Abstract
Invertases (INVs) included CWIN, CIN and VIN, are key enzymes in sucrose hydrolysis into glucose and fructose and essential for plant root development. Yet the effects of nitrogen and planting density on IbINVs expression remains unexplored in sweetpotato. This study identified 22 invertase (IbINV) genes in the sweetpotato genome and conducted comprehensive analyses of their subcellular localization, gene structure, and conserved motifs and domains. Gene Ontology functional and protein interaction network analysis suggested that IbCWIN1/2/5/6 potentially interact with HKL1/3, HXK2/3/4, and other proteins, significantly influencing carbohydrate metabolic functions and biological processes in sweetpotato. Transcriptome data revealed that IbCIN2 and IbVIN3 were highly expressed in fibrous root (FR) and potential storage root (PSR), while IbVIN4 exhibited high expression levels in storage roots (SR), and IbCWIN2 was highly expressed in both FR and SR. Moreover, the field experiments demonstrated that, compared with EN (180 kg N ha-1) combined with LD (50,000 plant ha− 1), MN (120 kg N ha− 1) combined with MD (62,500 plant ha− 1) enhanced storage roots number and weight. Notably, compared with LDEN treatment, IbCIN2, IbCWIN2, IbVIN3 and IbVIN4 under MDMN treatment were significantly upregulated, extremely significant differences at 15DAP, of which IbCIN2 showed a maximum of 23.24-fold change, showing a positive correlation with increased INV enzyme activity, glucose (GLc), and fructose (FRc) content. Additionally, screening of homologous genes showed that IbCIN2 is homologous to AtA/N-INVI and AtA/N-INVG, IbCWIN2, IbVIN3 and IbVIN4 are homologous to VACUOLAR, INVERTASE1 and BFRUCT4, with similar functions, further supporting their involvement in storage root formation and development. These observations underscore the expansion of IbINVs in sweetpotato and provide a theoretical basis for optimizing the interaction between nitrogen application and planting density in sweetpotato, allowing for more efficient nitrogen use and improved growth.
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Claims 12
Claim · model-written summary
The sweetpotato genome contains 22 identified invertase genes.
- Taxon
- sweetpotato
_Flower.jpg?width=60)
Ipomoea batatase0 - Gene
- invertase (IbINV)e0
- Outcome
- genese0
- Value
- 22e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Flower.jpg?width=500)
Verbatim
e0“identified 22 invertase (IbINV) genes in the sweetpotato genome”
Claim · model-written summary
Network analysis suggested IbCWIN1/2/5/6 potentially interact with HKL1/3 and HXK2/3/4.
- Taxon
- sweetpotatoIpomoea batatase1
- Gene
- IbCWIN1/2/5/6e0
- Outcome
- potentially interact with HKL1/3, HXK2/3/4e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IbCWIN1/2/5/6 potentially interact with HKL1/3, HXK2/3/4, and other proteins”
e1“sweetpotato genome”
Claim · model-written summary
IbCIN2 and IbVIN3 were highly expressed in fibrous and potential storage roots.
- Taxon
- sweetpotatoIpomoea batatase1
- Organ / stage
- fibrous root (FR) and potential storage root (PSR)e0
- Gene
- IbCIN2 and IbVIN3e0
- Outcome
- highly expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IbCIN2 and IbVIN3 were highly expressed in fibrous root (FR) and potential storage root (PSR)”
e1“sweetpotato genome”
Claim · model-written summary
IbVIN4 had high expression in storage roots.
- Taxon
- sweetpotatoIpomoea batatase1
- Organ / stage
- storage roots (SR)e0
- Gene
- IbVIN4e0
- Outcome
- high expression levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IbVIN4 exhibited high expression levels in storage roots (SR)”
e1“sweetpotato genome”
Claim · model-written summary
IbCWIN2 was highly expressed in fibrous and storage roots.
- Taxon
- sweetpotatoIpomoea batatase1
- Organ / stage
- FR and SRe0
- Gene
- IbCWIN2e0
- Outcome
- highly expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IbCWIN2 was highly expressed in both FR and SR”
e1“sweetpotato genome”
Claim · model-written summary
Medium nitrogen and density enhanced storage root number relative to high nitrogen and low density.
