Abstract
Background Chrysanthemum ( Chrysanthemum × morifolium Ramat.) is a particularly important autumn perennial flower for potted plant, flower bed and border, and cut flower with high ornamental value. However, abiotic stress can affect the ornamental quality of Chrysanthemum . NAC (NAM, ATAF1-2, and CUC2) transcription factors (TFs) play an important role in regulating plant growth and development, as well as responding to abiotic stresses. Methods In this study, the ClBRN1 ( Chrysanthemum lavandulifolium BEARSKIN gene) was isolated from the Chrysanthemum model plant C. lavandulifolium . And analyze the function of the gene through bioinformatics, subcellular localization and overexpression. Results Bioinformatics analysis showed that the ClBRN1 gene was a member of the NAC TFs family, with a CDS (coding sequence) length of 1,080 bp and encoding 359 amino acids. The subcellular localization results found that the gene was located in the nucleus and cell membrane. Furthermore, the transgenic results in Arabidopsis thaliana showed that the gene significantly reduces plant height while improving salt and low temperature tolerance. Observation of paraffin sections of Arabidopsis stems also revealed that the secondary cell wall of overexpressing Arabidopsis stems was significantly thicker than that of wild-type. The above results indicate that the ClBRN1 gene may play an important role in regulating plant resistance to abiotic stress. This study will provide new insights for molecular breeding of resistant chrysanthemums in the future.
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Claims 8
Claim · model-written summary
ClBRN1 belongs to the NAC transcription factor family.
- Taxon
- Chrysanthemum lavandulifolium
Chrysanthemum lavandulifoliumt0 - Gene
- ClBRN1e0
- Outcome
- member of the NAC TFs familye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ClBRN1 gene was a member of the NAC TFs family”
t0“Chrysanthemum lavandulifolium”
Claim · model-written summary
ClBRN1 has a CDS length of 1,080 bp.
- Taxon
- Chrysanthemum lavandulifoliumChrysanthemum lavandulifoliumt0
- Gene
- ClBRN1t0
- Outcome
- CDS (coding sequence) lengthe0
- Value
- 1,080 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with a CDS (coding sequence) length of 1,080 bp”
t0“ClBRN1 from Chrysanthemum lavandulifolium”
Claim · model-written summary
ClBRN1 encodes 359 amino acids.
- Taxon
- Chrysanthemum lavandulifoliumChrysanthemum lavandulifoliumt0
- Gene
- ClBRN1t0
- Outcome
- amino acidse0
- Value
- 359 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“encoding 359 amino acids”
t0“ClBRN1 from Chrysanthemum lavandulifolium”
Claim · model-written summary
ClBRN1 localized to the nucleus and cell membrane.
- Taxon
- Chrysanthemum lavandulifoliumChrysanthemum lavandulifoliumt0
- Gene
- ClBRN1t0
- Outcome
- located in the nucleus and cell membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the gene was located in the nucleus and cell membrane”
t0“ClBRN1 from Chrysanthemum lavandulifolium”
Claim · model-written summary
ClBRN1 transgenics were significantly shorter.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the gene significantly reduces plant height”
t0“ClBRN1 from Chrysanthemum lavandulifolium enhances the stress resistance of transgenic Arabidopsis”
Claim · model-written summary
ClBRN1 improved salt tolerance in Arabidopsis.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“improving salt and low temperature tolerance”
t0“ClBRN1 from Chrysanthemum lavandulifolium enhances the stress resistance of transgenic Arabidopsis”
Claim · model-written summary
ClBRN1 improved low-temperature tolerance in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- ClBRN1t0
- Gene
- ClBRN1t0
- Outcome
- low temperature tolerancee0
- Direction
- improvinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“improving salt and low temperature tolerance”
t0“ClBRN1 from Chrysanthemum lavandulifolium enhances the stress resistance of transgenic Arabidopsis”
Claim · model-written summary
Overexpressing Arabidopsis stems had significantly thicker secondary cell walls than wild type.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- stemse0
- Treatment
- overexpressinge0
- Outcome
- secondary cell walle0
- Direction
- thickere0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the secondary cell wall of overexpressing Arabidopsis stems was significantly thicker than that of wild-type”