Paper · 2025 · Journal of Animal Science

PSVIII-24 Bacterial and fungal communities during ensiling and aerobic exposure of whole corn silage treated with a bacterial inoculant or a propionic acid-based additive.

Michael Undi; Samat Amat; Godson Aryee; et al.

7
claims
14
verbatim quotes
0
citations
1
research areas
✓
Open access

Abstract

Abstract This study investigated the effect of silage additives on a) silage composition and fermentation characteristics, and b) bacterial and fungal communities during ensiling and aerobic exposure. Whole plant corn was harvested at 33% DM and ensiled in vacuum-sealed laboratory silos following treatment with a) deionized water (control; CON), b) a bacterial inoculant, LB+ Supersile® (Strong Microbials Inc., Milwaukee WI; INOC), applied to supply 1 x 105 cfu/g of fresh forage, c) a propionic acid-based additive, Silage SAVOR® Plus (KEMIN Industries, Des Moines, Iowa; PRE), applied at 0.1% of fresh forage, and d) additive plus inoculant (PRE+INOC) applied at the same rates as INOC and PRE. Silage was sampled twice; at silo opening after 180 d of ensiling and 21 d after silo opening. Silage composition and fermentation characteristics were measured at silo opening. For bacterial and fungal communities, we considered the effect of silage additives on two sampling dates in a 4 x 2 factorial arrangement. Bacterial and fungal communities were characterized using 16S rRNA gene amplicon and ITS sequencing, respectively. Treatment of silage with PRE and PRE+INOC increased CP, WSC, and lignin concentration relative to INOC. Main differences in fermentation characteristics among treatments were a greater pH, greater ammonia-N concentration, and lower total acids concentration in PRE and PRE+INOC-treated silages. Firmicutes and Proteobacteria were predominant bacterial phyla during ensiling and aerobic exposure. Firmicutes abundance was not impacted by silage additives but Proteobacteria abundance was reduced (P = 0.057) by PRE and PRE+INOC treatment.

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Claims 7

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Treatment with PRE and PRE+INOC increased CP, WSC and lignin concentration of corn silage relative to INOC.

Treatment
PRE and PRE+INOCe1
Outcome
CP, WSC, and lignin concentratione1
Direction
increasede1
Comparator
INOCe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Whole plant corn was harvested at 33% DM”

✓ located verbatim in the source · abstract, characters 201–241 · show in text ↑
e1

“Treatment of silage with PRE and PRE+INOC increased CP, WSC, and lignin concentration relative to INOC”

✓ located verbatim in the source · abstract, characters 1132–1234 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Silage treated with PRE and PRE+INOC had a greater pH.

Treatment
PRE and PRE+INOC-treated silagese2
Outcome
pHe2
Direction
greatere2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Whole plant corn was harvested at 33% DM”

✓ located verbatim in the source · abstract, characters 201–241 · show in text ↑
e2

“Main differences in fermentation characteristics among treatments were a greater pH, greater ammonia-N concentration, and lower total acids concentration in PRE and PRE+INOC-treated silages”

✓ located verbatim in the source · abstract, characters 1236–1425 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Silage treated with PRE and PRE+INOC had greater ammonia-N concentration.

Treatment
PRE and PRE+INOC-treated silagese2
Outcome
ammonia-N concentratione2
Direction
greatere2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Whole plant corn was harvested at 33% DM”

✓ located verbatim in the source · abstract, characters 201–241 · show in text ↑
e2

“Main differences in fermentation characteristics among treatments were a greater pH, greater ammonia-N concentration, and lower total acids concentration in PRE and PRE+INOC-treated silages”

✓ located verbatim in the source · abstract, characters 1236–1425 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Silage treated with PRE and PRE+INOC had lower total acids concentration.

Treatment
PRE and PRE+INOC-treated silagese2
Outcome
total acids concentratione2
Direction
lowere2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Whole plant corn was harvested at 33% DM”

✓ located verbatim in the source · abstract, characters 201–241 · show in text ↑
e2

“Main differences in fermentation characteristics among treatments were a greater pH, greater ammonia-N concentration, and lower total acids concentration in PRE and PRE+INOC-treated silages”

✓ located verbatim in the source · abstract, characters 1236–1425 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Proteobacteria abundance was reduced by PRE and PRE+INOC treatment (P = 0.057).

Treatment
PRE and PRE+INOC treatmente3
Outcome
Proteobacteria abundancee3
Direction
reducede3
Value
P = 0.057e3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Whole plant corn was harvested at 33% DM”

✓ located verbatim in the source · abstract, characters 201–241 · show in text ↑
e3

“Firmicutes abundance was not impacted by silage additives but Proteobacteria abundance was reduced (P = 0.057) by PRE and PRE+INOC treatment”

✓ located verbatim in the source · abstract, characters 1528–1668 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Firmicutes abundance was not impacted by silage additives.

Treatment
silage additivese3
Outcome
Firmicutes abundancee3
Direction
not impactede3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Whole plant corn was harvested at 33% DM”

✓ located verbatim in the source · abstract, characters 201–241 · show in text ↑
e3

“Firmicutes abundance was not impacted by silage additives but Proteobacteria abundance was reduced (P = 0.057) by PRE and PRE+INOC treatment”

✓ located verbatim in the source · abstract, characters 1528–1668 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Firmicutes and Proteobacteria were the predominant bacterial phyla during ensiling and aerobic exposure.

Outcome
predominant bacterial phylae4
Qualifier
Firmicutes and Proteobacteriae4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Whole plant corn was harvested at 33% DM”

✓ located verbatim in the source · abstract, characters 201–241 · show in text ↑
e4

“Firmicutes and Proteobacteria were predominant bacterial phyla during ensiling and aerobic exposure”

✓ located verbatim in the source · abstract, characters 1427–1526 · show in text ↑

processing · pest · Zea mays (maize)Positive and negative results

16 papers: 13 positive, 3 negative or no effect

2 papers in the same direction as this paper · 13 in the opposite direction

  1. positive
  2. positive
  3. positive

12 papers more in this group

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.