Abstract
Background: Broomcorn millet (Panicum miliaceum L.), a drought-tolerant C4 crop, is crucial for agricultural resilience in arid regions. Lipoxygenases (LOXs), key enzymes in plant stress responses, have not been studied in broomcorn millet. This study aimed to identify LOX genes in broomcorn millet and elucidate their role in drought tolerance. Methods: We employed bioinformatics and physiological analyses to identify LOX genes in broomcorn millet. Expression profiles were assessed in different organs, and drought stress responses were evaluated in tolerant (HSZ, YXDHM) and sensitive (YS10) varieties. Antioxidant enzyme activities (SOD, POD, CAT) and malondialdehyde (MDA) levels were measured. Results: Twelve LOX genes were identified, classified into three subfamilies, and mapped across seven chromosomes. These genes contained stress-responsive cis-elements and showed organ-specific expression, with PmLOX5 exhibiting no detectable expression. Under drought stress, tolerant varieties showed elevated antioxidant activities and reduced MDA accumulation. PmLOX2, a homolog of Arabidopsis AtLOX1/AtLOX5, was significantly induced in tolerant varieties, correlating with enhanced antioxidant capacity and reduced oxidative damage. Conclusions:PmLOX genes, particularly PmLOX2, play a pivotal role in drought tolerance by modulating ROS scavenging and membrane protection. This study provides a foundation for leveraging LOX genes to improve drought resilience in broomcorn millet and related crops.
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Claims 8
Claim · model-written summary
Twelve LOX genes were identified in broomcorn millet, falling into three subfamilies across seven chromosomes.
- Taxon
- Broomcorn Millet
Panicum miliaceumt0 - Gene
- LOX genese0
- Outcome
- identified, classified into three subfamilies, and mapped across seven chromosomese0
- Value
- Twelvee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Twelve LOX genes were identified, classified into three subfamilies, and mapped across seven chromosomes.”
t0“Broomcorn Millet (Panicum miliaceum L.)”
Claim · model-written summary
PmLOX5 had no detectable expression.
- Taxon
- Broomcorn MilletPanicum miliaceumt0
- Gene
- PmLOX5e0
- Outcome
- no detectable expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PmLOX5 exhibiting no detectable expression.”
t0“Broomcorn Millet”
Claim · model-written summary
Drought-tolerant broomcorn millet varieties had elevated antioxidant enzyme activities under drought.
- Taxon
- Broomcorn MilletPanicum miliaceumt0
- Material
- tolerant varietiese0
- Treatment
- drought stresse0
- Outcome
- antioxidant activitiese0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought stress, tolerant varieties showed elevated antioxidant activities and reduced MDA accumulation.”
t0“Broomcorn Millet”
Claim · model-written summary
Drought-tolerant broomcorn millet varieties had reduced MDA accumulation under drought.
- Taxon
- Broomcorn MilletPanicum miliaceumt0
- Material
- tolerant varietiese0
- Treatment
- drought stresse0
- Outcome
- MDA accumulatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought stress, tolerant varieties showed elevated antioxidant activities and reduced MDA accumulation.”
t0“Broomcorn Millet”
Claim · model-written summary
PmLOX2 was significantly induced in drought-tolerant broomcorn millet varieties.
- Taxon
- Broomcorn MilletPanicum miliaceumt0
- Material
- tolerant varietiese0
- Treatment
- drought stresse1
- Gene
- PmLOX2e0
- Outcome
- PmLOX2e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PmLOX2, a homolog of Arabidopsis AtLOX1/AtLOX5, was significantly induced in tolerant varieties”
e1“Under drought stress, tolerant varieties showed elevated antioxidant activities and reduced MDA accumulation.”
t0“Broomcorn Millet”
Claim · model-written summary
PmLOX2 induction correlated with enhanced antioxidant capacity.
- Taxon
- Broomcorn MilletPanicum miliaceumt0
- Gene
- PmLOX2e0
- Outcome
- antioxidant capacitye0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PmLOX2, a homolog of Arabidopsis AtLOX1/AtLOX5, was significantly induced in tolerant varieties, correlating with enhanced antioxidant capacity and reduced oxidative damage.”
t0“Broomcorn Millet”
Claim · model-written summary
PmLOX2 induction correlated with reduced oxidative damage.
- Taxon
- Broomcorn MilletPanicum miliaceumt0
- Gene
- PmLOX2e0
- Outcome
- oxidative damagee0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PmLOX2, a homolog of Arabidopsis AtLOX1/AtLOX5, was significantly induced in tolerant varieties, correlating with enhanced antioxidant capacity and reduced oxidative damage.”
t0“Broomcorn Millet”
Claim · model-written summary
The authors infer that PmLOX genes, especially PmLOX2, promote drought tolerance through ROS scavenging and membrane protection.
- Taxon
- Broomcorn MilletPanicum miliaceumt0
- Gene
- PmLOX2e0
- Outcome
- drought tolerancee0
- Qualifier
- modulating ROS scavenging and membrane protectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PmLOX genes, particularly PmLOX2, play a pivotal role in drought tolerance by modulating ROS scavenging and membrane protection.”
t0“Broomcorn Millet”
The same questions in other papers
drought · physiology: oxidative · Panicum miliaceum (proso millet)Positive and negative results
1 in the opposite direction
- negative
drought · molecular: metabolites · Panicum miliaceum (proso millet)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.