Paper · 2024 · The future of Horticulture

Optimizing In Vitro Micrografting Technique for Kalamata Olive (Olea europaea L.) and assessment their genetic fidelity

7
claims
14
verbatim quotes
0
citations
3
research areas
✓
Open access

Abstract

This study evaluates the extent of micro grafting success of the Kalamata cultivar (Olea europaea L.) onto two rootstocks, Kronaki and Coratina, as well as examining the influence of various factors on micrografting outcomes. Additionally, the research investigates the impact of mineral composition in the nutrient medium on the in vitro development of these rootstocks. The findings demonstrate that, cultivars and growth medium significantly impact germination performance, the Kronaki cultivar exhibiting a higher germination rate (74%) compared to Coratina (62.5%). Moreover, the Murashige and Skoog (MS) medium outperformed the Olive Medium (OM) in terms of survival rates and shoot elongation. Specifically, MS medium facilitated greater shoot growth (4.85 cm on average) compared to OM medium (4.2 cm). Kronaki showed higher shoot lengths (5.20 cm) than Coratina cultivar (4.5 cm) on MS that superiority than OM medium. During acclimatization, Kronaki also exhibited higher survival rates (72.5%) compared to Coratina (59%). Genetic fidelity analysis using Inter-Simple Sequence Repeat (ISSR) markers indicated a high level of genetic stability, with 96% monomorphic bands and only 4% polymorphism observed. These results highlight the critical role of genotype and growth medium in optimizing micrografting success and maintaining genetic integrity in olive rootstock propagation.

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Claims 7

Observationobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Kronaki germination exceeded Coratina, 74% versus 62.5%.

Taxon
OliveOlea europaeae1
Material
Kronakie0
Outcome
germination ratee0
Direction
highere0
Value
74%e0
Qualifier
62.5%e0
Comparator
Coratinae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Olea europaea
Olea europaea · TaxonHans Hillewaert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“cultivars and growth medium significantly impact germination performance, the Kronaki cultivar exhibiting a higher germination rate (74%) compared to Coratina (62.5%).”

✓ located verbatim in the source · abstract, characters 403–570 · show in text ↑
e1

“Olive (Olea europaea L.)”

✓ located verbatim in the source · title, characters 57–81 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

MS medium produced higher survival rates than OM medium.

Taxon
OliveOlea europaeae1
Treatment
Murashige and Skoog (MS) mediume0
Outcome
survival ratese0
Direction
outperformede0
Comparator
Olive Medium (OM)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Olea europaea
Olea europaea · TaxonHans Hillewaert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the Murashige and Skoog (MS) medium outperformed the Olive Medium (OM) in terms of survival rates and shoot elongation.”

✓ located verbatim in the source · abstract, characters 581–700 · show in text ↑
e1

“Olive (Olea europaea L.)”

✓ located verbatim in the source · title, characters 57–81 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

MS medium produced greater shoot growth than OM, averaging 4.85 versus 4.2 cm.

Taxon
OliveOlea europaeae1
Treatment
MS mediume0
Outcome
shoot growthe0
Direction
greatere0
Value
4.85 cme0
Qualifier
4.2 cme0
Comparator
OM mediume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Olea europaea
Olea europaea · TaxonHans Hillewaert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“MS medium facilitated greater shoot growth (4.85 cm on average) compared to OM medium (4.2 cm).”

✓ located verbatim in the source · abstract, characters 715–810 · show in text ↑
e1

“Olive (Olea europaea L.)”

✓ located verbatim in the source · title, characters 57–81 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Kronaki shoots on MS were longer than Coratina shoots, 5.20 versus 4.5 cm.

Taxon
OliveOlea europaeae1
Material
Kronakie0
Outcome
shoot lengthse0
Direction
highere0
Value
5.20 cme0
Qualifier
4.5 cme0
Comparator
Coratina cultivare0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Olea europaea
Olea europaea · TaxonHans Hillewaert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Kronaki showed higher shoot lengths (5.20 cm) than Coratina cultivar (4.5 cm) on MS”

✓ located verbatim in the source · abstract, characters 811–894 · show in text ↑
e1

“Olive (Olea europaea L.)”

✓ located verbatim in the source · title, characters 57–81 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Kronaki acclimatization survival exceeded Coratina, 72.5% versus 59%.

Taxon
OliveOlea europaeae1
Material
Kronakie0
Outcome
survival ratese0
Direction
highere0
Value
72.5%e0
Qualifier
59%e0
Comparator
Coratinae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Olea europaea
Olea europaea · TaxonHans Hillewaert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“During acclimatization, Kronaki also exhibited higher survival rates (72.5%) compared to Coratina (59%).”

✓ located verbatim in the source · abstract, characters 928–1032 · show in text ↑
e1

“Olive (Olea europaea L.)”

✓ located verbatim in the source · title, characters 57–81 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

ISSR analysis found 96% monomorphic bands in the propagated olive material.

Taxon
OliveOlea europaeae1
Outcome
monomorphic bandse0
Value
96%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Olea europaea
Olea europaea · TaxonHans Hillewaert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Genetic fidelity analysis using Inter-Simple Sequence Repeat (ISSR) markers indicated a high level of genetic stability, with 96% monomorphic bands and only 4% polymorphism observed.”

✓ located verbatim in the source · abstract, characters 1033–1215 · show in text ↑
e1

“Olive (Olea europaea L.)”

✓ located verbatim in the source · title, characters 57–81 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

ISSR analysis found 4% polymorphism in the propagated olive material.

Taxon
OliveOlea europaeae1
Outcome
polymorphisme0
Value
4%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Olea europaea
Olea europaea · TaxonHans Hillewaert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Genetic fidelity analysis using Inter-Simple Sequence Repeat (ISSR) markers indicated a high level of genetic stability, with 96% monomorphic bands and only 4% polymorphism observed.”

✓ located verbatim in the source · abstract, characters 1033–1215 · show in text ↑
e1

“Olive (Olea europaea L.)”

✓ located verbatim in the source · title, characters 57–81 · show in text ↑

in vitro · survival · Olea europaea (common olive)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
See all 3 papers →

in vitro · growth · Olea europaea (common olive)Positive and negative results

19 papers: 17 positive, 2 negative or no effect

16 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive

15 papers more in this group

See all 19 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.