Paper · 2025 · Brazilian Journal of Biology

Combined use of humic acids and Trichoderma harzianum as sustainable alternatives to alliviate salt stress in bell pepper

R. H. Tirado-Malaver; R. Tirado-Lara

14
claims
30
verbatim quotes
2
citations
3
research areas
✓
Open access · cc-by

Abstract

Bell pepper is a vegetable with beneficial properties for human nutrition. However, salinity is an abiotic factor affecting bell pepper yield in arid and semi-arid areas of Peru. The objective was to determine the combined effect of humic acid (HA) with Trichoderma harzianum (TH) as sustainable alternatives to increase the yield of bell peppers subjected to salt stress. The experiment was carried in field out during the 2023 and 2024 seasons design with a 2 x 3 x 2 factorial scheme, referring to the electrical conductivity (EC) of the irrigation water (0 and 4 dSm-1), HA (0, 15 and 30 L ha-1) and TH (0 and 1 kg ha-1). Agronomic and yield-related parameters were analyzed. The results showed that the saline soil accompanied by irrigations of 0 and 4 dS m-1 of EC, showed a significant decrease in agronomic parameters and an increase in concentration of sodium and chlorine in bell pepper leaves. However, the application of 30 L ha-1 of HA with TH increase in plant height (86.53 y 86,42%), higher root dry weight (95.44 y 95.32%) and plant dry weight (90.46 y 90.41%), also, greatly improved fruit length 64.6 y 63.74%) and width (58.47 y 57.31%), significantly increased fruit content per plant (91.49 y 91.30%), fruit weight per plant (93.29 and 93.15%) and total yield (89.54 and 89.23%) in relation to plants subjected to salt stress. It also significantly increases the concentration of potassium and calcium in the leaves (37.88 and 48.71%), K+/ Na+ ratio (72.07 and 74.93%) and proline content (58.60 and 59.31%) superior to the control, as a defense mechanism against salt stress. Therefore, as the dose of humic acids combined with Trichoderma harzianum is increased, the plant shows tolerance to salinity, being a sustainable alternative of bell pepper to reduce salt stress in arid and semi-arid areas of Peru.

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Claims 14

Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Salinity significantly decreased bell pepper agronomic parameters.

Taxon
bell peppere0
Treatment
saline soil accompanied by irrigations of 0 and 4 dS m-1 of ECe0
Outcome
agronomic parameterse0
Direction
decreasee0

Not reportedComparator

Verbatim

e0

“The results showed that the saline soil accompanied by irrigations of 0 and 4 dS m-1 of EC, showed a significant decrease in agronomic parameters and an increase in concentration of sodium and chlorine in bell pepper leaves.”

✓ located verbatim in the source · abstract, characters 680–904 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Salinity increased sodium and chlorine concentrations in bell pepper leaves.

Taxon
bell peppere0
Organ / stage
leavese0
Treatment
saline soil accompanied by irrigations of 0 and 4 dS m-1 of ECe0
Outcome
concentration of sodium and chlorinee0
Direction
increasee0

Not reportedComparator

Verbatim

e0

“The results showed that the saline soil accompanied by irrigations of 0 and 4 dS m-1 of EC, showed a significant decrease in agronomic parameters and an increase in concentration of sodium and chlorine in bell pepper leaves.”

✓ located verbatim in the source · abstract, characters 680–904 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

HA with TH improved bell pepper plant height relative to salt-stressed plants (86.53 y 86,42%).

Taxon
bell peppert0
Treatment
30 L ha-1 of HA with THe0
Interactor
THe0symbiont
Outcome
plant heighte0
Direction
increasee0
Value
86.53 y 86,42%e0
Comparator
plants subjected to salt stresse0

Verbatim

e0

“However, the application of 30 L ha-1 of HA with TH increase in plant height (86.53 y 86,42%), higher root dry weight (95.44 y 95.32%) and plant dry weight (90.46 y 90.41%), also, greatly improved fruit length 64.6 y 63.74%) and width (58.47 y 57.31%), significantly increased fruit content per plant (91.49 y 91.30%), fruit weight per plant (93.29 and 93.15%) and total yield (89.54 and 89.23%) in relation to plants subjected to salt stress.”

