Paper · 2025 · Technology in Horticulture

Four herbal microgreen species varied in optimal seeding densities and growing substrate for maximized shoot yield and mineral nutrients

Jacob Arthur; Tongyin Li; Guihong Bi; et al.

8
claims
8
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by

Abstract

The microgreen industry in North America has experienced continuous growth, with an increasing number of species, including vegetables, herbs, and grains, being produced as microgreens. Cultural practices significantly impact the productivity and nutrient composition of various microgreen species. The hypothesis of this study was that optimal cultural practices, including seeding density and substrate type in microgreen production, should be species dependent. This study investigated shoot growth, visual quality, and mineral nutrient compositions of four herbal microgreen species, including basil, chives, scallion, and shiso, in two experiments as affected by five seeding densities, including 75, 150, 225, 300, and 375 g·m−2, when grown with a peat-based soilless substrate, and a hydroponic mat made from jute fibers. Microgreen fresh shoot weights generally increased with increasing seeding density, with scallion producing the highest fresh shoot weight at 375 g·m−2, whereas the 375 g·m−2 seeding density increased fresh shoot weight compared with 75 to 225 g·m−2, but resulted in similar fresh shoot yield to 300 g·m−2 in basil, chives, and shiso. Scallion and shiso microgreens produced higher fresh shoot weight when grown on peat-based substrate, while basil and chives produced higher fresh shoot weight on jute mat compared with peat in one or both experiments. Substrate type also altered mineral nutrient concentrations in tested microgreens with peat substrate increasing phosphorus (P), potassium (K), calcium (Ca), and sulfur (S), and jute mat increasing nitrogen (N) and magnesium (Mg) in one or more species.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 8

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Scallion produced the highest fresh shoot weight at 375 g·m−2 seeding density.

Taxon
scallione1
Treatment
seeding densitye1
Outcome
fresh shoot weighte1
Direction
higheste1
Value
375 g·m−2e1

Not reportedComparator

Verbatim

e1

“Microgreen fresh shoot weights generally increased with increasing seeding density, with scallion producing the highest fresh shoot weight at 375 g·m−2”

✓ located verbatim in the source · abstract, characters 829–980 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The 375 g·m−2 seeding density increased fresh shoot weight of basil, chives and shiso compared with 75 to 225 g·m−2.

Treatment
375 g·m−2 seeding densitye1
Outcome
fresh shoot weighte1
Direction
increasede1
Comparator
75 to 225 g·m−2e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗
Allium schoenoprasum
Allium schoenoprasum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Perilla frutescens var. crispa
Perilla frutescens var. crispa · TaxonDalgial · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“the 375 g·m−2 seeding density increased fresh shoot weight compared with 75 to 225 g·m−2, but resulted in similar fresh shoot yield to 300 g·m−2 in basil, chives, and shiso”

✓ located verbatim in the source · abstract, characters 990–1162 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

The 375 g·m−2 seeding density gave fresh shoot yield similar to 300 g·m−2 in basil, chives and shiso.

Treatment
375 g·m−2 seeding densitye1
Outcome
fresh shoot yielde1
Direction
similare1
Comparator
300 g·m−2e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗
Allium schoenoprasum
Allium schoenoprasum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Perilla frutescens var. crispa
Perilla frutescens var. crispa · TaxonDalgial · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“the 375 g·m−2 seeding density increased fresh shoot weight compared with 75 to 225 g·m−2, but resulted in similar fresh shoot yield to 300 g·m−2 in basil, chives, and shiso”

✓ located verbatim in the source · abstract, characters 990–1162 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Scallion and shiso produced higher fresh shoot weight on peat-based substrate.

Taxon
Scallion and shisoe1
Treatment
peat-based substratee1
Outcome
fresh shoot weighte1
Direction
highere1

Not reportedComparator

Verbatim

e1

“Scallion and shiso microgreens produced higher fresh shoot weight when grown on peat-based substrate, while basil and chives produced higher fresh shoot weight on jute mat compared with peat in one or both experiments”

✓ located verbatim in the source · abstract, characters 1164–1381 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Basil and chives produced higher fresh shoot weight on jute mat than on peat in one or both experiments.

Treatment
jute mate1
Outcome
fresh shoot weighte1
Direction
highere1
Qualifier
in one or both experimentse1
Comparator
peate1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗
Allium schoenoprasum
Allium schoenoprasum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Scallion and shiso microgreens produced higher fresh shoot weight when grown on peat-based substrate, while basil and chives produced higher fresh shoot weight on jute mat compared with peat in one or both experiments”

✓ located verbatim in the source · abstract, characters 1164–1381 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Peat substrate increased phosphorus, potassium and calcium concentrations in tested microgreens.

Taxon
tested microgreense1
Treatment
peat substratee1
Outcome
phosphorus (P), potassium (K), calcium (Ca)e1
Direction
increasinge1

Not reportedComparator

Verbatim

e1

“Substrate type also altered mineral nutrient concentrations in tested microgreens with peat substrate increasing phosphorus (P), potassium (K), calcium (Ca), and sulfur (S), and jute mat increasing nitrogen (N) and magnesium (Mg) in one or more species”

✓ located verbatim in the source · abstract, characters 1383–1635 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Peat substrate increased sulfur concentration in tested microgreens.

Taxon
tested microgreense1
Treatment
peat substratee1
Outcome
sulfur (S)e1
Direction
increasinge1

Not reportedComparator

Verbatim

e1

“Substrate type also altered mineral nutrient concentrations in tested microgreens with peat substrate increasing phosphorus (P), potassium (K), calcium (Ca), and sulfur (S), and jute mat increasing nitrogen (N) and magnesium (Mg) in one or more species”

✓ located verbatim in the source · abstract, characters 1383–1635 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Jute mat increased nitrogen and magnesium concentrations in one or more microgreen species.

Taxon
tested microgreense1
Treatment
jute mate1
Outcome
nitrogen (N) and magnesium (Mg)e1
Direction
increasinge1
Qualifier
in one or more speciese1

Not reportedComparator

Verbatim

e1

“Substrate type also altered mineral nutrient concentrations in tested microgreens with peat substrate increasing phosphorus (P), potassium (K), calcium (Ca), and sulfur (S), and jute mat increasing nitrogen (N) and magnesium (Mg) in one or more species”

✓ located verbatim in the source · abstract, characters 1383–1635 · show in text ↑

density · yield · Allium schoenoprasum (chive)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

density · yield · Ocimum basilicum (basil)Positive and negative results

10 papers: 7 positive, 3 negative or no effect

2 papers in the same direction as this paper · 7 in the opposite direction

  1. positive
  2. positive
  3. positive

6 papers more in this group

See all 10 papers →

density · growth · Perilla frutescens (beefsteak plant)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

density · growth · Ocimum basilicum (basil)Results agree

8 papers: 8 positive, 0 negative or no effect

7 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

4 papers more in this group

See all 8 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.