crude protein content of silage was lowest for maize alone and highest for 90 + 45”

✓ located verbatim in the source · abstract, characters 1189–1275 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Biostimulants increased silage protein by 5–9%, chiefly with Methylobacterium symbioticum.

Taxon
Maize–Climbing Beant0
Treatment
biostimulantse0
Interactor
Methylobacterium symbioticumMethylobacterium symbioticume0symbiont
Outcome
protein contente0
Direction
increasede0
Value
5–9%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Methylobacterium symbioticum
Methylobacterium symbioticum · InteractorSymborg · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“biostimulants increased protein content by 5–9%, mainly for Methylobacterium symbioticum.”

✓ located verbatim in the source · abstract, characters 1277–1366 · show in text ↑
t0

“Maize–Climbing Bean”

✓ located verbatim in the source · title, characters 10–29 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors judged 90 + 45 with Ecklonia maxima to optimize silage biomass and protein.

Taxon
Maize–Climbing Beant0
Treatment
90 + 45 with Ecklonia maximae0
Outcome
silage biomass and proteine0

Verbatim

e0

“Overall, the combination of 90 + 45 with Ecklonia maxima or Methylobacterium symbioticum optimized silage biomass and protein.”

✓ located verbatim in the source · abstract, characters 1367–1493 · show in text ↑
t0

“Maize–Climbing Bean”

✓ located verbatim in the source · title, characters 10–29 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors judged 90 + 45 with Methylobacterium symbioticum to optimize silage biomass and protein.

Taxon
Maize–Climbing Beant0
Treatment
Methylobacterium symbioticume0
Interactor
Methylobacterium symbioticumMethylobacterium symbioticume0symbiont
Outcome
silage biomass and proteine0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Methylobacterium symbioticum
Methylobacterium symbioticum · InteractorSymborg · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Overall, the combination of 90 + 45 with Ecklonia maxima or Methylobacterium symbioticum optimized silage biomass and protein.”

✓ located verbatim in the source · abstract, characters 1367–1493 · show in text ↑
t0

“Maize–Climbing Bean”

✓ located verbatim in the source · title, characters 10–29 · show in text ↑

other · yield · Phaseolus vulgaris (common bean)Results differ

44 papers: 40 positive, 4 negative or no effect

3 papers in the same direction as this paper · 40 in the opposite direction

  1. positive
  2. positive
  3. positive

40 papers more in this group

See all 44 papers →

other · yield · Zea mays (maize)Positive and negative results

253 papers: 221 positive, 32 negative or no effect

220 papers in the same direction as this paper · 26 in the opposite direction · 6 reporting no difference from an alternative

  1. no effect
  2. no effect
  3. no effect

249 papers more in this group

See all 253 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.