✓ located verbatim in the source · abstract, characters 905–1348 · show in text ↑
t0

“bell pepper”

✓ located verbatim in the source · title, characters 110–121 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

HA with TH improved bell pepper root dry weight relative to salt-stressed plants (95.44 y 95.32%).

Taxon
bell peppert0
Treatment
30 L ha-1 of HA with THe0
Interactor
THe0symbiont
Outcome
root dry weighte0
Direction
highere0
Value
95.44 y 95.32%e0
Comparator
plants subjected to salt stresse0

Verbatim

e0

“However, the application of 30 L ha-1 of HA with TH increase in plant height (86.53 y 86,42%), higher root dry weight (95.44 y 95.32%) and plant dry weight (90.46 y 90.41%), also, greatly improved fruit length 64.6 y 63.74%) and width (58.47 y 57.31%), significantly increased fruit content per plant (91.49 y 91.30%), fruit weight per plant (93.29 and 93.15%) and total yield (89.54 and 89.23%) in relation to plants subjected to salt stress.”

✓ located verbatim in the source · abstract, characters 905–1348 · show in text ↑
t0

“bell pepper”

✓ located verbatim in the source · title, characters 110–121 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

HA with TH improved bell pepper plant dry weight relative to salt-stressed plants (90.46 y 90.41%).

Taxon
bell peppert0
Treatment
30 L ha-1 of HA with THe0
Interactor
THe0symbiont
Outcome
plant dry weighte0
Direction
highere0
Value
90.46 y 90.41%e0
Comparator
plants subjected to salt stresse0

Verbatim

e0

“However, the application of 30 L ha-1 of HA with TH increase in plant height (86.53 y 86,42%), higher root dry weight (95.44 y 95.32%) and plant dry weight (90.46 y 90.41%), also, greatly improved fruit length 64.6 y 63.74%) and width (58.47 y 57.31%), significantly increased fruit content per plant (91.49 y 91.30%), fruit weight per plant (93.29 and 93.15%) and total yield (89.54 and 89.23%) in relation to plants subjected to salt stress.”

✓ located verbatim in the source · abstract, characters 905–1348 · show in text ↑
t0

“bell pepper”

✓ located verbatim in the source · title, characters 110–121 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

HA with TH improved bell pepper fruit length relative to salt-stressed plants (64.6 y 63.74%).

Taxon
bell peppert0
Treatment
30 L ha-1 of HA with THe0
Interactor
THe0symbiont
Outcome
fruit lengthe0
Direction
improvede0
Value
64.6 y 63.74%e0
Comparator
plants subjected to salt stresse0

Verbatim

e0

“However, the application of 30 L ha-1 of HA with TH increase in plant height (86.53 y 86,42%), higher root dry weight (95.44 y 95.32%) and plant dry weight (90.46 y 90.41%), also, greatly improved fruit length 64.6 y 63.74%) and width (58.47 y 57.31%), significantly increased fruit content per plant (91.49 y 91.30%), fruit weight per plant (93.29 and 93.15%) and total yield (89.54 and 89.23%) in relation to plants subjected to salt stress.”

✓ located verbatim in the source · abstract, characters 905–1348 · show in text ↑
t0

“bell pepper”

✓ located verbatim in the source · title, characters 110–121 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

HA with TH improved bell pepper width relative to salt-stressed plants (58.47 y 57.31%).

Taxon
bell peppert0
Treatment
30 L ha-1 of HA with THe0
Interactor
THe0symbiont
Outcome
widthe0
Direction
improvede0
Value
58.47 y 57.31%e0
Comparator
plants subjected to salt stresse0

Verbatim

e0

“However, the application of 30 L ha-1 of HA with TH increase in plant height (86.53 y 86,42%), higher root dry weight (95.44 y 95.32%) and plant dry weight (90.46 y 90.41%), also, greatly improved fruit length 64.6 y 63.74%) and width (58.47 y 57.31%), significantly increased fruit content per plant (91.49 y 91.30%), fruit weight per plant (93.29 and 93.15%) and total yield (89.54 and 89.23%) in relation to plants subjected to salt stress.”

