Abstract
Radish (Raphanus sativus), a commonly grown root vegetable prized for its nutrition and culinary use, is particularly vulnerable to lead (Pb) stress, which mainly results in Pb accumulation in the roots. However, the molecular mechanisms underlying Pb accumulation in radish remain largely unknown. In this study, we investigated the role of BASIC PENTACYSTEINE (BPC) genes in radish’s response to Pb stress. Phylogenetic analysis revealed that radish contains 10 BPC genes, which are distinctly clustered in Cluster III. Expression analysis revealed that, except for RsBPC2, RsBPC4, and RsBPC7, the expression of most RsBPC genes was significantly altered under Pb stress. Notably, the expression of RsBPC5 gradually decreased with prolonged Pb exposure. Subcellular localization analysis confirmed that RsBPC5 is localized in the nucleus and acts as a transcriptional repressor. Functional assays demonstrated that transient overexpression of RsBPC5 enhanced the tolerance of radish plants to Pb stress via reducing Pb accumulation and activating the antioxidant defense system. Collectively, our findings suggest that RsBPC5 plays a key role in radish’s response to Pb stress, potentially improving Pb tolerance by modulating Pb uptake and strengthening antioxidant defense mechanisms.
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Claims 8
Claim · model-written summary
Radish contains 10 BPC genes clustered in Cluster III.
- Taxon
- radish
Raphanus sativuse0 - Outcome
- BPC genese0
- Value
- 10 BPC genese0
- Qualifier
- Cluster IIIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“radish contains 10 BPC genes, which are distinctly clustered in Cluster III”
Claim · model-written summary
Most RsBPC genes except RsBPC2, RsBPC4, and RsBPC7 changed expression under Pb stress.
- Taxon
- RadishRaphanus sativuse1
- Treatment
- Pb stresse0
- Gene
- RsBPC genese0
- Outcome
- expression of most RsBPC genese0
- Qualifier
- except for RsBPC2, RsBPC4, and RsBPC7e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“except for RsBPC2, RsBPC4, and RsBPC7, the expression of most RsBPC genes was significantly altered under Pb stress”
e1“Radish (Raphanus sativus)”
Claim · model-written summary
RsBPC5 expression decreased with prolonged Pb exposure.
- Taxon
- RadishRaphanus sativuse1
- Treatment
- prolonged Pb exposuree0
- Gene
- RsBPC5e0
- Outcome
- expression of RsBPC5e0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of RsBPC5 gradually decreased with prolonged Pb exposure”
e1“Radish (Raphanus sativus)”
Claim · model-written summary
RsBPC5 localizes to the nucleus.
- Taxon
- RadishRaphanus sativuse1
- Gene
- RsBPC5e0
- Outcome
- localized in the nucleuse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RsBPC5 is localized in the nucleus and acts as a transcriptional repressor”
e1“Radish (Raphanus sativus)”
Claim · model-written summary
RsBPC5 acts as a transcriptional repressor.
- Taxon
- RadishRaphanus sativuse1
- Gene
- RsBPC5e0
- Outcome
- transcriptional repressore0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RsBPC5 is localized in the nucleus and acts as a transcriptional repressor”
e1“Radish (Raphanus sativus)”
Claim · model-written summary
Transient RsBPC5 overexpression enhanced Pb stress tolerance.
- Taxon
- radishRaphanus sativuse0
- Treatment
- transient overexpression of RsBPC5e0
- Gene
- RsBPC5e0
- Outcome
- tolerance of radish plants to Pb stresse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient overexpression of RsBPC5 enhanced the tolerance of radish plants to Pb stress”
Claim · model-written summary
Transient RsBPC5 overexpression reduced Pb accumulation.
- Taxon
- radishRaphanus sativuse0
- Treatment
- transient overexpression of RsBPC5e0
- Gene
- RsBPC5e0
- Outcome
- Pb accumulatione0
- Direction
- reducinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient overexpression of RsBPC5 enhanced the tolerance of radish plants to Pb stress via reducing Pb accumulation”
Claim · model-written summary
Transient RsBPC5 overexpression activated antioxidant defenses.
- Taxon
- radishRaphanus sativuse0
- Treatment
- transient overexpression of RsBPC5e0
- Gene
- RsBPC5e0
- Outcome
- antioxidant defense systeme0
- Direction
- activatinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient overexpression of RsBPC5 enhanced the tolerance of radish plants to Pb stress via reducing Pb accumulation and activating the antioxidant defense system”
The same questions in other papers
lead atom · molecular: expression · Raphanus sativus (radish)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.