Abstract
Cyclic lipopeptides (CLPs) produced by Bacillus species have been reported to have several antimicrobial effects against plant pathogens through different mechanisms such as destabilisation of plasma membrane and loss of electrolytes. The present study focused on the production, extraction, identification of CLPs produced by B. velezensis RC218 and evaluation of their activity against the phytopathogen Fusarium graminearum. Mass spectroscopy confirmed the presence of these lipopeptides, with several carbon-length variants in each family. Other antimicrobial molecules such as macrolactin, dificidin and bacilysin were also detected. In vitro experiments showed a significant reduction in the mycelial radial growth (up to 84%) of three F. graminearum sensu stricto strains isolated from a Fusarium head blight (FHB) epidemic. Greenhouse experiments were carried out on BioINTA 1005 (FHB-susceptible wheat) and the tolerant variety Cronox. Application of the pure extract to flowering heads, prior to the pathogen inoculation, resulted in reduced disease incidence and severity in both cultivars. The biocontrol effect was more pronounced in the susceptible variety (78.5% reduction in incidence and 82.5% in severity) compared to the tolerant Cronox (69.3% and 66.3% reductions, respectively). Given that metabolites production by bacteria at the in planta level can be altered by the plant-environment interaction, applying the purified lipopeptide extract from B. velezensis RC218 as a preventive against FHB emerges as a promising method to control pathogens.
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Claims 8
Claim · model-written summary
Mass spectroscopy confirmed the presence of cyclic lipopeptides produced by Bacillus velezensis RC218, with several carbon-length variants in each family.
- Taxon
- Bacillus velezensis RC218
Bacillus velezensist0 - Outcome
- presence of these lipopeptidese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Bacillus velezensis RC218”
e0“Mass spectroscopy confirmed the presence of these lipopeptides, with several carbon-length variants in each family”
Claim · model-written summary
Macrolactin, dificidin and bacilysin were also detected as other antimicrobial molecules from B. velezensis RC218.
- Taxon
- Bacillus velezensis RC218Bacillus velezensist0
- Outcome
- macrolactin, dificidin and bacilysine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Bacillus velezensis RC218”
e0“Other antimicrobial molecules such as macrolactin, dificidin and bacilysin were also detected”
Claim · model-written summary
In vitro, the lipopeptides significantly reduced mycelial radial growth of three F. graminearum sensu stricto strains by up to 84%.
- Taxon
- F. graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume0 - Treatment
- CLPse1
- Interactor
- F. graminearumFusarium graminearume0pathogen
- Second interactor
- B. velezensis RC218Bacillus velezensise1biocontrol_agent
- Outcome
- mycelial radial growthe0
- Direction
- reductione0
- Value
- up to 84%e0
- Setting
- In vitroe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e0“In vitro experiments showed a significant reduction in the mycelial radial growth (up to 84%) of three F. graminearum sensu stricto strains”
e1“CLPs produced by B. velezensis RC218”
Claim · model-written summary
In the greenhouse, the pure extract applied before pathogen inoculation reduced Fusarium head blight incidence by 78.5% in the susceptible wheat BioINTA 1005.
- Taxon
- wheat
Triticum aestivume0 - Material
- BioINTA 1005e0
- Treatment
- pure extracte1
- Interactor
- Fusarium graminearumFusarium graminearume3pathogen
- Outcome
- incidencee2
- Direction
- reductione2
- Value
- 78.5%e2
- Setting
- Greenhousee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Greenhouse experiments were carried out on BioINTA 1005 (FHB-susceptible wheat) and the tolerant variety Cronox”
e1“Application of the pure extract to flowering heads, prior to the pathogen inoculation”
e2“The biocontrol effect was more pronounced in the susceptible variety (78.5% reduction in incidence and 82.5% in severity) compared to the tolerant Cronox (69.3% and 66.3% reductions, respectively)”
e3“the phytopathogen Fusarium graminearum”
Claim · model-written summary
In the greenhouse, the pure extract reduced Fusarium head blight severity by 82.5% in the susceptible wheat BioINTA 1005.
- Taxon
- wheatTriticum aestivume0
- Material
- BioINTA 1005e0
- Treatment
- pure extracte1
- Interactor
- Fusarium graminearumFusarium graminearume3pathogen
- Outcome
- severitye2
- Direction
- reductione2
- Value
- 82.5%e2
- Setting
- Greenhousee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Greenhouse experiments were carried out on BioINTA 1005 (FHB-susceptible wheat) and the tolerant variety Cronox”
e1“Application of the pure extract to flowering heads, prior to the pathogen inoculation”
e2“The biocontrol effect was more pronounced in the susceptible variety (78.5% reduction in incidence and 82.5% in severity) compared to the tolerant Cronox (69.3% and 66.3% reductions, respectively)”
e3“the phytopathogen Fusarium graminearum”
Claim · model-written summary
In the greenhouse, the pure extract reduced Fusarium head blight incidence by 69.3% in the tolerant wheat variety Cronox.
- Taxon
- wheatTriticum aestivume0
- Material
- Cronoxe0
- Treatment
- pure extracte1
- Interactor
- Fusarium graminearumFusarium graminearume3pathogen
- Outcome
- incidencee2
- Direction
- reductione2
- Value
- 69.3%e2
- Setting
- Greenhousee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Greenhouse experiments were carried out on BioINTA 1005 (FHB-susceptible wheat) and the tolerant variety Cronox”
e1“Application of the pure extract to flowering heads, prior to the pathogen inoculation”
e2“The biocontrol effect was more pronounced in the susceptible variety (78.5% reduction in incidence and 82.5% in severity) compared to the tolerant Cronox (69.3% and 66.3% reductions, respectively)”
e3“the phytopathogen Fusarium graminearum”
Claim · model-written summary
In the greenhouse, the pure extract reduced Fusarium head blight severity by 66.3% in the tolerant wheat variety Cronox.
- Taxon
- wheatTriticum aestivume0
- Material
- Cronoxe0
- Treatment
- pure extracte1
- Interactor
- Fusarium graminearumFusarium graminearume3pathogen
- Outcome
- severitye2
- Direction
- reductione2
- Value
- 66.3%e2
- Setting
- Greenhousee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Greenhouse experiments were carried out on BioINTA 1005 (FHB-susceptible wheat) and the tolerant variety Cronox”
e1“Application of the pure extract to flowering heads, prior to the pathogen inoculation”
e2“The biocontrol effect was more pronounced in the susceptible variety (78.5% reduction in incidence and 82.5% in severity) compared to the tolerant Cronox (69.3% and 66.3% reductions, respectively)”
e3“the phytopathogen Fusarium graminearum”
Claim · model-written summary
The authors conclude that applying the purified lipopeptide extract from B. velezensis RC218 as a preventive against FHB is a promising method to control pathogens.
- Outcome
- preventive against FHBe0
Not reportedTaxon
Verbatim
e0“applying the purified lipopeptide extract from B. velezensis RC218 as a preventive against FHB emerges as a promising method to control pathogens”
The same questions in other papers
botanical · Fusarium graminearum · Triticum aestivum (wheat)Positive and negative results
10 papers in the same direction as this paper · 3 in the opposite direction
- no effect
- negative
- no effect
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.