✓ located verbatim in the source · abstract, characters 905–1348 · show in text ↑
t0

“bell pepper”

✓ located verbatim in the source · title, characters 110–121 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

HA with TH improved bell pepper fruit content per plant relative to salt-stressed plants (91.49 y 91.30%).

Taxon
bell peppert0
Treatment
30 L ha-1 of HA with THe0
Interactor
THe0symbiont
Outcome
fruit content per plante0
Direction
increasede0
Value
91.49 y 91.30%e0
Comparator
plants subjected to salt stresse0

Verbatim

e0

“However, the application of 30 L ha-1 of HA with TH increase in plant height (86.53 y 86,42%), higher root dry weight (95.44 y 95.32%) and plant dry weight (90.46 y 90.41%), also, greatly improved fruit length 64.6 y 63.74%) and width (58.47 y 57.31%), significantly increased fruit content per plant (91.49 y 91.30%), fruit weight per plant (93.29 and 93.15%) and total yield (89.54 and 89.23%) in relation to plants subjected to salt stress.”

✓ located verbatim in the source · abstract, characters 905–1348 · show in text ↑
t0

“bell pepper”

✓ located verbatim in the source · title, characters 110–121 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

HA with TH improved bell pepper fruit weight per plant relative to salt-stressed plants (93.29 and 93.15%).

Taxon
bell peppert0
Treatment
30 L ha-1 of HA with THe0
Interactor
THe0symbiont
Outcome
fruit weight per plante0
Direction
increasede0
Value
93.29 and 93.15%e0
Comparator
plants subjected to salt stresse0

Verbatim

e0

“However, the application of 30 L ha-1 of HA with TH increase in plant height (86.53 y 86,42%), higher root dry weight (95.44 y 95.32%) and plant dry weight (90.46 y 90.41%), also, greatly improved fruit length 64.6 y 63.74%) and width (58.47 y 57.31%), significantly increased fruit content per plant (91.49 y 91.30%), fruit weight per plant (93.29 and 93.15%) and total yield (89.54 and 89.23%) in relation to plants subjected to salt stress.”

✓ located verbatim in the source · abstract, characters 905–1348 · show in text ↑
t0

“bell pepper”

✓ located verbatim in the source · title, characters 110–121 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

HA with TH improved bell pepper total yield relative to salt-stressed plants (89.54 and 89.23%).

Taxon
bell peppert0
Treatment
30 L ha-1 of HA with THe0
Interactor
THe0symbiont
Outcome
total yielde0
Direction
increasede0
Value
89.54 and 89.23%e0
Comparator
plants subjected to salt stresse0

Verbatim

e0

“However, the application of 30 L ha-1 of HA with TH increase in plant height (86.53 y 86,42%), higher root dry weight (95.44 y 95.32%) and plant dry weight (90.46 y 90.41%), also, greatly improved fruit length 64.6 y 63.74%) and width (58.47 y 57.31%), significantly increased fruit content per plant (91.49 y 91.30%), fruit weight per plant (93.29 and 93.15%) and total yield (89.54 and 89.23%) in relation to plants subjected to salt stress.”

✓ located verbatim in the source · abstract, characters 905–1348 · show in text ↑
t0

“bell pepper”

✓ located verbatim in the source · title, characters 110–121 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The treatment increased bell pepper concentration of potassium relative to control.

Taxon
bell peppert0
Treatment
30 L ha-1 of HA with THe1
Interactor
THe1symbiont
Outcome
concentration of potassiume0
Direction
increasese0
Value
37.88 and 48.71%e0
Comparator
controle0

Verbatim

e0

“It also significantly increases the concentration of potassium and calcium in the leaves (37.88 and 48.71%), K+/ Na+ ratio (72.07 and 74.93%) and proline content (58.60 and 59.31%) superior to the control, as a defense mechanism against salt stress.”

✓ located verbatim in the source · abstract, characters 1349–1598 · show in text ↑
e1

“the application of 30 L ha-1 of HA with TH”

✓ located verbatim in the source · abstract, characters 914–956 · show in text ↑
t0

“bell pepper”

✓ located verbatim in the source · title, characters 110–121 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The treatment increased bell pepper calcium relative to control.

