Abstract
CELL DIVISION CYCLE 5 (CDC5) is a R2R3-type MYB transcription factor, serving as a key component of modifier of snc1, 4-associated complex/NineTeen complex, which is associated with plant immunity, RNA splicing, and miRNA biogenesis. In this study, we demonstrate that mutation of CDC5 accelerates flowering in Arabidopsis (Arabidopsis thaliana). CDC5 activates the expression of FLOWERING LOCUS C (FLC) by binding to and affecting the enrichment of RNA polymerase II on FLC chromatin. Moreover, genetic analysis confirmed that CDC5 regulates flowering in an FLC-dependent manner. Furthermore, we characterized the interaction of CDC5 with the RNA polymerase-associated factor 1 (Paf1) complex and confirmed that CDC5, as part of the spliceosome, mediates genome-wide alternative splicing, as revealed by RNA-seq. CDC5 affected the splicing of flowering-associated genes such as FLC, SEF, and MAFs. Additionally, we also demonstrated that CDC5 contributes to the regulation of histone modification of FLC chromatin, which further promotes FLC expression. In summary, our results establish CDC5 as a key factor regulating flowering. This provides valuable insight for future research into plant flowering.
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Claims 7
Claim · model-written summary
CDC5 mutation accelerates flowering in Arabidopsis.
- Taxon
- Arabidopsis (Arabidopsis thaliana)
Arabidopsis thalianae0 - Gene
- CDC5e0
- Outcome
- floweringe0
- Direction
- acceleratese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutation of CDC5 accelerates flowering in Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
CDC5 activates FLC expression through binding and RNA polymerase II enrichment on FLC chromatin.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CDC5e0
- Outcome
- expression of FLOWERING LOCUS C (FLC)e0
- Direction
- activatese0
- Qualifier
- binding to and affecting the enrichment of RNA polymerase II on FLC chromatine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CDC5 activates the expression of FLOWERING LOCUS C (FLC) by binding to and affecting the enrichment of RNA polymerase II on FLC chromatin”
t0“Arabidopsis”
Claim · model-written summary
CDC5 regulates flowering in an FLC-dependent manner.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CDC5e0
- Outcome
- floweringe0
- Qualifier
- FLC-dependent mannere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genetic analysis confirmed that CDC5 regulates flowering in an FLC-dependent manner”
t0“Arabidopsis”
Claim · model-written summary
CDC5 interacts with the Paf1 complex.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CDC5e0
- Outcome
- interaction of CDC5 with the RNA polymerase-associated factor 1 (Paf1) complexe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“interaction of CDC5 with the RNA polymerase-associated factor 1 (Paf1) complex”
t0“Arabidopsis”
Claim · model-written summary
CDC5 mediates genome-wide alternative splicing as part of the spliceosome.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CDC5e0
- Outcome
- genome-wide alternative splicinge0
- Qualifier
- as part of the spliceosomee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CDC5, as part of the spliceosome, mediates genome-wide alternative splicing, as revealed by RNA-seq”
t0“Arabidopsis”
Claim · model-written summary
CDC5 affects splicing of FLC, SEF, and MAFs.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CDC5e0
- Outcome
- splicing of flowering-associated genes such as FLC, SEF, and MAFse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CDC5 affected the splicing of flowering-associated genes such as FLC, SEF, and MAFs”
t0“Arabidopsis”
Claim · model-written summary
CDC5 contributes to histone modification of FLC chromatin, promoting FLC expression.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CDC5e0
- Outcome
- FLC expressione0
- Direction
- promotese0
- Qualifier
- histone modification of FLC chromatine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CDC5 contributes to the regulation of histone modification of FLC chromatin, which further promotes FLC expression”
t0“Arabidopsis”