Abstract
Abstract Grafting in watermelon using traditional methods often causes rootstock regrowth, increasing labor demand and production costs. Although cotyledon-less splice grafting eliminates regrowth by excising meristem tissue, its success rate has consistently been lower. Here, we developed a novel cotyledon-less splice grafting methodology that achieved high survival rates by modulating pre-grafting light intensities from 100 to 300 μmol·m−2·s−1 for scion and rootstock, generating four experimental groups: high-light intensity scion/high-light intensity rootstock (HS/HR), high-light intensity scion/low-light intensity rootstock (HS/LR), low-light intensity scion/high-light intensity rootstock (LS/HR), and low-light intensity scion/low-light intensity rootstock (LS/LR). The results demonstrated that HS/HR and LS/HR exhibited the highest survival rates, nearly 98%, and displayed high seedling quality, markedly enhanced graft-union adhesion, and accelerated vascular reconnection. Pretreatment of high light intensity increased starch accumulation in rootstock hypocotyls, enhancing tolerance to carbon starvation after grafting especially in the cotyledon-less grafts. Metabolomic analysis identified elevated levels of key metabolites, including auxins, cytokinins, D-galactose, galactinol, starch, cinnamic acid, M-coumaric acid, and vanilloloside. Transcriptomic profiling revealed significant enrichment of plant hormone signal, starch and sucrose metabolism, and phenylpropanoid biosynthesis pathways in scion and rootstock tissues underpinning hormonal regulation, carbohydrate metabolism, and lignin biosynthesis under high-light conditions. WGCNA identified key co-expression modules associated with graft healing traits and key metabolites. Furthermore, graft healing related genes (PXY, NAC086, CALS7, and TMO6) were upregulated. In conclusion, our findings underscore the critical role of light intensity in orchestrating transcriptional and metabolic networks to optimize graft healing, providing a physiological and molecular foundation for improving cotyledon-less grafting efficiency.
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Claims 11
Claim · model-written summary
HS/HR and LS/HR had the highest survival, nearly 98%.
- Taxon
- watermelon
Citrullus lanatust0 - Treatment
- HS/HR and LS/HRe0
- Outcome
- survival ratese0
- Direction
- higheste0
- Value
- nearly 98%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HS/HR and LS/HR exhibited the highest survival rates, nearly 98%”
t0“watermelon”
Claim · model-written summary
HS/HR and LS/HR produced high seedling quality and improved graft adhesion and vascular reconnection.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- HS/HR and LS/HRe0
- Outcome
- seedling quality, markedly enhanced graft-union adhesion, and accelerated vascular reconnectione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HS/HR and LS/HR exhibited the highest survival rates, nearly 98%, and displayed high seedling quality, markedly enhanced graft-union adhesion, and accelerated vascular reconnection.”
t0“watermelon”
Claim · model-written summary
High-light pretreatment increased starch accumulation in rootstock hypocotyls.
- Taxon
- watermelonCitrullus lanatust0
- Organ / stage
- rootstock hypocotylse0
- Treatment
- high light intensitye0
- Outcome
- starch accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pretreatment of high light intensity increased starch accumulation in rootstock hypocotyls”
t0“watermelon”
Claim · model-written summary
Increased starch accumulation enhanced tolerance to carbon starvation after grafting.
- Taxon
- watermelonCitrullus lanatust0
- Outcome
- tolerance to carbon starvatione0
- Qualifier
- starch accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased starch accumulation in rootstock hypocotyls, enhancing tolerance to carbon starvation after grafting especially in the cotyledon-less grafts.”
t0“watermelon”
Claim · model-written summary
Metabolomics found elevated auxins, cytokinins and D-galactose.
- Taxon
- watermelonCitrullus lanatust0
- Outcome
- elevated levels of key metabolites, including auxins, cytokinins, D-galactosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Metabolomic analysis identified elevated levels of key metabolites, including auxins, cytokinins, D-galactose, galactinol, starch, cinnamic acid, M-coumaric acid, and vanilloloside.”
t0“watermelon”
Claim · model-written summary
Metabolomics found elevated galactinol, starch and cinnamic acid.
- Taxon
- watermelonCitrullus lanatust0
- Outcome
- galactinol, starch, cinnamic acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Metabolomic analysis identified elevated levels of key metabolites, including auxins, cytokinins, D-galactose, galactinol, starch, cinnamic acid, M-coumaric acid, and vanilloloside.”
t0“watermelon”
Claim · model-written summary
Metabolomics found elevated M-coumaric acid and vanilloloside.
- Taxon
- watermelonCitrullus lanatust0
- Outcome
- M-coumaric acid, and vanillolosidee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Metabolomic analysis identified elevated levels of key metabolites, including auxins, cytokinins, D-galactose, galactinol, starch, cinnamic acid, M-coumaric acid, and vanilloloside.”
t0“watermelon”
Claim · model-written summary
Transcriptomics found significant enrichment of hormone signaling, starch and sucrose metabolism, and phenylpropanoid biosynthesis pathways.
- Taxon
- watermelonCitrullus lanatust0
- Organ / stage
- scion and rootstock tissuese0
- Outcome
- significant enrichment of plant hormone signal, starch and sucrose metabolism, and phenylpropanoid biosynthesis pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptomic profiling revealed significant enrichment of plant hormone signal, starch and sucrose metabolism, and phenylpropanoid biosynthesis pathways in scion and rootstock tissues”
t0“watermelon”
Claim · model-written summary
WGCNA identified co-expression modules associated with graft healing traits and metabolites.
- Taxon
- watermelonCitrullus lanatust0
- Gene
- co-expression modulese0
- Outcome
- graft healing traitse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WGCNA identified key co-expression modules associated with graft healing traits and key metabolites.”
t0“watermelon”
Claim · model-written summary
PXY, NAC086 and CALS7 were upregulated.
- Taxon
- watermelonCitrullus lanatust0
- Gene
- PXY, NAC086, CALS7e0
- Outcome
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“graft healing related genes (PXY, NAC086, CALS7, and TMO6) were upregulated.”
t0“watermelon”
Claim · model-written summary
TMO6 was upregulated.
- Taxon
- watermelonCitrullus lanatust0
- Gene
- TMO6e0
- Outcome
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“graft healing related genes (PXY, NAC086, CALS7, and TMO6) were upregulated.”
t0“watermelon”
The same questions in other papers
light · quality: sugar starch · Citrullus lanatus (watermelon)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.