Taxon
bell peppert0
Treatment
30 L ha-1 of HA with THe1
Interactor
THe1symbiont
Outcome
calciume0
Direction
increasese0
Value
37.88 and 48.71%e0
Comparator
controle0

Verbatim

e0

“It also significantly increases the concentration of potassium and calcium in the leaves (37.88 and 48.71%), K+/ Na+ ratio (72.07 and 74.93%) and proline content (58.60 and 59.31%) superior to the control, as a defense mechanism against salt stress.”

✓ located verbatim in the source · abstract, characters 1349–1598 · show in text ↑
e1

“the application of 30 L ha-1 of HA with TH”

✓ located verbatim in the source · abstract, characters 914–956 · show in text ↑
t0

“bell pepper”

✓ located verbatim in the source · title, characters 110–121 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The treatment increased bell pepper K+/ Na+ ratio relative to control.

Taxon
bell peppert0
Treatment
30 L ha-1 of HA with THe1
Interactor
THe1symbiont
Outcome
K+/ Na+ ratioe0
Direction
increasese0
Value
72.07 and 74.93%e0
Comparator
controle0

Verbatim

e0

“It also significantly increases the concentration of potassium and calcium in the leaves (37.88 and 48.71%), K+/ Na+ ratio (72.07 and 74.93%) and proline content (58.60 and 59.31%) superior to the control, as a defense mechanism against salt stress.”

✓ located verbatim in the source · abstract, characters 1349–1598 · show in text ↑
e1

“the application of 30 L ha-1 of HA with TH”

✓ located verbatim in the source · abstract, characters 914–956 · show in text ↑
t0

“bell pepper”

✓ located verbatim in the source · title, characters 110–121 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The treatment increased bell pepper proline content relative to control.

Taxon
bell peppert0
Treatment
30 L ha-1 of HA with THe1
Interactor
THe1symbiont
Outcome
proline contente0
Direction
increasese0
Value
58.60 and 59.31%e0
Comparator
controle0

Verbatim

e0

“It also significantly increases the concentration of potassium and calcium in the leaves (37.88 and 48.71%), K+/ Na+ ratio (72.07 and 74.93%) and proline content (58.60 and 59.31%) superior to the control, as a defense mechanism against salt stress.”

✓ located verbatim in the source · abstract, characters 1349–1598 · show in text ↑
e1

“the application of 30 L ha-1 of HA with TH”

✓ located verbatim in the source · abstract, characters 914–956 · show in text ↑
t0

“bell pepper”

✓ located verbatim in the source · title, characters 110–121 · show in text ↑

Trichoderma harzianum · molecular: metabolites · Capsicum annuum (sweet pepper)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. no effect
See all 2 papers →

Trichoderma harzianum · yield · Capsicum annuum (sweet pepper)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

salinity · molecular: metabolites · Capsicum annuum (sweet pepper)Positive and negative results

7 papers: 1 positive, 6 negative or no effect

5 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. negative

3 papers more in this group

See all 7 papers →

other · nutrient: content · Capsicum annuum (sweet pepper)Positive and negative results

11 papers: 9 positive, 2 negative or no effect

8 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. no effect
  3. positive

7 papers more in this group

See all 11 papers →

other · growth · Capsicum annuum (sweet pepper)Positive and negative results

22 papers: 19 positive, 3 negative or no effect

18 papers in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. no effect
  3. no effect

18 papers more in this group

See all 22 papers →

other · yield · Capsicum annuum (sweet pepper)Positive and negative results

28 papers: 25 positive, 3 negative or no effect

24 papers in the same direction as this paper · 2 in the opposite direction · 1 reporting no difference from an alternative

  1. no effect
  2. no effect
  3. no effect

24 papers more in this group

See all 28 papers →

irrigation · molecular: metabolites · Capsicum annuum (sweet pepper)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. negative
See all 2 papers →

other · physiology: osmolytes · Capsicum annuum (sweet pepper)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

8 of 9 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.