Paper · 2024 · Industrial Crops and Products

Changes in morpho-physiological traits and phytochemical composition of Cannabis sativa L. treated with microbial biostimulants across different substrates

Dongmei Lyu; Eric D. Ruan; Rachel Backer; et al.

10
claims
23
verbatim quotes
3
citations
3
research areas
✓
Open access · cc-by

Abstract

Cannabis is a versatile crop with multiple uses, making its production highly significant. Its growth is influenced by various factors, including growing medium. Beneficial microbes, as a sustainable approach, can impact plant growth, but their effects on cannabis’ chemical profile are not well understood. This study examined two substrates (pure coco fibre and potting soil containing Sphagnum peat moss and perlite) and a microbial biostimulant comprising five Bacillus strains (two B. velezensis , two B. megaterium , and one B. licheniformis ) on cannabis growth and secondary metabolites across all growth stages. The results show that biostimulant inoculation increased flower yield by 16 % in coco fiber and 12 % in potting soil compared to uninoculated controls. It also enhanced CBDA (cannabidiolic acid) and THCA (tetrahydrocannabinolic acid) concentrations by 5 % and 10 % in coco fibre and potting soil, respectively. Biostimulant-treated plants showed a 5 % increase in total terpene levels in coco fibre but not in potting soil. Regarding growing medium, cannabis grown in potting soil showed greater overall biomass accumulation (leaf, stem, and flower) and higher leaf chlorophyll concentrations during both vegetative and reproductive stages. In contrast, coco fiber promoted root growth during the vegetative stage and resulted in significantly higher cannabinoid and terpene concentrations at maturity. Hence, coco fibre may be advantageous for enhancing cannabinoid and terpenoid profiles, while potting soil is better suited for maximizing plant yield, depending on the desired production goals. These findings emphasize the critical influence of substrate type and biostimulant application, and their interaction, in optimizing cannabis cultivation practices.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 10

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Biostimulant inoculation increased flower yield by 16 % in coco fiber.

Taxon
Cannabis sativa L.Cannabis sativat0
Treatment
biostimulant inoculatione0
Outcome
flower yielde0
Direction
increasede0
Value
16 %e0
Comparator
uninoculated controlse0
Setting
coco fibere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“biostimulant inoculation increased flower yield by 16 % in coco fiber and 12 % in potting soil compared to uninoculated controls.”

✓ located verbatim in the source · abstract, characters 643–772 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 72–90 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Biostimulant inoculation increased flower yield by 12 % in potting soil.

Taxon
Cannabis sativa L.Cannabis sativat0
Treatment
biostimulant inoculatione0
Outcome
flower yielde0
Direction
increasede0
Value
12 %e0
Comparator
uninoculated controlse0
Setting
potting soile0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“biostimulant inoculation increased flower yield by 16 % in coco fiber and 12 % in potting soil compared to uninoculated controls.”

✓ located verbatim in the source · abstract, characters 643–772 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 72–90 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Biostimulant inoculation enhanced CBDA and THCA concentrations by 5 % in coco fibre.

Taxon
Cannabis sativa L.Cannabis sativat0
Treatment
biostimulant inoculatione1
Outcome
CBDA (cannabidiolic acid) and THCA (tetrahydrocannabinolic acid) concentrationse0
Direction
enhancede0
Value
5 %e0
Setting
coco fibree0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“It also enhanced CBDA (cannabidiolic acid) and THCA (tetrahydrocannabinolic acid) concentrations by 5 % and 10 % in coco fibre and potting soil, respectively.”

✓ located verbatim in the source · abstract, characters 773–931 · show in text ↑
e1

“biostimulant inoculation increased flower yield”

✓ located verbatim in the source · abstract, characters 643–690 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 72–90 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Biostimulant inoculation enhanced CBDA and THCA concentrations by 10 % in potting soil.

