Abstract
Aim. To investigate the physiological and biochemical characteristics of the seed generation (T4) of genetically modified wheat (Triticum aestivum L.) with a double-stranded RNA suppressor of the proline dehydrogenase (pdh) gene under osmotic stress. Methods. Proline dehydrogenase (PDH) activity assay, free L-proline (Pro) content; carbohydrates and protein. Results. The activity of proline dehydrogenase, the content of proline, proteins and carbohydrates under conditions of normal irrigation, water deficit and irrigation restoration were determined. Conclusions. It has been shown that genetically modified plants with partial suppression of pdh demonstrate changes in the complex of physiological and biochemical parameters that contribute to increased osmototoxicity. They are characterized by reduced activity of proline dehydrogenase and increased levels of proline. During dehydration, the level of Pro in T4 plants increased and remained elevated in the first hours after rehydration, which contributed to maintaining carbohydrate balance. The sucrose/monosaccharide ratio in transgenic variants remained stable regardless of cultivation conditions. Analysis of protein content showed that under optimal hydration, its level in T4 plants and the original genotypes did not differ significantly. At the same time, in control plants under water stress conditions, an increase in protein content was observed, which may indicate activation of the synthesis of stress response proteins.
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Claims 8
Claim · model-written summary
Genetically modified wheat with partial pdh suppression showed reduced proline dehydrogenase activity.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- partial suppression of pdhe1
- Gene
- pdhe1
- Outcome
- activity of proline dehydrogenasee2
- Direction
- reducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genetically modified wheat (Triticum aestivum L.)”
e1“genetically modified plants with partial suppression of pdh demonstrate changes in the complex of physiological and biochemical parameters that contribute to increased osmototoxicity”
e2“They are characterized by reduced activity of proline dehydrogenase and increased levels of proline”
Claim · model-written summary
Genetically modified wheat with partial pdh suppression showed increased proline levels.
- Taxon
- wheatTriticum aestivume0
- Treatment
- partial suppression of pdhe1
- Outcome
- levels of prolinee2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genetically modified wheat (Triticum aestivum L.)”
e1“genetically modified plants with partial suppression of pdh demonstrate changes in the complex of physiological and biochemical parameters that contribute to increased osmototoxicity”
e2“They are characterized by reduced activity of proline dehydrogenase and increased levels of proline”
Claim · model-written summary
During dehydration, proline level in T4 wheat plants increased and stayed elevated in the first hours after rehydration.
- Taxon
- wheatTriticum aestivume0
- Material
- T4 plantse3
- Treatment
- dehydratione3
- Outcome
- the level of Proe3
- Direction
- increasede3
- Qualifier
- remained elevated in the first hours after rehydratione3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genetically modified wheat (Triticum aestivum L.)”
e3“During dehydration, the level of Pro in T4 plants increased and remained elevated in the first hours after rehydration, which contributed to maintaining carbohydrate balance”
Claim · model-written summary
The increase in proline in T4 wheat plants is associated with maintaining carbohydrate balance during dehydration.
- Taxon
- wheatTriticum aestivume0
- Compound
- Proe3
- Outcome
- maintaining carbohydrate balancee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genetically modified wheat (Triticum aestivum L.)”
e3“During dehydration, the level of Pro in T4 plants increased and remained elevated in the first hours after rehydration, which contributed to maintaining carbohydrate balance”
Claim · model-written summary
The sucrose to monosaccharide ratio in transgenic wheat remained stable regardless of cultivation conditions.
- Taxon
- wheatTriticum aestivume0
- Material
- transgenic variantse4
- Outcome
- sucrose/monosaccharide ratioe4
- Qualifier
- regardless of cultivation conditionse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genetically modified wheat (Triticum aestivum L.)”
e4“The sucrose/monosaccharide ratio in transgenic variants remained stable regardless of cultivation conditions”
Claim · model-written summary
Under optimal hydration, protein content in T4 wheat plants and the original genotypes did not differ significantly.
- Taxon
- wheatTriticum aestivume0
- Material
- T4 plantse5
- Treatment
- optimal hydratione5
- Outcome
- protein contente5
- Direction
- did not differe5
- Comparator
- the original genotypese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genetically modified wheat (Triticum aestivum L.)”
e5“Analysis of protein content showed that under optimal hydration, its level in T4 plants and the original genotypes did not differ significantly”
Claim · model-written summary
In control wheat plants under water stress, protein content increased.
- Taxon
- wheatTriticum aestivume0
- Material
- control plantse6
- Treatment
- water stress conditionse6
- Outcome
- protein contente6
- Direction
- an increasee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genetically modified wheat (Triticum aestivum L.)”
e6“in control plants under water stress conditions, an increase in protein content was observed, which may indicate activation of the synthesis of stress response proteins”
Claim · model-written summary
The protein increase under water stress may indicate activation of stress response protein synthesis in wheat.
- Taxon
- wheatTriticum aestivume0
- Outcome
- protein contente6
- Qualifier
- activation of the synthesis of stress response proteinse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genetically modified wheat (Triticum aestivum L.)”
e6“in control plants under water stress conditions, an increase in protein content was observed, which may indicate activation of the synthesis of stress response proteins”
The same questions in other papers
loss of function · physiology: osmolytes · Triticum aestivum (wheat)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.