Abstract
Introduction Bletilla striata (Orchidaceae) is a valuable traditional Chinese medicinal plant prized for its dried rhizomes. However, its cultivation faces significant challenges from leaf rust disease caused by Coleosporium bletiae , which causes substantial yield losses. Methods To investigate host resistance mechanisms, we compared rust-resistant and susceptible B. striata accessions through integrated transcriptomic and physiological analyses. Results and discussion Phenotypic observations revealed that while both resistant and susceptible plants developed rust spores by 2 days post-inoculation (dpi), the resistant accession exhibited a significantly slower progression of spore stack formation and lesion expansion on abaxial leaf surfaces over time. Integrated transcriptomic and physiological analyses revealed that the rust-resistant material of B. striata accessions exhibited faster and stronger defense responses to pathogen infection compared to susceptible plants. These responses were characterized by significant upregulation of DEGs associated with antioxidant defense systems, secondary metabolite biosynthesis, JA, SA, and BR signaling pathways, concurrent downregulation of DEGs involved in cell wall remodeling, and calcium-mediated signaling. Furthermore, rust pathogen inoculation triggered rapid physiological responses in resistant plants, including enhanced activity of defense-related enzymes (CAT, PAL, β-1,3-glucanase, and chitinase) and early accumulation of osmolytes (soluble sugars, soluble proteins, and proline). These coordinated molecular and biochemical responses effectively restricted pathogen colonization and spread. These findings delineate the molecular basis of rust resistance in B. striata , identifying key regulatory nodes in defense pathways that could be targeted through precision breeding or genetic engineering to develop durable resistance against C. bletilla .
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
Both resistant and susceptible Bletilla striata plants developed rust spores by 2 days post-inoculation.
- Taxon
- Bletilla striata
Bletilla striatat0 - Material
- resistant and susceptible plantse1
- Outcome
- developed rust sporese1
- Qualifier
- by 2 days post-inoculation (dpi)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptomics combined with physiological analysis provides insights into the mechanism of resistance to Coleosporium bletiae in Bletilla striata”
e1“Phenotypic observations revealed that while both resistant and susceptible plants developed rust spores by 2 days post-inoculation (dpi), the resistant accession exhibited a significantly slower progression of spore stack formation and lesion expansion on abaxial leaf surfaces over time”
Claim · model-written summary
The resistant Bletilla striata accession showed significantly slower progression of spore stack formation and lesion expansion than susceptible plants.
- Taxon
- Bletilla striataBletilla striatat0
- Material
- resistant accessione1
- Interactor
- Coleosporium bletiaeColeosporium bletiaee0pathogen
- Outcome
- progression of spore stack formation and lesion expansione1
- Direction
- slowere1
- Comparator
- susceptible plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptomics combined with physiological analysis provides insights into the mechanism of resistance to Coleosporium bletiae in Bletilla striata”
e0“leaf rust disease caused by Coleosporium bletiae”
e1“Phenotypic observations revealed that while both resistant and susceptible plants developed rust spores by 2 days post-inoculation (dpi), the resistant accession exhibited a significantly slower progression of spore stack formation and lesion expansion on abaxial leaf surfaces over time”
Claim · model-written summary
Rust-resistant Bletilla striata accessions showed faster and stronger defense responses to pathogen infection than susceptible plants.
- Taxon
- B. striataBletilla striatae2
- Material
- rust-resistant materiale2
- Treatment
- pathogen infectione2
- Outcome
- defense responsese2
- Direction
- faster and strongere2
- Comparator
- susceptible plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptomics combined with physiological analysis provides insights into the mechanism of resistance to Coleosporium bletiae in Bletilla striata”
e2“the rust-resistant material of B. striata accessions exhibited faster and stronger defense responses to pathogen infection compared to susceptible plants”
Claim · model-written summary
Rust-resistant Bletilla striata accessions showed significant upregulation of DEGs associated with antioxidant defense systems.
- Taxon
- Bletilla striataBletilla striatat0
- Material
- rust-resistant materiale2
- Outcome
- DEGs associated with antioxidant defense systemse3
- Direction
- upregulatione3
- Comparator
- susceptible plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptomics combined with physiological analysis provides insights into the mechanism of resistance to Coleosporium bletiae in Bletilla striata”
e2“the rust-resistant material of B. striata accessions exhibited faster and stronger defense responses to pathogen infection compared to susceptible plants”
e3“These responses were characterized by significant upregulation of DEGs associated with antioxidant defense systems, secondary metabolite biosynthesis, JA, SA, and BR signaling pathways, concurrent downregulation of DEGs involved in cell wall remodeling, and calcium-mediated signaling”
Claim · model-written summary
Rust-resistant Bletilla striata accessions showed significant upregulation of DEGs associated with secondary metabolite biosynthesis.
- Taxon
- Bletilla striataBletilla striatat0
- Material
- rust-resistant materiale2
- Outcome
- secondary metabolite biosynthesise3
- Direction
- upregulatione3
- Comparator
- susceptible plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptomics combined with physiological analysis provides insights into the mechanism of resistance to Coleosporium bletiae in Bletilla striata”
e2“the rust-resistant material of B. striata accessions exhibited faster and stronger defense responses to pathogen infection compared to susceptible plants”
e3“These responses were characterized by significant upregulation of DEGs associated with antioxidant defense systems, secondary metabolite biosynthesis, JA, SA, and BR signaling pathways, concurrent downregulation of DEGs involved in cell wall remodeling, and calcium-mediated signaling”
Claim · model-written summary
Rust-resistant Bletilla striata accessions showed significant upregulation of JA, SA and BR signaling pathway DEGs.
