Abstract
The coupling of partial root-zone drying (PRD) with nitrogen forms exerts an interactive “water-promoted fertilization” effect, which enhances rice (Oryza sativa L.) growth and development, improves water use efficiency (WUE), mediates the expression of aquaporins (AQPs), and alters root water conductivity. In this study, gene cloning and CRISPR-Cas9 technologies were employed to construct overexpression and knockout vectors of the OsPIP2;1 gene, which were then transformed into rice (cv. Meixiangzhan 2). Three water treatments were set: normal irrigation (CK); partial root-zone drying (PRD); and 10% PEG-simulated water stress (PEG), combined with a nitrogen form ratio of ammonium nitrogen (NH4+) to nitrate nitrogen (NO3−) at 50:50 (A50/N50) for the coupled treatment of rice seedlings. The results showed that under the coupled treatment of PRD and the aforementioned nitrogen form, the expression level of the OsPIP2;1 gene in roots was upregulated by 0.62-fold on the seventh day, while its expression level in leaves was downregulated by 1.84-fold. Overexpression of OsPIP2;1 enabled Meixiangzhan 2 to maintain a higher abscisic acid (ABA) level under different water conditions, which helped rice reduce water potential and enhance water absorption. Compared with the CK treatment, overexpression of OsPIP2;1 increased the superoxide dismutase (SOD) activity of rice under PRD by 26.98%, effectively alleviating tissue damage caused by excessive accumulation of O2−. The physiological and biochemical characteristics of OsPIP2;1-overexpressing rice showed correlations under PRD and A50/N50 nitrogen form conditions, with WUE exhibiting a significant positive correlation with transpiration rate, chlorophyll content, nitrogen content, and Rubisco enzyme activity. Overexpression of OsPIP2;1 could promote root growth and increase the total biomass of rice plants. The application of the OsPIP2;1 gene in rice genetic engineering modification holds great potential for improving important agricultural traits of crops. This study provides new insights into the mechanism by which the AQP family regulates water use in rice and has certain significance for exploring the role of AQP genes in rice growth and development as well as in response to water stress.
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Claims 8
Claim · model-written summary
Coupled PRD and nitrogen treatment upregulated root OsPIP2;1 expression by 0.62-fold on day seven.
- Taxon
- rice
Oryza sativae1 - Organ / stage
- rootse0
- Treatment
- coupled treatment of PRD and the aforementioned nitrogen forme0
- Gene
- OsPIP2;1e0
- Outcome
- expression level of the OsPIP2;1 gene in rootse0
- Direction
- upregulatede0
- Value
- 0.62-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“under the coupled treatment of PRD and the aforementioned nitrogen form, the expression level of the OsPIP2;1 gene in roots was upregulated by 0.62-fold on the seventh day”
e1“rice (Oryza sativa L.) growth and development”
Claim · model-written summary
Coupled PRD and nitrogen treatment downregulated leaf OsPIP2;1 expression by 1.84-fold.
- Taxon
- riceOryza sativae2
- Organ / stage
- leavese0
- Treatment
- coupled treatment of PRD and the aforementioned nitrogen forme1
- Gene
- OsPIP2;1e1
- Outcome
- expression level in leavese0
- Direction
- downregulatede0
- Value
- 1.84-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its expression level in leaves was downregulated by 1.84-fold”
e1“coupled treatment of PRD and the aforementioned nitrogen form, the expression level of the OsPIP2;1 gene in roots”
e2“rice (Oryza sativa L.)”
Claim · model-written summary
OsPIP2;1 overexpression maintained higher ABA levels under different water conditions.
- Taxon
- riceOryza sativae1
- Material
- Meixiangzhan 2e0
- Treatment
- Overexpression of OsPIP2;1e0
- Outcome
- abscisic acid (ABA) levele0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of OsPIP2;1 enabled Meixiangzhan 2 to maintain a higher abscisic acid (ABA) level under different water conditions”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
OsPIP2;1 overexpression reduced water potential and enhanced water absorption.
- Taxon
- riceOryza sativae0
- Treatment
- Overexpression of OsPIP2;1e1
- Outcome
- water potential and enhance water absorptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“helped rice reduce water potential and enhance water absorption”
e1“Overexpression of OsPIP2;1 enabled Meixiangzhan 2”
Claim · model-written summary
OsPIP2;1 overexpression raised SOD activity under PRD by 26.98% versus CK.
- Taxon
- riceOryza sativae0
- Treatment
- overexpression of OsPIP2;1e0
- Outcome
- superoxide dismutase (SOD) activitye0
- Direction
- increasede0
- Value
- 26.98%e0
- Comparator
- CK treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with the CK treatment, overexpression of OsPIP2;1 increased the superoxide dismutase (SOD) activity of rice under PRD by 26.98%”
Claim · model-written summary
WUE correlated positively with transpiration rate, chlorophyll and nitrogen content, and Rubisco activity under PRD and A50/N50.
- Taxon
- riceOryza sativae1
- Treatment
- PRD and A50/N50 nitrogen form conditionse0
- Outcome
- WUEe0
- Direction
- positive correlatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“under PRD and A50/N50 nitrogen form conditions, with WUE exhibiting a significant positive correlation with transpiration rate, chlorophyll content, nitrogen content, and Rubisco enzyme activity”
e1“OsPIP2;1-overexpressing rice”
Claim · model-written summary
OsPIP2;1 overexpression promoted root growth.
- Taxon
- riceOryza sativae0
- Treatment
- Overexpression of OsPIP2;1e0
- Outcome
- root growthe0
- Direction
- promotee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of OsPIP2;1 could promote root growth and increase the total biomass of rice plants”
Claim · model-written summary
OsPIP2;1 overexpression increased total rice biomass.
- Taxon
- riceOryza sativae0
- Treatment
- Overexpression of OsPIP2;1e0
- Outcome
- total biomasse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of OsPIP2;1 could promote root growth and increase the total biomass of rice plants”
The same questions in other papers
nitrogen · molecular: expression · Oryza sativa (rice)Positive and negative results
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- no effect
- negative
- negative
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See all 45 papers →irrigation · molecular: expression · Oryza sativa (rice)Results agree
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- positive
- positive
- positive
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.