Abstract
BACKGROUND: The multitrophic interactions in plant rhizosphere and endosphere can be beneficial or deleterious for the plant health. The parasitism by root-feeding nematodes is on the negative end of the interaction spectrum, and may be very difficult to control. Biological agents are a promising alternative to the environmentally harmful nematicides; however, their efficiency in natural soil often seems to be low due to their limited establishment and dispersal. Thus, understanding how the introduced biological agents interact with nematodes and the surrounding microbiota is necessary to improve sustainable management of root-feeding nematodes. Here, we conducted two experiments to study the effects of Pseudomonas protegens strain CHA0 (CHA0) on the performance of the root-knot nematode Meloidogyne incognita. In the first experiment, we compared M. incognita performance in natural and sterilized soil in the presence and absence of CHA0. In the second experiment, we studied the composition of microbes in the rhizosphere and endosphere of tomato plants grown in native soil in response to M. incognita and CHA0. RESULTS: We found that nematode performance, especially nematode reproduction, was significantly increased in native soil amended with CHA0. In addition, we found the highest relative abundance of Pseudomonas in tomato endosphere in response to nematode co-inoculations with CHA0, which suggests that root wounding, caused by nematodes, increased the entrance of inoculated and/or native Pseudomonas spp. As many Pseudomonas spp. are plant growth promoting, this may explain that plant growth was highest in this treatment. Furthermore, the rhizosphere of nematode-inoculated plants was enriched with Flavobacterium, Hydrogenophaga and Variovorax, which are genera generally associated with nematode-suppressive soils. On the other hand, other known nematode-suppressive genera such as Bacillus, Lysobacter, Devosia and Rhizobium were depleted in plants where nematodes were co-inoculated with CHA0, which may explain the higher nematode performance when plants were co-inoculated with CHA0. CONCLUSIONS: Our findings show that the effect of P. protegens strain CHA0 on M. incognita parasitism is influenced by the multitrophic interactions in the rhizosphere and endosphere of tomato plants. We must understand these interactions thoroughly to optimize sustainable means to mitigate the root-knot nematodes.
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Claims 7
Claim · model-written summary
CHA0 significantly increased Meloidogyne incognita reproduction in native soil (negative for the plant).
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- CHA0e1
- Interactor
- Meloidogyne incognita
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=60)
Meloidogyne incognitae2pest - Second interactor
- CHA0e1biocontrol_agent
- Outcome
- nematode reproductione1
- Direction
- increasede1
- Setting
- native soile1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=500)
Verbatim
t0“tomato-associated microbiota”
e1“We found that nematode performance, especially nematode reproduction, was significantly increased in native soil amended with CHA0”
e2“root-knot nematode Meloidogyne incognita”
Claim · model-written summary
The highest relative abundance of Pseudomonas was found in the tomato endosphere with nematode and CHA0 co-inoculation.
- Taxon
- tomatoSolanum lycopersicume3
- Organ / stage
- endospheree3
- Second interactor
- CHA0e3biocontrol_agent
- Outcome
- relative abundance of Pseudomonase3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“we found the highest relative abundance of Pseudomonas in tomato endosphere in response to nematode co-inoculations with CHA0”
Claim · model-written summary
Plant growth was highest in the nematode plus CHA0 co-inoculation treatment.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- nematode co-inoculations with CHA0e5
- Outcome
- plant growthe4
- Direction
- higheste4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato-associated microbiota”
e4“plant growth was highest in this treatment”
e5“in response to nematode co-inoculations with CHA0”
Claim · model-written summary
The rhizosphere of nematode-inoculated plants was enriched with Flavobacterium, Hydrogenophaga and Variovorax.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- rhizospheree6
- Outcome
- Flavobacterium, Hydrogenophaga and Variovoraxe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato-associated microbiota”
e6“the rhizosphere of nematode-inoculated plants was enriched with Flavobacterium, Hydrogenophaga and Variovorax”
Claim · model-written summary
Bacillus, Lysobacter and Devosia, nematode-suppressive genera, were depleted in plants co-inoculated with nematodes and CHA0.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- co-inoculated with CHA0e7
- Outcome
- Bacillus, Lysobacter, Devosiae7
- Direction
- depletede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato-associated microbiota”
e7“other known nematode-suppressive genera such as Bacillus, Lysobacter, Devosia and Rhizobium were depleted in plants where nematodes were co-inoculated with CHA0”
Claim · model-written summary
Rhizobium, a nematode-suppressive genus, was depleted in plants co-inoculated with nematodes and CHA0.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- co-inoculated with CHA0e7
- Outcome
- Rhizobiume7
- Direction
- depletede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato-associated microbiota”
e7“other known nematode-suppressive genera such as Bacillus, Lysobacter, Devosia and Rhizobium were depleted in plants where nematodes were co-inoculated with CHA0”
Claim · model-written summary
The effect of CHA0 on M. incognita parasitism is influenced by multitrophic interactions in the rhizosphere and endosphere of tomato.
- Taxon
- tomatoSolanum lycopersicume7
- Outcome
- M. incognita parasitisme7
- Qualifier
- multitrophic interactions in the rhizosphere and endospheree7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“the effect of P. protegens strain CHA0 on M. incognita parasitism is influenced by the multitrophic interactions in the rhizosphere and endosphere of tomato plants”
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See all 441 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.