Paper · 2025 · Research Journal of Biotechnology

Phytochemical Profiling, Antibacterial, Antioxidant and GC-MS Analysis of Evolvulus alsinoides Extracts: Insights into Therapeutic Potential

Ranganath Tummalagunta; Rahamat Khan; Kanmani Kannan; et al.

9
claims
18
verbatim quotes
0
citations
1
research areas
—
Not open access

Abstract

Evolvulus alsinoides (L.) L., a revered medicinal herb in Ayurveda, was investigated for its phytochemical composition, antibacterial and antioxidant properties using water, ethanol, ethyl acetate, petroleum ether and chloroform extracts. The results of qualitative phytochemical screening included terpenoids, alkaloids, flavonoids, phenols and tannins with ethanol extracts exhibiting the highest diversity (10/11 classes). Quantitative analysis of the ethanol extract showed significant phenolic (180 ± 0.87 μg/mg), flavonoid (46 ± 0.92 μg/mg) and tannin (26 ± 0.56 μg/mg) content. Antibacterial assays via agar well diffusion demonstrated ethanol extracts’ efficacy against Escherichia coli (13.5 ± 0.5 mm) and Staphylococcus aureus (14.5 ± 0.7 mm) at 150 μg/mL. Antioxidant activity, assessed by DPPH and ABTS assays, yielded IC₅₀ values of 0.234 mg/mL and 0.077 mg/mL indicating robust radical scavenging. A study using GC-MS revealed 36 chemicals, including n-hexadecanoic acid, caryophyllene and benzoic acid derivatives, corroborating bioactivity. These findings validate the plant’s traditional uses for neuroprotection and inflammation management, highlighting ethanol’s extraction efficiency and suggesting pharmaceutical potential.

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Claims 9

Observationobservedoriginal resultmeasured

Claim · model-written summary

Ethanol extracts had the highest phytochemical class diversity, at 10 of 11 classes.

Taxon
Evolvulus alsinoidesEvolvulus alsinoidest0
Material
ethanol extractse0
Outcome
highest diversitye0
Value
10/11 classese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Evolvulus alsinoides
Evolvulus alsinoides · TaxonJ.M.Garg · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The results of qualitative phytochemical screening included terpenoids, alkaloids, flavonoids, phenols and tannins with ethanol extracts exhibiting the highest diversity (10/11 classes).”

✓ located verbatim in the source · abstract, characters 239–425 · show in text ↑
t0

“Phytochemical Profiling, Antibacterial, Antioxidant and GC-MS Analysis of Evolvulus alsinoides Extracts: Insights into Therapeutic Potential”

✓ located verbatim in the source · title, characters 0–140 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

The ethanol extract contained phenolics at 180 ± 0.87 μg/mg.

Taxon
Evolvulus alsinoidesEvolvulus alsinoidest0
Outcome
phenolice0
Value
180 ± 0.87 μg/mge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Evolvulus alsinoides
Evolvulus alsinoides · TaxonJ.M.Garg · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Quantitative analysis of the ethanol extract showed significant phenolic (180 ± 0.87 μg/mg), flavonoid (46 ± 0.92 μg/mg) and tannin (26 ± 0.56 μg/mg) content.”

✓ located verbatim in the source · abstract, characters 426–584 · show in text ↑
t0

“Phytochemical Profiling, Antibacterial, Antioxidant and GC-MS Analysis of Evolvulus alsinoides Extracts: Insights into Therapeutic Potential”

✓ located verbatim in the source · title, characters 0–140 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

The ethanol extract contained flavonoids at 46 ± 0.92 μg/mg.

Taxon
Evolvulus alsinoidesEvolvulus alsinoidest0
Outcome
flavonoide0
Value
46 ± 0.92 μg/mge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Evolvulus alsinoides
Evolvulus alsinoides · TaxonJ.M.Garg · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Quantitative analysis of the ethanol extract showed significant phenolic (180 ± 0.87 μg/mg), flavonoid (46 ± 0.92 μg/mg) and tannin (26 ± 0.56 μg/mg) content.”

✓ located verbatim in the source · abstract, characters 426–584 · show in text ↑
t0

“Phytochemical Profiling, Antibacterial, Antioxidant and GC-MS Analysis of Evolvulus alsinoides Extracts: Insights into Therapeutic Potential”

✓ located verbatim in the source · title, characters 0–140 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

The ethanol extract contained tannins at 26 ± 0.56 μg/mg.

Taxon
Evolvulus alsinoidesEvolvulus alsinoidest0
Outcome
tannine0
Value
26 ± 0.56 μg/mge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Evolvulus alsinoides
Evolvulus alsinoides · TaxonJ.M.Garg · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Quantitative analysis of the ethanol extract showed significant phenolic (180 ± 0.87 μg/mg), flavonoid (46 ± 0.92 μg/mg) and tannin (26 ± 0.56 μg/mg) content.”

