Abstract
Abstract Continuous cropping of bananas leads to Fusarium wilt, affecting crop health, yet employing biocontrol bacteria to modulate rhizosphere microbial communities may offer effective disease suppression. This study revealed the secondary succession characteristics of rhizosphere microbiota in banana fields after more than 5‐year continuous cropping and assessed the suppressive effects induced by biocontrol strain Bacillus subtilis R31. Through high‐throughput sequencing, we observed a convergent enrichment of core bacterial genera Burkholderia ‐ Dyella and Arthrobacter ‐ Ralstonia in naturally suppressive and R31‐treated suppressive soils, indicating broad‐spectrum disease‐suppressive soil traits induced by R31. R31 significantly enhanced associations between Streptomyces and the rhizosphere core community while weakening Burkholderia 's linkage with membrane transport and energy metabolism pathways; moreover, it strengthened positive correlations between Rhizobium and terpenoid and polyketide metabolism ( r = 0.65, p < 0.01). Culture‐dependent assays showed that among 46 isolates from root and rhizosphere, those with high activities of indole‐3‐acetic acid, protease, cellulase, chitinase, and β ‐1,3‐glucanase exhibited pronounced antagonistic activities against Fusarium oxysporum . Although R31 was undetectable in the rhizosphere in the second year, its modulation of rhizosphere function persisted, displaying a “legacy effect” consistent with the priority effects theory, whereby early colonizing beneficial communities resist subsequent pathogen invasions. R31 induced functional bacteria colonization in nascent banana root hair tissues, establishing new microbiome patterns that contributed to long‐term disease suppression. Pot experiments further indicated that endophytic bacteria within roots exhibited stronger Fusarium wilt control than rhizosphere isolates. However, single‐strain treatments frequently led to sheath rot co‐infection, suggesting that synergistic actions of multiple strains might be more effective for disease suppression. This study highlighted R31's potential to sustainably modulate the rhizosphere microbiome, enhance enzyme activity, and promote beneficial bacteria colonization, laying the groundwork for constructing efficient synthetic bacterial communities for biocontrol.
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Claims 12
Claim · model-written summary
Suppressive and R31-treated banana soils showed convergent enrichment of two core bacterial groups.
Verbatim
e0“we observed a convergent enrichment of core bacterial genera Burkholderia ‐ Dyella and Arthrobacter ‐ Ralstonia in naturally suppressive and R31‐treated suppressive soils”
t0“banana”
Claim · model-written summary
R31 significantly enhanced associations between Streptomyces and the banana rhizosphere core community.
- Taxon
- bananat0
- Treatment
- R31e0
- Interactor
- Streptomyces
Streptomycese0symbiont - Outcome
- associations between Streptomyces and the rhizosphere core communitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“R31 significantly enhanced associations between Streptomyces and the rhizosphere core community”
t0“banana”
Claim · model-written summary
R31 weakened the linkage between Burkholderia and membrane transport and energy metabolism pathways.
- Taxon
- bananat0
- Treatment
- R31e1
- Interactor
- Burkholderia
Burkholderiae0symbiont - Outcome
- Burkholderia 's linkage with membrane transport and energy metabolism pathwayse0
- Direction
- weakeninge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“weakening Burkholderia 's linkage with membrane transport and energy metabolism pathways”
t0“banana”
e1“R31 significantly enhanced associations”
Claim · model-written summary
R31 strengthened positive correlations between Rhizobium and terpenoid and polyketide metabolism.
- Taxon
- bananat0
- Treatment
- R31e1
- Outcome
- correlations between Rhizobium and terpenoid and polyketide metabolisme0
- Direction
- strengthenede0
- Value
- r = 0.65e0
RhizobiumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it strengthened positive correlations between Rhizobium and terpenoid and polyketide metabolism ( r = 0.65, p < 0.01)”
e1“R31 significantly enhanced associations”
t0“banana”
Claim · model-written summary
Root and rhizosphere isolates with high enzyme and indole-3-acetic acid activities strongly antagonized Fusarium oxysporum.
- Taxon
- bananat0
- Interactor
- Fusarium oxysporum
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=60)
Fusarium oxysporume0pathogen - Outcome
- pronounced antagonistic activities against Fusarium oxysporume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=500)
Verbatim
e0“among 46 isolates from root and rhizosphere, those with high activities of indole‐3‐acetic acid, protease, cellulase, chitinase, and β ‐1,3‐glucanase exhibited pronounced antagonistic activities against Fusarium oxysporum”
t0“banana”
Claim · model-written summary
R31 was undetectable in the banana rhizosphere in the second year.
Verbatim
e0“R31 was undetectable in the rhizosphere in the second year”
t0“banana”
Claim · model-written summary
R31 modulation of banana rhizosphere function persisted into the second year.
- Taxon
- bananat0
- Treatment
- R31e1
- Outcome
- modulation of rhizosphere function persistede0
- Qualifier
- in the second yeare1
Not reportedComparatorDirection
Verbatim
e0“its modulation of rhizosphere function persisted, displaying a “legacy effect””
e1“R31 was undetectable in the rhizosphere in the second year”
t0“banana”
Claim · model-written summary
R31 induced functional bacteria colonization in nascent banana root hair tissues.
- Taxon
- bananae0
- Organ / stage
- nascent banana root hair tissuese0
- Treatment
- R31e0
- Outcome
- functional bacteria colonization in nascent banana root hair tissuese0
Not reportedComparatorDirection
Verbatim
e0“R31 induced functional bacteria colonization in nascent banana root hair tissues”
Claim · model-written summary
The R31-induced microbiome patterns contributed to long-term banana disease suppression.
Verbatim
e0“establishing new microbiome patterns that contributed to long‐term disease suppression”
e1“R31 induced functional bacteria colonization in nascent banana root hair tissues”
Claim · model-written summary
Root endophytic bacteria controlled Fusarium wilt more strongly than rhizosphere isolates in pot experiments.
- Taxon
- bananat0
- Treatment
- endophytic bacteria within rootse0
- Outcome
- Fusarium wilt controle0
- Direction
- strongere0
- Comparator
- rhizosphere isolatese0
FusariumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“endophytic bacteria within roots exhibited stronger Fusarium wilt control than rhizosphere isolates”
t0“banana”
Claim · model-written summary
Single-strain treatments frequently led to banana sheath rot co-infection.
Not reportedComparatorDirection
Verbatim
e0“single‐strain treatments frequently led to sheath rot co‐infection”
t0“banana”
Claim · model-written summary
The authors suggest multiple strains may better suppress banana disease.
Verbatim
e0“synergistic actions of multiple strains might be more effective for disease suppression”
t0“banana”
The same questions in other papers
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See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.