- Taxon
- Bletilla striataBletilla striatat0
- Material
- rust-resistant materiale2
- Outcome
- JA, SA, and BR signaling pathwayse3
- Direction
- upregulatione3
- Comparator
- susceptible plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptomics combined with physiological analysis provides insights into the mechanism of resistance to Coleosporium bletiae in Bletilla striata”
e2“the rust-resistant material of B. striata accessions exhibited faster and stronger defense responses to pathogen infection compared to susceptible plants”
e3“These responses were characterized by significant upregulation of DEGs associated with antioxidant defense systems, secondary metabolite biosynthesis, JA, SA, and BR signaling pathways, concurrent downregulation of DEGs involved in cell wall remodeling, and calcium-mediated signaling”
Claim · model-written summary
Rust-resistant Bletilla striata accessions showed downregulation of DEGs involved in cell wall remodeling.
- Taxon
- Bletilla striataBletilla striatat0
- Material
- rust-resistant materiale2
- Outcome
- DEGs involved in cell wall remodelinge3
- Direction
- downregulatione3
- Comparator
- susceptible plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptomics combined with physiological analysis provides insights into the mechanism of resistance to Coleosporium bletiae in Bletilla striata”
e2“the rust-resistant material of B. striata accessions exhibited faster and stronger defense responses to pathogen infection compared to susceptible plants”
e3“These responses were characterized by significant upregulation of DEGs associated with antioxidant defense systems, secondary metabolite biosynthesis, JA, SA, and BR signaling pathways, concurrent downregulation of DEGs involved in cell wall remodeling, and calcium-mediated signaling”
Claim · model-written summary
Rust-resistant Bletilla striata accessions showed downregulation of calcium-mediated signaling.
- Taxon
- Bletilla striataBletilla striatat0
- Material
- rust-resistant materiale2
- Outcome
- calcium-mediated signalinge3
- Direction
- downregulatione3
- Comparator
- susceptible plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptomics combined with physiological analysis provides insights into the mechanism of resistance to Coleosporium bletiae in Bletilla striata”
e2“the rust-resistant material of B. striata accessions exhibited faster and stronger defense responses to pathogen infection compared to susceptible plants”
e3“These responses were characterized by significant upregulation of DEGs associated with antioxidant defense systems, secondary metabolite biosynthesis, JA, SA, and BR signaling pathways, concurrent downregulation of DEGs involved in cell wall remodeling, and calcium-mediated signaling”
Claim · model-written summary
Rust pathogen inoculation enhanced the activity of defense-related enzymes CAT, PAL and β-1,3-glucanase in resistant Bletilla striata.
- Taxon
- Bletilla striataBletilla striatat0
- Treatment
- rust pathogen inoculatione4
- Interactor
- rust pathogene4pathogen
- Outcome
- activity of defense-related enzymese4
- Direction
- enhancede4
- Qualifier
- CAT, PAL, β-1,3-glucanasee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptomics combined with physiological analysis provides insights into the mechanism of resistance to Coleosporium bletiae in Bletilla striata”
e4“rust pathogen inoculation triggered rapid physiological responses in resistant plants, including enhanced activity of defense-related enzymes (CAT, PAL, β-1,3-glucanase, and chitinase)”
Claim · model-written summary
Rust pathogen inoculation enhanced chitinase activity in resistant Bletilla striata.
- Taxon
- Bletilla striataBletilla striatat0
- Treatment
- rust pathogen inoculatione4
- Interactor
- rust pathogene4pathogen
- Outcome
- activity of defense-related enzymese4
- Direction
- enhancede4
- Qualifier
- chitinasee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptomics combined with physiological analysis provides insights into the mechanism of resistance to Coleosporium bletiae in Bletilla striata”
e4“rust pathogen inoculation triggered rapid physiological responses in resistant plants, including enhanced activity of defense-related enzymes (CAT, PAL, β-1,3-glucanase, and chitinase)”
Claim · model-written summary
Rust pathogen inoculation led to early accumulation of osmolytes in resistant Bletilla striata.
- Taxon
- Bletilla striataBletilla striatat0
- Treatment
- rust pathogen inoculatione4
- Interactor
- rust pathogene4pathogen
- Outcome
- early accumulation of osmolytese5
- Direction
- increaseinferrede5
- Qualifier
- soluble sugars, soluble proteins, and prolinee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptomics combined with physiological analysis provides insights into the mechanism of resistance to Coleosporium bletiae in Bletilla striata”
e4“rust pathogen inoculation triggered rapid physiological responses in resistant plants, including enhanced activity of defense-related enzymes (CAT, PAL, β-1,3-glucanase, and chitinase)”
e5“early accumulation of osmolytes (soluble sugars, soluble proteins, and proline)”
Claim · model-written summary
The coordinated molecular and biochemical responses of Bletilla striata restricted pathogen colonization and spread.
- Taxon
- Bletilla striataBletilla striatat0
- Interactor
- pathogene6pathogen
- Outcome
- pathogen colonization and spreade6
- Direction
- restrictede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptomics combined with physiological analysis provides insights into the mechanism of resistance to Coleosporium bletiae in Bletilla striata”
e6“These coordinated molecular and biochemical responses effectively restricted pathogen colonization and spread”