Abstract
Light stimulates anthocyanin accumulation in bicolored sweet cherry (Prunus avium cv. Rainier) fruits, resulting in red pigmentation. The phytohormone gibberellin (GA) plays an important role in fruit coloration, but the molecular mechanisms that integrate light and GA signaling pathways during anthocyanin biosynthesis remain poorly understood. We show that light induces anthocyanin accumulation but reduces the gibberellic acid (GA3) content in "Rainier" sweet cherry fruits and that GA3 treatment inhibits light-induced anthocyanin accumulation. Light exposure and low GA3 levels induce the transcription of a DEHYDRATION RESPONSIVE ELEMENT-BINDING PROTEIN (DREB) gene, PavDREB1E, which encodes a protein that directly activates the transcription of key anthocyanin biosynthesis genes, including chalcone synthase (PavCHS), dihydroflavonol-4-reductase (PavDFR), and UDP glucose-flavonoid-3-O-glycosyltransferase (PavUFGT). The DELLA protein PavDWARF8 (PavD8), which is stabilized under low GA3 levels, interacts with PavDREB1E to strengthen its transcriptional activation activity. Furthermore, light-induced ELONGATED HYPOCOTYL5 (PavHY5) promotes this interaction. In the dark, PavDREB1E and PavHY5 transcript levels are low. Moreover, PavDREB1E, PavD8, and PavHY5 are degraded in the dark and accumulate in the light via the light-induced nuclear depletion of the E3 ubiquitin ligases CONSTITUTIVELY PHOTOMORPHOGENIC 1 (PavCOP1-1 and PavCOP1-2). We present a mechanism by which the PavDREB1E-PavD8-PavHY5 module integrates light and GA signaling to regulate anthocyanin biosynthesis in sweet cherry fruits.
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Claims 12
Claim · model-written summary
Light induced anthocyanin accumulation in Rainier sweet cherry fruit.
- Taxon
- sweet cherry
Prunus aviume0 - Material
- "Rainier"e0
- Organ / stage
- fruitse0
- Treatment
- lighte0
- Outcome
- anthocyanin accumulatione0
- Direction
- inducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“light induces anthocyanin accumulation but reduces the gibberellic acid (GA3) content in "Rainier" sweet cherry fruits”
Claim · model-written summary
Light reduced GA3 content in Rainier sweet cherry fruit.
- Taxon
- sweet cherryPrunus aviume0
- Material
- "Rainier"e0
- Organ / stage
- fruitse0
- Treatment
- lighte0
- Outcome
- gibberellic acid (GA3) contente0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“light induces anthocyanin accumulation but reduces the gibberellic acid (GA3) content in "Rainier" sweet cherry fruits”
Claim · model-written summary
GA3 treatment inhibited light-induced anthocyanin accumulation.
- Taxon
- sweet cherryPrunus aviumt0
- Treatment
- GA3 treatmente0
- Outcome
- light-induced anthocyanin accumulatione0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GA3 treatment inhibits light-induced anthocyanin accumulation”
t0“sweet cherry”
Claim · model-written summary
Light exposure induced PavDREB1E transcription.
- Taxon
- sweet cherryPrunus aviumt0
- Treatment
- Light exposuree0
- Gene
- PavDREB1Ee0
- Outcome
- transcriptione0
- Direction
- inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Light exposure and low GA3 levels induce the transcription of a DEHYDRATION RESPONSIVE ELEMENT-BINDING PROTEIN (DREB) gene, PavDREB1E”
t0“sweet cherry”
Claim · model-written summary
Low GA3 levels induced PavDREB1E transcription.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Light exposure and low GA3 levels induce the transcription of a DEHYDRATION RESPONSIVE ELEMENT-BINDING PROTEIN (DREB) gene, PavDREB1E”
t0“sweet cherry”
Claim · model-written summary
PavDREB1E directly activated transcription of PavCHS, PavDFR and PavUFGT.
- Taxon
- sweet cherryPrunus aviumt0
- Gene
- PavDREB1Ee0
- Outcome
- transcription of key anthocyanin biosynthesis genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PavDREB1E, which encodes a protein that directly activates the transcription of key anthocyanin biosynthesis genes, including chalcone synthase (PavCHS), dihydroflavonol-4-reductase (PavDFR), and UDP glucose-flavonoid-3-O-glycosyltransferase (PavUFGT)”
t0“sweet cherry”
Claim · model-written summary
Low GA3 levels stabilized PavD8.
- Taxon
- sweet cherryPrunus aviumt0
- Gene
- PavDWARF8 (PavD8)e0
- Compound
- GA3e0
- Outcome
- stabilizede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The DELLA protein PavDWARF8 (PavD8), which is stabilized under low GA3 levels”
t0“sweet cherry”
Claim · model-written summary
PavD8 interacted with PavDREB1E and strengthened its transcriptional activation.
- Taxon
- sweet cherryPrunus aviumt0
- Gene
- PavDREB1Ee0
- Outcome
- transcriptional activation activitye0
- Qualifier
- interacts with PavDREB1Ee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“interacts with PavDREB1E to strengthen its transcriptional activation activity”
t0“sweet cherry”
Claim · model-written summary
Light-induced PavHY5 promoted the PavD8–PavDREB1E interaction.
- Taxon
- sweet cherryPrunus aviumt0
- Gene
- ELONGATED HYPOCOTYL5 (PavHY5)e0
- Outcome
- promotes this interactione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“light-induced ELONGATED HYPOCOTYL5 (PavHY5) promotes this interaction”
t0“sweet cherry”
Claim · model-written summary
PavDREB1E and PavHY5 transcript levels were low in darkness.
- Taxon
- sweet cherryPrunus aviumt0
- Gene
- PavDREB1E and PavHY5e0
- Outcome
- transcript levels are lowe0
- Setting
- In the darke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the dark, PavDREB1E and PavHY5 transcript levels are low”
t0“sweet cherry”
Claim · model-written summary
PavDREB1E, PavD8 and PavHY5 were degraded in darkness.
- Taxon
- sweet cherryPrunus aviumt0
- Gene
- PavDREB1E, PavD8, and PavHY5e0
- Outcome
- degradede0
- Setting
- in the darke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PavDREB1E, PavD8, and PavHY5 are degraded in the dark”
t0“sweet cherry”
Claim · model-written summary
PavDREB1E, PavD8 and PavHY5 accumulated in light through nuclear depletion of PavCOP1 ligases.
- Taxon
- sweet cherryPrunus aviumt0
- Gene
- PavCOP1-1 and PavCOP1-2e0
- Outcome
- accumulatee0
- Qualifier
- light-induced nuclear depletione0
- Setting
- in the lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“accumulate in the light via the light-induced nuclear depletion of the E3 ubiquitin ligases CONSTITUTIVELY PHOTOMORPHOGENIC 1 (PavCOP1-1 and PavCOP1-2)”
t0“sweet cherry”
The same questions in other papers
Gibberellic acid · quality: phytochemicals · Prunus avium (sweet cherry)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
light · quality: phytochemicals · Prunus avium (sweet cherry)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.