Paper · 2025 · Plant Science Today

A case study on three cultivars and optimization of LD50 dose of gamma irradiation for inducing variability in pomegranate for horticultural traits and bacterial blight resistance

J Munmun; D Rajesh; S Nisha; et al.

4
claims
8
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by

Abstract

Effective induced mutation breeding entails determining the ideal radiation dosage by examining how it influences the growth attributes of the crop. For evaluating the impact of different doses of gamma radiation on pomegranate (Punica granatum), three cultivars viz., Kandhari Kabuli, Bhagwa and Daru were treated with physical mutagen (gamma rays) at doses of 0, 6, 9, 12, 15, 18, 21, 24 kR to induce variability. The observations were recorded at different time intervals after sowing in the polyhouse and after transplanting in field conditions (20, 30 and 40 days after sowing and 70 days after transplanting, subsequently). The germination percentage, survival percentage and LD50 doses were calculated for all three cultivars. Mutant seedlings from different concentrations of gamma rays were evaluated for chlorophyll content of leaves and molecular characteristics. The LD50 doses for CVS. Kandhari Kabuli, Bhagwa and Daru were 15.26 kR, 15.08 kR and 13.74 kR, respectively. Statistical analysis revealed significant dose-dependent reductions in germination and survival rates (p < 0.05), with LD₅₀ values determined to be 15.26 kR for Kandhari Kabuli, 15.08 kR for Bhagwa and 13.74 kR for Daru. These results demonstrate scientifically important variability among cultivars in their sensitivity to gamma radiation, providing critical reference points for optimizing mutagenic doses in pomegranate breeding programs.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

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Claims 4

Observationobservedoriginal resultmeasured

Claim · model-written summary

The LD50 dose of Kandhari Kabuli pomegranate is 15.26 kR of gamma radiation.

Taxon
pomegranatePunica granatume1
Material
Kandhari Kabulie5
Outcome
LD50 dosese5
Value
15.26 kRe5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“For evaluating the impact of different doses of gamma radiation on pomegranate (Punica granatum), three cultivars viz., Kandhari Kabuli, Bhagwa and Daru were treated with physical mutagen (gamma rays) at doses of 0, 6, 9, 12, 15, 18, 21, 24 kR to induce variability.”

✓ located verbatim in the source · abstract, characters 149–415 · show in text ↑
e5

“The LD50 doses for CVS. Kandhari Kabuli, Bhagwa and Daru were 15.26 kR, 15.08 kR and 13.74 kR, respectively.”

✓ located verbatim in the source · abstract, characters 875–983 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The LD50 dose of Bhagwa pomegranate is 15.08 kR of gamma radiation.

Taxon
pomegranatePunica granatume1
Material
Bhagwae5
Outcome
LD50 dosese5
Value
15.08 kRe5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“For evaluating the impact of different doses of gamma radiation on pomegranate (Punica granatum), three cultivars viz., Kandhari Kabuli, Bhagwa and Daru were treated with physical mutagen (gamma rays) at doses of 0, 6, 9, 12, 15, 18, 21, 24 kR to induce variability.”

✓ located verbatim in the source · abstract, characters 149–415 · show in text ↑
e5

“The LD50 doses for CVS. Kandhari Kabuli, Bhagwa and Daru were 15.26 kR, 15.08 kR and 13.74 kR, respectively.”

✓ located verbatim in the source · abstract, characters 875–983 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The LD50 dose of Daru pomegranate is 13.74 kR of gamma radiation.

Taxon
pomegranatePunica granatume1
Material
Darue5
Outcome
LD50 dosese5
Value
13.74 kRe5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“For evaluating the impact of different doses of gamma radiation on pomegranate (Punica granatum), three cultivars viz., Kandhari Kabuli, Bhagwa and Daru were treated with physical mutagen (gamma rays) at doses of 0, 6, 9, 12, 15, 18, 21, 24 kR to induce variability.”

✓ located verbatim in the source · abstract, characters 149–415 · show in text ↑
e5

“The LD50 doses for CVS. Kandhari Kabuli, Bhagwa and Daru were 15.26 kR, 15.08 kR and 13.74 kR, respectively.”

✓ located verbatim in the source · abstract, characters 875–983 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Gamma radiation significantly reduced germination and survival of pomegranate in a dose-dependent manner.

Taxon
pomegranatePunica granatume1
Treatment
gamma radiatione1
Outcome
germination and survival ratese6
Direction
dose-dependent reductionse6
Qualifier
p < 0.05e6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“For evaluating the impact of different doses of gamma radiation on pomegranate (Punica granatum), three cultivars viz., Kandhari Kabuli, Bhagwa and Daru were treated with physical mutagen (gamma rays) at doses of 0, 6, 9, 12, 15, 18, 21, 24 kR to induce variability.”

✓ located verbatim in the source · abstract, characters 149–415 · show in text ↑
e6

“Statistical analysis revealed significant dose-dependent reductions in germination and survival rates (p < 0.05)”

✓ located verbatim in the source · abstract, characters 984–1096 · show in text ↑

radiation · growth · Punica granatum (pomegranate)Positive and negative results

3 papers: 1 positive, 2 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

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  2. negative
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.