✓ located verbatim in the source · abstract, characters 426–584 · show in text ↑
t0

“Phytochemical Profiling, Antibacterial, Antioxidant and GC-MS Analysis of Evolvulus alsinoides Extracts: Insights into Therapeutic Potential”

✓ located verbatim in the source · title, characters 0–140 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

At 150 μg/mL, ethanol extracts inhibited E. coli with a 13.5 ± 0.5 mm zone.

Taxon
Evolvulus alsinoidesEvolvulus alsinoidest0
Outcome
efficacy against Escherichia colie0
Value
13.5 ± 0.5 mme0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Evolvulus alsinoides
Evolvulus alsinoides · TaxonJ.M.Garg · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Antibacterial assays via agar well diffusion demonstrated ethanol extracts’ efficacy against Escherichia coli (13.5 ± 0.5 mm) and Staphylococcus aureus (14.5 ± 0.7 mm) at 150 μg/mL.”

✓ located verbatim in the source · abstract, characters 585–766 · show in text ↑
t0

“Phytochemical Profiling, Antibacterial, Antioxidant and GC-MS Analysis of Evolvulus alsinoides Extracts: Insights into Therapeutic Potential”

✓ located verbatim in the source · title, characters 0–140 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

At 150 μg/mL, ethanol extracts inhibited S. aureus with a 14.5 ± 0.7 mm zone.

Taxon
Evolvulus alsinoidesEvolvulus alsinoidest0
Outcome
Staphylococcus aureuse0
Value
14.5 ± 0.7 mme0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Evolvulus alsinoides
Evolvulus alsinoides · TaxonJ.M.Garg · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Antibacterial assays via agar well diffusion demonstrated ethanol extracts’ efficacy against Escherichia coli (13.5 ± 0.5 mm) and Staphylococcus aureus (14.5 ± 0.7 mm) at 150 μg/mL.”

✓ located verbatim in the source · abstract, characters 585–766 · show in text ↑
t0

“Phytochemical Profiling, Antibacterial, Antioxidant and GC-MS Analysis of Evolvulus alsinoides Extracts: Insights into Therapeutic Potential”

✓ located verbatim in the source · title, characters 0–140 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

DPPH antioxidant activity had an IC₅₀ of 0.234 mg/mL.

Taxon
Evolvulus alsinoidesEvolvulus alsinoidest0
Outcome
IC₅₀e0
Value
0.234 mg/mLe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Evolvulus alsinoides
Evolvulus alsinoides · TaxonJ.M.Garg · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Antioxidant activity, assessed by DPPH and ABTS assays, yielded IC₅₀ values of 0.234 mg/mL and 0.077 mg/mL indicating robust radical scavenging.”

✓ located verbatim in the source · abstract, characters 767–911 · show in text ↑
t0

“Phytochemical Profiling, Antibacterial, Antioxidant and GC-MS Analysis of Evolvulus alsinoides Extracts: Insights into Therapeutic Potential”

✓ located verbatim in the source · title, characters 0–140 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

ABTS antioxidant activity had an IC₅₀ of 0.077 mg/mL.

Taxon
Evolvulus alsinoidesEvolvulus alsinoidest0
Outcome
IC₅₀e0
Value
0.077 mg/mLe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Evolvulus alsinoides
Evolvulus alsinoides · TaxonJ.M.Garg · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Antioxidant activity, assessed by DPPH and ABTS assays, yielded IC₅₀ values of 0.234 mg/mL and 0.077 mg/mL indicating robust radical scavenging.”

✓ located verbatim in the source · abstract, characters 767–911 · show in text ↑
t0

“Phytochemical Profiling, Antibacterial, Antioxidant and GC-MS Analysis of Evolvulus alsinoides Extracts: Insights into Therapeutic Potential”

✓ located verbatim in the source · title, characters 0–140 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

GC-MS identified 36 chemicals, including n-hexadecanoic acid and caryophyllene.

Taxon
Evolvulus alsinoidesEvolvulus alsinoidest0
Outcome
chemicalse0
Value
36e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Evolvulus alsinoides
Evolvulus alsinoides · TaxonJ.M.Garg · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A study using GC-MS revealed 36 chemicals, including n-hexadecanoic acid, caryophyllene and benzoic acid derivatives, corroborating bioactivity.”

✓ located verbatim in the source · abstract, characters 912–1056 · show in text ↑
t0

“Phytochemical Profiling, Antibacterial, Antioxidant and GC-MS Analysis of Evolvulus alsinoides Extracts: Insights into Therapeutic Potential”

✓ located verbatim in the source · title, characters 0–140 · show in text ↑