- Taxon
- sweetpotatoIpomoea batatase1
- Treatment
- MN (120 kg N ha− 1) combined with MD (62,500 plant ha− 1)e0
- Outcome
- storage roots numbere0
- Direction
- enhancede0
- Comparator
- EN (180 kg N ha-1) combined with LD (50,000 plant ha− 1)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“compared with EN (180 kg N ha-1) combined with LD (50,000 plant ha− 1), MN (120 kg N ha− 1) combined with MD (62,500 plant ha− 1) enhanced storage roots number and weight”
e1“sweetpotato genome”
Claim · model-written summary
Medium nitrogen and density enhanced storage root weight relative to high nitrogen and low density.
- Taxon
- sweetpotatoIpomoea batatase1
- Treatment
- MN (120 kg N ha− 1) combined with MD (62,500 plant ha− 1)e0
- Outcome
- weighte0
- Direction
- enhancede0
- Comparator
- EN (180 kg N ha-1) combined with LD (50,000 plant ha− 1)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“compared with EN (180 kg N ha-1) combined with LD (50,000 plant ha− 1), MN (120 kg N ha− 1) combined with MD (62,500 plant ha− 1) enhanced storage roots number and weight”
e1“sweetpotato genome”
Claim · model-written summary
IbCIN2, IbCWIN2, IbVIN3 and IbVIN4 were significantly upregulated under MDMN compared with LDEN.
- Taxon
- sweetpotatoIpomoea batatase1
- Treatment
- MDMN treatmente0
- Gene
- IbCIN2, IbCWIN2, IbVIN3 and IbVIN4e0
- Outcome
- upregulatede0
- Direction
- upregulatede0
- Qualifier
- 15DAPe0
- Comparator
- LDEN treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“compared with LDEN treatment, IbCIN2, IbCWIN2, IbVIN3 and IbVIN4 under MDMN treatment were significantly upregulated, extremely significant differences at 15DAP”
e1“sweetpotato genome”
Claim · model-written summary
IbCIN2 had a maximum 23.24-fold change.
- Taxon
- sweetpotatoIpomoea batatase1
- Gene
- IbCIN2e0
- Outcome
- fold changee0
- Direction
- maximume0
- Value
- 23.24-folde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IbCIN2 showed a maximum of 23.24-fold change”
e1“sweetpotato genome”
Claim · model-written summary
IbCIN2 expression correlated positively with increased invertase enzyme activity, glucose and fructose content.
- Taxon
- sweetpotatoIpomoea batatase1
- Gene
- IbCIN2e0
- Outcome
- INV enzyme activity, glucose (GLc), and fructose (FRc) contente0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IbCIN2 showed a maximum of 23.24-fold change, showing a positive correlation with increased INV enzyme activity, glucose (GLc), and fructose (FRc) content”
e1“sweetpotato genome”
Claim · model-written summary
IbCIN2 was identified as homologous to AtA/N-INVI and AtA/N-INVG.
- Taxon
- sweetpotatoIpomoea batatase1
- Gene
- IbCIN2e0
- Outcome
- homologous to AtA/N-INVI and AtA/N-INVGe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IbCIN2 is homologous to AtA/N-INVI and AtA/N-INVG”
e1“sweetpotato genome”
Claim · model-written summary
IbCWIN2, IbVIN3 and IbVIN4 were identified as homologous to VACUOLAR, INVERTASE1 and BFRUCT4.
- Taxon
- sweetpotatoIpomoea batatase1
- Gene
- IbCWIN2, IbVIN3 and IbVIN4e0
- Outcome
- homologous to VACUOLAR, INVERTASE1 and BFRUCT4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IbCWIN2, IbVIN3 and IbVIN4 are homologous to VACUOLAR, INVERTASE1 and BFRUCT4”
e1“sweetpotato genome”
The same questions in other papers
nitrogen · growth · Ipomoea batatas (sweet potato)Positive and negative results
18 papers in the same direction as this paper · 9 in the opposite direction
- no effect
- no effect
- negative
24 papers more in this group
See all 28 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.