Abstract
Hexavalent chromium [Cr(VI)] is a highly toxic heavy metal that adversely affects plant growth and development. Non-structural carbohydrates (NSCs) serve as dynamic metabolic buffers under environmental stress, balancing growth and detoxification demands. This study elucidates how exogenous citric acid (CA) alters subcellar distribution of Cr(VI) and reprograms NSC allocation to alleviate Cr(VI) toxicity in Oryza sativa through integrated biochemical and transcriptomic analyses. CA application significantly enhanced biomass growth in Cr(VI)-stressed seedlings, with Cr(VI) redistribution across subcellular compartments and NSC reconfiguration. Tissue-specific transcriptomic shifts revealed CA-mediated modulation of NSC metabolic genes, transporters, and signaling components. Genome-scale metabolic network modeling identified OsNIN4 and OsTPP3 as predicted regulatory nodes to mediate a dynamic equilibrium between NSC partitioning and Cr(VI) detoxification in roots of Cr(VI)-treated rice seedlings supplied with exogenous CA. OsNIN4 suppressed sucrose synthesis to favor nitrogen-based defenses, while OsTPP3 enhanced fructose retention via trehalose-OsSnRK signaling network from “CA + Cr(VI)” treatments. Divergent expression patterns of other NSC-associated genes revealed the multifaceted regulatory mechanism governing NSC metabolism, translocation, and stress-responsive signaling. Exogenous CA application improved the growth of Cr(VI)-treated rice seedlings. The integrated analysis of genome-scale metabolic network indicated OsNIN4 and OsTPP3 as predicted regulatory nodes for optimizing NSC flux during CA-mediated Cr(VI) detoxification in rice plants.
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Claims 9
Claim · model-written summary
Citric acid significantly increased biomass growth of chromium-stressed rice seedlings.
- Taxon
- rice
Oryza sativat0 - Treatment
- CA applicatione0
- Outcome
- biomass growthe0
- Direction
- enhancede0
- Qualifier
- Cr(VI)-stressed seedlingse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CA application significantly enhanced biomass growth in Cr(VI)-stressed seedlings”
t0“rice”
Claim · model-written summary
Citric acid redistributed chromium across subcellular compartments.
- Taxon
- riceOryza sativat0
- Outcome
- Cr(VI) redistribution across subcellular compartmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with Cr(VI) redistribution across subcellular compartments and NSC reconfiguration”
t0“rice”
Claim · model-written summary
Citric acid reconfigured nonstructural carbohydrates.
- Taxon
- riceOryza sativat0
- Outcome
- NSC reconfiguratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with Cr(VI) redistribution across subcellular compartments and NSC reconfiguration”
t0“rice”
Claim · model-written summary
Transcriptomic shifts showed modulation of carbohydrate metabolic genes, transporters and signaling components.
- Taxon
- riceOryza sativat0
- Treatment
- CA-mediatede0
- Outcome
- modulation of NSC metabolic genes, transporters, and signaling componentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tissue-specific transcriptomic shifts revealed CA-mediated modulation of NSC metabolic genes, transporters, and signaling components”
t0“rice”
Claim · model-written summary
Network modeling predicted OsNIN4 as a regulatory node of carbohydrate partitioning and chromium detoxification.
- Taxon
- riceOryza sativae0
- Gene
- OsNIN4e0
- Outcome
- predicted regulatory nodese0
- Qualifier
- NSC partitioning and Cr(VI) detoxificatione0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genome-scale metabolic network modeling identified OsNIN4 and OsTPP3 as predicted regulatory nodes to mediate a dynamic equilibrium between NSC partitioning and Cr(VI) detoxification in roots of Cr(VI)-treated rice seedlings supplied with exogenous CA”
Claim · model-written summary
Network modeling predicted OsTPP3 as a regulatory node of carbohydrate partitioning and chromium detoxification.
- Taxon
- riceOryza sativae0
- Gene
- OsTPP3e0
- Outcome
- predicted regulatory nodese0
- Qualifier
- NSC partitioning and Cr(VI) detoxificatione0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genome-scale metabolic network modeling identified OsNIN4 and OsTPP3 as predicted regulatory nodes to mediate a dynamic equilibrium between NSC partitioning and Cr(VI) detoxification in roots of Cr(VI)-treated rice seedlings supplied with exogenous CA”
Claim · model-written summary
OsNIN4 suppressed sucrose synthesis to favor nitrogen-based defenses.
- Taxon
- riceOryza sativat0
- Gene
- OsNIN4e0
- Outcome
- sucrose synthesise0
- Direction
- suppressede0
- Qualifier
- nitrogen-based defensese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsNIN4 suppressed sucrose synthesis to favor nitrogen-based defenses”
t0“rice”
Claim · model-written summary
OsTPP3 enhanced fructose retention through trehalose-OsSnRK signaling under CA plus chromium treatment.
- Taxon
- riceOryza sativat0
- Treatment
- CA + Cr(VI)e0
- Gene
- OsTPP3e0
- Outcome
- fructose retentione0
- Direction
- enhancede0
- Qualifier
- trehalose-OsSnRK signaling networke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsTPP3 enhanced fructose retention via trehalose-OsSnRK signaling network from “CA + Cr(VI)” treatments”
t0“rice”
Claim · model-written summary
Other carbohydrate-associated genes showed divergent expression patterns.
- Taxon
- riceOryza sativat0
- Gene
- NSC-associated genese0
- Outcome
- Divergent expression patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Divergent expression patterns of other NSC-associated genes revealed the multifaceted regulatory mechanism governing NSC metabolism, translocation, and stress-responsive signaling”
t0“rice”
The same questions in other papers
citric acid · growth · Oryza sativa (rice)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.