Paper · 2025 · Soil & Environment

Impact of microbial consortium along with biochar and pressmud on mashbean yield and seed quality in field conditions

Kashiuf Asif; Hafiz Naeem Asghar; Ejaz Ahmad Waraich

8
claims
19
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by

Abstract

Application of organic matter sources along with microbial formulations stimulates microbial activities and their potential to support plant growth, and enhances plant survival under field conditions. We evaluated the impact of different rhizobial and rhizobacterial strains in sustaining plant growth under field conditions by using Vigna mungo as test crop. Unlike most previous research that examine single microbial inoculants or overlook the role of local organic amendments, current study evaluated the consortium of indigenous rhizobium and PGPR with biochar and pressmud to synergistically improve mash bean growth, yield and quality. 16S rRNA sequencing revealed Rhizobium sp., ‘Stenotrophomonas rhizophila’ yielding most efficient outcomes followed by Bacillus subtilis, and Bradyrhizobium japonicum. Co-inoculation of bacteria (S. rhizophila and Rhizobium sp.) along with 0.5% orange peel biochar and 5 tons/ha pressmud proved to be the most efficient in this regard. Treatment (T5) exhibited the greatest increase in plant height (51%), plant fresh weight (127%), plant dry weight (174%), 1000-grains weight (37.4%) than control. Next best increase Bacillus subtilis, Bradyrhizobium japonicum, 0.25% biochar, 10 tons/ha pressmud yielded the next best increase in grain yield (37.4%). Maximum crude protein (54%) was observed with inoculation of strain ‘PKA-18, RKA-47, 0.5% biochar, 5 Tons ha-1 pressmud as compared to un-inoculated control. Among all the tested isolates, co-inoculation of T5 followed by T4 was the most efficient treatment in improving mash bean growth, yield, and seed quality.

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Claims 8

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Treatment T5 gave the greatest increase in plant height of Vigna mungo (51%) relative to control.

Taxon
Vigna mungoVigna mungoe0
Treatment
T5e1
Interactor
Rhizobium sp.Rhizobiume2symbiont
Outcome
plant heighte1
Direction
greatest increasee1
Value
51%e1
Comparator
controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna mungo
Vigna mungo · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗
Rhizobium
Rhizobium · Interactor · picture of the genus (one of its species)Ninjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“by using Vigna mungo as test crop”

✓ located verbatim in the source · abstract, characters 325–358 · show in text ↑
e1

“Treatment (T5) exhibited the greatest increase in plant height (51%), plant fresh weight (127%), plant dry weight (174%), 1000-grains weight (37.4%) than control”

✓ located verbatim in the source · abstract, characters 979–1140 · show in text ↑
e2

“Co-inoculation of bacteria (S. rhizophila and Rhizobium sp.) along with 0.5% orange peel biochar and 5 tons/ha pressmud proved to be the most efficient in this regard”

✓ located verbatim in the source · abstract, characters 811–977 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Treatment T5 gave the greatest increase in plant fresh weight of Vigna mungo (127%) relative to control.

Taxon
Vigna mungoVigna mungoe0
Treatment
T5e1
Interactor
Rhizobium sp.Rhizobiume2symbiont
Outcome
plant fresh weighte1
Direction
greatest increasee1
Value
127%e1
Comparator
controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna mungo
Vigna mungo · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗
Rhizobium
Rhizobium · Interactor · picture of the genus (one of its species)Ninjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“by using Vigna mungo as test crop”

✓ located verbatim in the source · abstract, characters 325–358 · show in text ↑
e1

“Treatment (T5) exhibited the greatest increase in plant height (51%), plant fresh weight (127%), plant dry weight (174%), 1000-grains weight (37.4%) than control”

✓ located verbatim in the source · abstract, characters 979–1140 · show in text ↑
e2

“Co-inoculation of bacteria (S. rhizophila and Rhizobium sp.) along with 0.5% orange peel biochar and 5 tons/ha pressmud proved to be the most efficient in this regard”

✓ located verbatim in the source · abstract, characters 811–977 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Treatment T5 gave the greatest increase in plant dry weight of Vigna mungo (174%) relative to control.

Taxon
Vigna mungoVigna mungoe0
Treatment
T5e1
Interactor
Rhizobium sp.Rhizobiume2symbiont
Outcome
plant dry weighte1
Direction
greatest increasee1
Value
174%e1
Comparator
controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna mungo
Vigna mungo · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗
Rhizobium
Rhizobium · Interactor · picture of the genus (one of its species)Ninjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“by using Vigna mungo as test crop”

✓ located verbatim in the source · abstract, characters 325–358 · show in text ↑
e1

“Treatment (T5) exhibited the greatest increase in plant height (51%), plant fresh weight (127%), plant dry weight (174%), 1000-grains weight (37.4%) than control”

✓ located verbatim in the source · abstract, characters 979–1140 · show in text ↑
e2

“Co-inoculation of bacteria (S. rhizophila and Rhizobium sp.) along with 0.5% orange peel biochar and 5 tons/ha pressmud proved to be the most efficient in this regard”

✓ located verbatim in the source · abstract, characters 811–977 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Treatment T5 gave the greatest increase in 1000-grain weight of Vigna mungo (37.4%) relative to control.

