Abstract
The aim of the study was to optimize the stages of garden strawberry micropropagation. To introduce the Elizaveta 2 variety and the K 10 selection line into in vitro culture, the growth points of the firstorder runners were used as explants using the following sterilization scheme: soap solution; running water; 70% ethanol; 12 % H 2 O 2 . At the stage of micropropagation itself, Murashige-Skoog medium was used with a sucrose concentration of 0, 10, 20, 30 g/l, supplemented with 0.5 mg/l 6-benzylaminopurine (BAP). The final stage of the work included determining the ability of the regenerates to adapt to ex vitro conditions. Vermiculite was used as a substrate. The experiment was repeated three times. The number of roots and microplants from the explant, as well as their viability at the adaptation stage, were recorded. The Elizaveta 2 variety and the K 10 line did not have statistically significant differences in the increase in the number of microshoots. The Elizaveta 2 variety showed a tendency to decrease by 2.03 pcs. (LSD05 = 4.09) in the variant with the addition of 30 g/l sucrose, and the number of roots statistically significantly exceeded the value of the indicator on the control medium (20 g/l sucrose; LSD 05 = 2.64) by 3.23 pcs. On the nutrient medium with the addition of 10 g/l sucrose, the number of roots decreased by 3.77 pcs., and on the medium without sucrose – by 8.38 pcs. The K10 selection line did not form roots in the variant without the addition of sucrose, and no statistically significant deviations were observed in the other variants. Under ex vitro conditions, the micro plants were characterized by 88.8–100 % viability after in vitro cultivation on variants with 20–30 g/l sucrose. Adapted micro plants, cultivated in vitro on a diet containing 30 g/l of sucrose, have a powerful root system (Elizaveta 2 by 6.42 pcs. more, line K 10 by 4.23 pcs., LSD 05 = 4.86), compared to the indicators in the control variant.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Elizaveta 2 and K 10 did not significantly differ in microshoot increase.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassat0 - Material
- Elizaveta 2e0
- Outcome
- increase in the number of microshootse0
- Direction
- did not have statistically significant differencese0
- Comparator
- K 10e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“The Elizaveta 2 variety and the K 10 line did not have statistically significant differences in the increase in the number of microshoots”
t0“strawberry regenerants”
Claim · model-written summary
Elizaveta 2 microshoot number tended to decrease by 2.03 at 30 g/l sucrose.
- Taxon
- strawberryFragaria ×ananassat0
- Material
- Elizaveta 2e0
- Treatment
- 30 g/l sucrosee0
- Outcome
- tendency to decreasee0
- Direction
- decreasee0
- Value
- 2.03 pcs.e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Elizaveta 2 variety showed a tendency to decrease by 2.03 pcs. (LSD05 = 4.09) in the variant with the addition of 30 g/l sucrose”
t0“strawberry regenerants”
Claim · model-written summary
Elizaveta 2 root number exceeded control by 3.23 at 30 g/l sucrose.
- Taxon
- strawberryFragaria ×ananassat0
- Material
- Elizaveta 2e1
- Treatment
- 30 g/l sucrosee2
- Outcome
- number of rootse0
- Direction
- exceedede0
- Value
- 3.23 pcs.e0
- Comparator
- control mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the number of roots statistically significantly exceeded the value of the indicator on the control medium (20 g/l sucrose; LSD 05 = 2.64) by 3.23 pcs.”
t0“strawberry regenerants”
e1“The Elizaveta 2 variety and the K 10 line did not have statistically significant differences in the increase in the number of microshoots”
e2“The Elizaveta 2 variety showed a tendency to decrease by 2.03 pcs. (LSD05 = 4.09) in the variant with the addition of 30 g/l sucrose”
Claim · model-written summary
At 10 g/l sucrose, root number decreased by 3.77.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- 10 g/l sucrosee0
- Outcome
- number of rootse0
- Direction
- decreasede0
- Value
- 3.77 pcs.e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On the nutrient medium with the addition of 10 g/l sucrose, the number of roots decreased by 3.77 pcs.”
t0“strawberry regenerants”
Claim · model-written summary
Without sucrose, root number decreased by 8.38.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- without sucrosee0
- Outcome
- number of rootse1
- Value
- 8.38 pcs.e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“on the medium without sucrose – by 8.38 pcs.”
t0“strawberry regenerants”
e1“the number of roots statistically significantly exceeded the value of the indicator on the control medium (20 g/l sucrose; LSD 05 = 2.64) by 3.23 pcs.”
Claim · model-written summary
K10 formed no roots without sucrose.
- Taxon
- strawberryFragaria ×ananassat0
- Material
- K10e0
- Treatment
- without the addition of sucrosee0
- Outcome
- did not form rootse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The K10 selection line did not form roots in the variant without the addition of sucrose”
t0“strawberry regenerants”
Claim · model-written summary
K10 showed no significant deviations under other sucrose variants.
- Taxon
- strawberryFragaria ×ananassat0
- Material
- K10e1
- Outcome
- statistically significant deviationse0
- Direction
- noe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no statistically significant deviations were observed in the other variants”
t0“strawberry regenerants”
e1“The K10 selection line did not form roots in the variant without the addition of sucrose”
Claim · model-written summary
Microplants had 88.8–100% ex vitro viability after 20–30 g/l sucrose.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- 20–30 g/l sucrosee0
- Outcome
- viabilitye0
- Value
- 88.8–100 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under ex vitro conditions, the micro plants were characterized by 88.8–100 % viability after in vitro cultivation on variants with 20–30 g/l sucrose”
t0“strawberry regenerants”
Claim · model-written summary
Elizaveta 2 adapted plants had 6.42 more roots after 30 g/l sucrose than controls.
- Taxon
- strawberryFragaria ×ananassat0
- Material
- Elizaveta 2e0
- Treatment
- 30 g/l of sucrosee0
- Outcome
- root systeme0
- Direction
- moree0
- Value
- 6.42 pcs.e0
- Comparator
- control variante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Adapted micro plants, cultivated in vitro on a diet containing 30 g/l of sucrose, have a powerful root system (Elizaveta 2 by 6.42 pcs. more, line K 10 by 4.23 pcs., LSD 05 = 4.86), compared to the indicators in the control variant”
t0“strawberry regenerants”
Claim · model-written summary
K 10 adapted plants had 4.23 more roots after 30 g/l sucrose than controls.
- Taxon
- strawberryFragaria ×ananassat0
- Material
- K 10e0
- Treatment
- 30 g/l of sucrosee0
- Outcome
- root systeme0
- Direction
- moree0
- Value
- 4.23 pcs.e0
- Comparator
- control variante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Adapted micro plants, cultivated in vitro on a diet containing 30 g/l of sucrose, have a powerful root system (Elizaveta 2 by 6.42 pcs. more, line K 10 by 4.23 pcs., LSD 05 = 4.86), compared to the indicators in the control variant”
t0“strawberry regenerants”
The same questions in other papers
sucrose · growth · Fragaria x ananassa (strawberry)Positive and negative results
9 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.