Taxon
Cannabis sativa L.Cannabis sativat0
Treatment
biostimulant inoculatione1
Outcome
CBDA (cannabidiolic acid) and THCA (tetrahydrocannabinolic acid) concentrationse0
Direction
enhancede0
Value
10 %e0
Setting
potting soile0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“It also enhanced CBDA (cannabidiolic acid) and THCA (tetrahydrocannabinolic acid) concentrations by 5 % and 10 % in coco fibre and potting soil, respectively.”

✓ located verbatim in the source · abstract, characters 773–931 · show in text ↑
e1

“biostimulant inoculation increased flower yield”

✓ located verbatim in the source · abstract, characters 643–690 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 72–90 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Biostimulant-treated cannabis had 5 % higher total terpene levels in coco fibre.

Taxon
Cannabis sativa L.Cannabis sativat0
Treatment
Biostimulant-treatede0
Outcome
total terpene levelse0
Direction
increasee0
Value
5 %e0
Setting
coco fibree0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Biostimulant-treated plants showed a 5 % increase in total terpene levels in coco fibre but not in potting soil.”

✓ located verbatim in the source · abstract, characters 932–1044 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 72–90 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Biostimulant treatment did not increase total terpene levels in potting soil.

Taxon
Cannabis sativa L.Cannabis sativat0
Treatment
Biostimulant-treatede0
Outcome
total terpene levelse0
Setting
potting soile0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Biostimulant-treated plants showed a 5 % increase in total terpene levels in coco fibre but not in potting soil.”

✓ located verbatim in the source · abstract, characters 932–1044 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 72–90 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Potting soil produced greater overall leaf, stem, and flower biomass.

Taxon
Cannabis sativa L.Cannabis sativat0
Treatment
potting soile0
Outcome
biomass accumulation (leaf, stem, and flower)e0
Direction
greatere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“cannabis grown in potting soil showed greater overall biomass accumulation (leaf, stem, and flower)”

✓ located verbatim in the source · abstract, characters 1071–1170 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 72–90 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Potting soil produced higher leaf chlorophyll during vegetative and reproductive stages.

Taxon
Cannabis sativa L.Cannabis sativat0
Organ / stage
vegetative and reproductive stagese0
Treatment
potting soile1
Outcome
leaf chlorophyll concentrationse0
Direction
highere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“higher leaf chlorophyll concentrations during both vegetative and reproductive stages”

✓ located verbatim in the source · abstract, characters 1175–1260 · show in text ↑
e1

“cannabis grown in potting soil”

✓ located verbatim in the source · abstract, characters 1071–1101 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 72–90 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Coco fiber promoted root growth during the vegetative stage.

Taxon
Cannabis sativa L.Cannabis sativat0
Organ / stage
vegetative stagee0
Treatment
coco fibere0
Outcome
root growthe0
Direction
promotede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“coco fiber promoted root growth during the vegetative stage”

✓ located verbatim in the source · abstract, characters 1275–1334 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 72–90 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Coco fiber yielded significantly higher cannabinoid and terpene concentrations at maturity.

Taxon
Cannabis sativa L.Cannabis sativat0
Organ / stage
maturitye0
Treatment
coco fibere0
Outcome
cannabinoid and terpene concentrationse0
Direction
highere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“coco fiber promoted root growth during the vegetative stage and resulted in significantly higher cannabinoid and terpene concentrations at maturity”

✓ located verbatim in the source · abstract, characters 1275–1422 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 72–90 · show in text ↑

inoculation · yield · Cannabis sativaPositive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. no effect
See all 2 papers →

other · yield · Cannabis sativaPositive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
See all 3 papers →

other · molecular: metabolites · Cannabis sativaPositive and negative results

3 papers: 1 positive, 2 negative or no effect

2 in the opposite direction

  1. negative
  2. no effect
See all 3 papers →

growing medium · growth · Cannabis sativaPositive and negative results

9 papers: 5 positive, 4 negative or no effect

4 papers in the same direction as this paper · 4 in the opposite direction

  1. no effect
  2. no effect
  3. negative

5 papers more in this group

See all 9 papers →

growing medium · physiology: pigments · Cannabis sativaResults agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

inoculation · molecular: metabolites · Cannabis sativaResults agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.