Taxon
Vigna mungoVigna mungoe0
Treatment
T5e1
Interactor
Rhizobium sp.Rhizobiume2symbiont
Outcome
1000-grains weighte1
Direction
greatest increasee1
Value
37.4%e1
Comparator
controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna mungo
Vigna mungo · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗
Rhizobium
Rhizobium · Interactor · picture of the genus (one of its species)Ninjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“by using Vigna mungo as test crop”

✓ located verbatim in the source · abstract, characters 325–358 · show in text ↑
e1

“Treatment (T5) exhibited the greatest increase in plant height (51%), plant fresh weight (127%), plant dry weight (174%), 1000-grains weight (37.4%) than control”

✓ located verbatim in the source · abstract, characters 979–1140 · show in text ↑
e2

“Co-inoculation of bacteria (S. rhizophila and Rhizobium sp.) along with 0.5% orange peel biochar and 5 tons/ha pressmud proved to be the most efficient in this regard”

✓ located verbatim in the source · abstract, characters 811–977 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The next best grain yield increase (37.4%) of Vigna mungo came from Bacillus subtilis, Bradyrhizobium japonicum, 0.25% biochar and 10 tons/ha pressmud.

Taxon
Vigna mungoVigna mungoe0
Treatment
Bacillus subtilis, Bradyrhizobium japonicum, 0.25% biochar, 10 tons/ha pressmude3
Interactor
Bacillus subtilisBacillus subtilise3symbiont
Outcome
grain yielde3
Direction
next best increasee3
Value
37.4%e3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna mungo
Vigna mungo · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗
Bacillus subtilis
Bacillus subtilis · InteractorY tambe (original uploader) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“by using Vigna mungo as test crop”

✓ located verbatim in the source · abstract, characters 325–358 · show in text ↑
e3

“Next best increase Bacillus subtilis, Bradyrhizobium japonicum, 0.25% biochar, 10 tons/ha pressmud yielded the next best increase in grain yield (37.4%)”

✓ located verbatim in the source · abstract, characters 1142–1294 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Strain PKA-18 and RKA-47 with 0.5% biochar and 5 t/ha pressmud gave the maximum crude protein (54%) of Vigna mungo compared with un-inoculated control.

Taxon
Vigna mungoVigna mungoe0
Treatment
PKA-18, RKA-47, 0.5% biochar, 5 Tons ha-1 pressmude4
Outcome
crude proteine4
Direction
Maximume4
Value
54%e4
Comparator
un-inoculated controle4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna mungo
Vigna mungo · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“by using Vigna mungo as test crop”

✓ located verbatim in the source · abstract, characters 325–358 · show in text ↑
e4

“Maximum crude protein (54%) was observed with inoculation of strain ‘PKA-18, RKA-47, 0.5% biochar, 5 Tons ha-1 pressmud as compared to un-inoculated control”

✓ located verbatim in the source · abstract, characters 1296–1452 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Co-inoculation of T5 was the most efficient treatment, ahead of T4, for mash bean growth, yield and seed quality among the tested isolates.

Taxon
mash beane5
Treatment
co-inoculation of T5e5
Outcome
growth, yield, and seed qualitye5
Direction
most efficiente5
Comparator
T4e5

Verbatim

e5

“Among all the tested isolates, co-inoculation of T5 followed by T4 was the most efficient treatment in improving mash bean growth, yield, and seed quality”

✓ located verbatim in the source · abstract, characters 1454–1608 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Stenotrophomonas rhizophila gave the most efficient outcomes among the tested strains, followed by Bacillus subtilis and Bradyrhizobium japonicum.

Taxon
Vigna mungoVigna mungoe0
Interactor
Stenotrophomonas rhizophilaStenotrophomonas rhizophilae6symbiont
Outcome
efficient outcomese6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna mungo
Vigna mungo · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“by using Vigna mungo as test crop”

✓ located verbatim in the source · abstract, characters 325–358 · show in text ↑
e6

“16S rRNA sequencing revealed Rhizobium sp., ‘Stenotrophomonas rhizophila’ yielding most efficient outcomes followed by Bacillus subtilis, and Bradyrhizobium japonicum”

✓ located verbatim in the source · abstract, characters 643–809 · show in text ↑

Bradyrhizobium japonicum · yield · Vigna mungo (black gram)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

biochar · quality: protein · Vigna mungo (black gram)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

biochar · yield · Vigna mungo (black gram)Results agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.