Abstract
Introduction Cauliflower is widely cultivated all over the world is attributed to its palatable flavor, high levels of anti-cancer compounds, and diverse array of nutrients. Exposure to extremely cold stress during production can result in a more frequent occurrence of purple discoloration in cauliflower curds. In response to cold stress, plants naturally produce anthocyanins to eliminate reactive oxygen species (ROS) generated as a defense mechanism. Methods This research involved conducting mRNA sequencing analysis on cauliflower curds both before and after exposure to cold stress treatment. Results It was determined that the up-regulation of anthocyanin biosynthesis-related genes CHS, CHI, DFR, ANS, UGFT, PAP1/2 , and MYBL2 occurred significantly in response to cold stress, resulting in a significant increase in total anthocyanin content. Subsequently, miRNA sequencing was employed to identify miRNAs in cauliflower curds, followed by differential expression analysis. The results showed that Bna-miR289 and Ath-miR157a may play a key role in regulating the accumulation of anthocyanin in cauliflower curds. Furthermore, we utilized degradome sequencing data to predict the target genes of the identified miRNAs, resulting in the identification of BolK_3g48940.1, BolK_9g11680.1, BolK_7g41780.1, BolK_3g68050.1, and BolK_3g729700.1 as targets. Subsequently, the expression patterns of the miRNAs and their target genes were validated using qRT-PCR, the results showed that Ath-miR157a and its target genes BolK_3g68050.1 and BolK_3g72970.1 may be the key to the purple of cauliflower curds under cold stress. Discussion Our preliminary findings identified key miRNAs and their target genes that may be involved in regulating anthocyanin synthesis, thereby enhancing the cold tolerance of cauliflower through mRNA, miRNA, and degradome sequencing. Overall, our study sheds light on the activation of anthocyanin synthesis in flower curds under cold stress conditions as a mechanism to enhance resilience to adverse environmental conditions.
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Claims 7
Claim · model-written summary
Cold stress significantly upregulated anthocyanin biosynthesis genes CHS, CHI, DFR, ANS, UGFT, PAP1/2, and MYBL2.
- Taxon
- Cauliflower
Brassica oleracea var. botrytist0 - Treatment
- cold stresse0
- Gene
- CHS, CHI, DFR, ANS, UGFT, PAP1/2 , and MYBL2e0
- Outcome
- up-regulation of anthocyanin biosynthesis-related genese0
- Direction
- up-regulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the up-regulation of anthocyanin biosynthesis-related genes CHS, CHI, DFR, ANS, UGFT, PAP1/2 , and MYBL2 occurred significantly in response to cold stress”
t0“Cauliflower”
Claim · model-written summary
Cold stress significantly increased total anthocyanin content.
- Taxon
- CauliflowerBrassica oleracea var. botrytist0
- Outcome
- total anthocyanin contente0
- Direction
- increasee0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resulting in a significant increase in total anthocyanin content”
t0“Cauliflower”
Claim · model-written summary
Bna-miR289 may regulate anthocyanin accumulation in cauliflower curds.
- Taxon
- cauliflowerBrassica oleracea var. botrytise0
- Organ / stage
- cauliflower curdse0
- Gene
- Bna-miR289e0
- Outcome
- accumulation of anthocyanine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bna-miR289 and Ath-miR157a may play a key role in regulating the accumulation of anthocyanin in cauliflower curds”
Claim · model-written summary
Ath-miR157a may regulate anthocyanin accumulation in cauliflower curds.
- Taxon
- cauliflowerBrassica oleracea var. botrytise0
- Organ / stage
- cauliflower curdse0
- Gene
- Ath-miR157ae0
- Outcome
- accumulation of anthocyanine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bna-miR289 and Ath-miR157a may play a key role in regulating the accumulation of anthocyanin in cauliflower curds”
Claim · model-written summary
Degradome analysis identified BolK_3g48940.1, BolK_9g11680.1, and BolK_7g41780.1 as miRNA targets.
- Taxon
- CauliflowerBrassica oleracea var. botrytist0
- Gene
- BolK_3g48940.1, BolK_9g11680.1, BolK_7g41780.1e0
- Outcome
- targetse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identification of BolK_3g48940.1, BolK_9g11680.1, BolK_7g41780.1, BolK_3g68050.1, and BolK_3g729700.1 as targets”
t0“Cauliflower”
Claim · model-written summary
Degradome analysis identified BolK_3g68050.1 and BolK_3g729700.1 as miRNA targets.
- Taxon
- CauliflowerBrassica oleracea var. botrytist0
- Gene
- BolK_3g68050.1, and BolK_3g729700.1e0
- Outcome
- targetse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identification of BolK_3g48940.1, BolK_9g11680.1, BolK_7g41780.1, BolK_3g68050.1, and BolK_3g729700.1 as targets”
t0“Cauliflower”
Claim · model-written summary
Ath-miR157a and its targets BolK_3g68050.1 and BolK_3g72970.1 may be key to purple cauliflower curds under cold stress.
- Taxon
- cauliflowerBrassica oleracea var. botrytise0
- Treatment
- cold stresse0
- Gene
- Ath-miR157a and its target genes BolK_3g68050.1 and BolK_3g72970.1e0
- Outcome
- purple of cauliflower curdse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ath-miR157a and its target genes BolK_3g68050.1 and BolK_3g72970.1 may be the key to the purple of cauliflower curds under cold stress”
The same questions in other papers
cold · molecular: expression · Brassica oleracea (wild cabbage)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.