Abstract
To identify the key genes conferring resistance to soybean cyst nematode race 4 (SCN4, Heterodera glycines), this study utilized 280 recombinant inbred lines (RILs) derived from the resistant cultivar Huipizhiheidou (HPD) and the susceptible cultivar Jindou23 (JD23). Through phenotypic characterization and a genome-wide association study (GWAS), a genomic region (Gm18:1,223,546–1,782,241) on chromosome 18 was mapped, yielding 14 candidate genes. GmPDH1 was validated as a critical resistance gene using reverse transcription quantitative PCR (RT-qPCR) and Kompetitive Allele Specific PCR (KASP) marker M0526. RT-qPCR revealed that GmPDH1 expression in HPD roots was upregulated 9 days post-inoculation with SCN4 compared to uninoculated controls. KASP genotyping showed that marker M0526 efficiently distinguished between resistant and susceptible plants in natural populations: 71.05% of the resistant accessions exhibited resistant or moderately resistant genotypes, whereas 81.03% of the susceptible accessions showed susceptible or highly susceptible genotypes. Functional validation demonstrated that overexpression of GmPDH1 significantly enhanced SCN4 resistance in the susceptible cultivars JD23 and Jack, whereas CRISPR/Cas9-mediated knockout of GmPDH1 in HPD attenuated its resistance. This study confirmed GmPDH1 as a key gene governing SCN4 resistance and developed an efficient molecular marker, M0526, providing theoretical insights and technical tools for dissecting nematode resistance mechanisms and advancing soybean disease-resistant breeding.
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Claims 7
Claim · model-written summary
A chromosome 18 region associated with resistance yielded 14 candidate genes.
- Taxon
- Soybean
Glycine maxt0 - Interactor
- Cyst Nematode Race 4t0pathogen
- Outcome
- candidate genese0
- Value
- 14e0
- Qualifier
- Gm18:1,223,546–1,782,241e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a genomic region (Gm18:1,223,546–1,782,241) on chromosome 18 was mapped, yielding 14 candidate genes”
t0“Soybean Against Cyst Nematode Race 4”
Claim · model-written summary
GmPDH1 was validated as a resistance gene.
- Taxon
- SoybeanGlycine maxt0
- Interactor
- Cyst Nematode Race 4t0pathogen
- Gene
- GmPDH1e0
- Outcome
- critical resistance genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmPDH1 was validated as a critical resistance gene using reverse transcription quantitative PCR (RT-qPCR) and Kompetitive Allele Specific PCR (KASP) marker M0526”
t0“Soybean Against Cyst Nematode Race 4”
Claim · model-written summary
GmPDH1 expression increased in HPD roots nine days after SCN4 inoculation.
- Taxon
- soybeanGlycine maxt0
- Material
- HPDe0
- Organ / stage
- rootse0
- Treatment
- inoculation with SCN4e0
- Interactor
- SCN4e0pathogen
- Gene
- GmPDH1e0
- Outcome
- GmPDH1 expressione0
- Direction
- upregulatede0
- Qualifier
- 9 days post-inoculatione0
- Comparator
- uninoculated controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmPDH1 expression in HPD roots was upregulated 9 days post-inoculation with SCN4 compared to uninoculated controls”
t0“in Soybean”
Claim · model-written summary
Marker M0526 distinguished resistant and susceptible plants in natural populations.
- Taxon
- SoybeanGlycine maxt0
- Interactor
- Cyst Nematode Race 4t0pathogen
- Gene
- M0526e0
- Outcome
- distinguished between resistant and susceptible plantse0
- Value
- 71.05%e0
- Qualifier
- 81.03%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“marker M0526 efficiently distinguished between resistant and susceptible plants in natural populations: 71.05% of the resistant accessions exhibited resistant or moderately resistant genotypes, whereas 81.03% of the susceptible accessions showed susceptible or highly susceptible genotypes”
t0“Soybean Against Cyst Nematode Race 4”
Claim · model-written summary
Overexpressing GmPDH1 significantly increased SCN4 resistance in JD23.
- Taxon
- soybeanGlycine maxt0
- Material
- JD23e0
- Treatment
- overexpression of GmPDH1e0
- Interactor
- SCN4e0pathogen
- Gene
- GmPDH1e0
- Outcome
- SCN4 resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of GmPDH1 significantly enhanced SCN4 resistance in the susceptible cultivars JD23 and Jack”
t0“in Soybean”
Claim · model-written summary
Overexpressing GmPDH1 significantly increased SCN4 resistance in Jack.
- Taxon
- soybeanGlycine maxt0
- Material
- Jacke0
- Treatment
- overexpression of GmPDH1e0
- Interactor
- SCN4e0pathogen
- Gene
- GmPDH1e0
- Outcome
- SCN4 resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of GmPDH1 significantly enhanced SCN4 resistance in the susceptible cultivars JD23 and Jack”
t0“in Soybean”
Claim · model-written summary
Knocking out GmPDH1 attenuated resistance in HPD.
- Taxon
- SoybeanGlycine maxt0
- Material
- HPDe0
- Treatment
- CRISPR/Cas9-mediated knockout of GmPDH1e0
- Interactor
- Cyst Nematode Race 4t0pathogen
- Gene
- GmPDH1e0
- Outcome
- resistancee0
- Direction
- attenuatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRISPR/Cas9-mediated knockout of GmPDH1 in HPD attenuated its resistance”
t0“Soybean Against Cyst Nematode Race 4”
The same questions in other papers
loss of function · disease · Glycine max (soybean)Positive and negative results
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- positive
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