Abstract
Caffeoyl coenzyme A-O-methyltransferase (CCoAOMT) has a critical function in the lignin biosynthesis pathway. However, its functions in cotton are not clear. In this research, we observed 50 CCoAOMT genes from four cotton species, including two diploids (Gossypium arboretum, 9, and Gossypium raimondii, 8) and two tetraploids (Gossypium hirsutum, 16, and Gossypium barbadense, 17), performed bioinformatic analysis, and focused on the involvement and functions of GhCCoAOMT7 in lignin synthesis of Gossypium hirsutum. CCoAOMT proteins were divided into four subgroups based on the phylogenetic tree analysis. Motif analysis revealed that all CCoAOMT proteins possess conserved Methyltransf_3 domains, and conserved structural features were identified based on the genes’ exon-intron organization. A synteny analysis suggested that segmental duplications were the primary cause in the expansion of the CCoAOMT genes family. Transcriptomic data analysis of GhCCoAOMTs revealed that GhCCoAOMT2, GhCCoAOMT7, and GhCCoAOMT14 were highly expressed in stems. Subcellular localization experiments of GhCCoAOMT2, GhCCoAOMT7, and GhCCoAOMT14 showed that GhCCoAOMT2, GhCCoAOMT7, and GhCCoAOMT14 were localized in the nucleus and plasma membrane. However, there are no cis-regulatory elements related to lignin synthesis in the GhCCoAOMT7 gene promoter. GhCCoAOMT7 expression was inhibited by virus-induced gene silencing technology to obtain gene silencing lines, the suppression of GhCCoAOMT7 expression resulted in a 56% reduction in the lignin content in cotton stems, and the phloroglucinol staining area corresponding to the xylem was significantly decreased, indicating that GhCCoAOMT7 positively regulates lignin synthesis. Our results provided fundamental information regarding CCoAOMTs and highlighted their potential functions in cotton lignin biosynthesis and lignification.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Fifty CCoAOMT genes were identified from four cotton species.
- Taxon
- cotton species
Gossypium hirsutume1 - Gene
- CCoAOMTe1
- Outcome
- CCoAOMT genese1
- Value
- 50e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we observed 50 CCoAOMT genes from four cotton species”
Claim · model-written summary
GhCCoAOMT2, GhCCoAOMT7 and GhCCoAOMT14 are highly expressed in cotton stems.
- Taxon
- CottonGossypium hirsutumt0
- Organ / stage
- stemse1
- Gene
- GhCCoAOMT2, GhCCoAOMT7, and GhCCoAOMT14e1
- Outcome
- highly expressede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Transcriptomic data analysis of GhCCoAOMTs revealed that GhCCoAOMT2, GhCCoAOMT7, and GhCCoAOMT14 were highly expressed in stems”
t0“Lignin Synthesis in Cotton”
Claim · model-written summary
GhCCoAOMT2, GhCCoAOMT7 and GhCCoAOMT14 localize to the nucleus and plasma membrane.
- Taxon
- CottonGossypium hirsutumt0
- Gene
- GhCCoAOMT2, GhCCoAOMT7, and GhCCoAOMT14e1
- Outcome
- Subcellular localizatione1
- Qualifier
- localized in the nucleus and plasma membranee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Subcellular localization experiments of GhCCoAOMT2, GhCCoAOMT7, and GhCCoAOMT14 showed that GhCCoAOMT2, GhCCoAOMT7, and GhCCoAOMT14 were localized in the nucleus and plasma membrane”
t0“Lignin Synthesis in Cotton”
Claim · model-written summary
The GhCCoAOMT7 promoter has no cis-regulatory elements related to lignin synthesis.
- Taxon
- CottonGossypium hirsutumt0
- Gene
- GhCCoAOMT7e1
- Outcome
- cis-regulatory elements related to lignin synthesise1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“there are no cis-regulatory elements related to lignin synthesis in the GhCCoAOMT7 gene promoter”
t0“Lignin Synthesis in Cotton”
Claim · model-written summary
Virus-induced silencing of GhCCoAOMT7 reduced lignin content in cotton stems by 56%.
- Taxon
- cottonGossypium hirsutume2
- Organ / stage
- stemse2
- Treatment
- virus-induced gene silencing technologye1
- Gene
- GhCCoAOMT7e2
- Outcome
- lignin contente2
- Direction
- reductione2
- Value
- 56%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“GhCCoAOMT7 expression was inhibited by virus-induced gene silencing technology to obtain gene silencing lines”
e2“the suppression of GhCCoAOMT7 expression resulted in a 56% reduction in the lignin content in cotton stems”
Claim · model-written summary
GhCCoAOMT7 positively regulates lignin synthesis in cotton.
- Taxon
- CottonGossypium hirsutumt0
- Gene
- GhCCoAOMT7e1
- Outcome
- lignin synthesise1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“indicating that GhCCoAOMT7 positively regulates lignin synthesis”
t0“Lignin Synthesis in Cotton”
Claim · model-written summary
Phloroglucinol staining of the xylem area was significantly decreased in GhCCoAOMT7-silenced cotton.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- virus-induced gene silencing technologye1
- Outcome
- phloroglucinol staining area corresponding to the xyleme2
- Direction
- decreasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“GhCCoAOMT7 expression was inhibited by virus-induced gene silencing technology to obtain gene silencing lines”
e2“the phloroglucinol staining area corresponding to the xylem was significantly decreased”
t0“Lignin Synthesis in Cotton”
Claim · model-written summary
Segmental duplications were the primary cause of CCoAOMT gene family expansion.
- Taxon
- CottonGossypium hirsutumt0
- Outcome
- the expansion of the CCoAOMT genes familye1
- Qualifier
- segmental duplicationse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A synteny analysis suggested that segmental duplications were the primary cause in the expansion of the CCoAOMT genes family”
t0“Lignin Synthesis in Cotton”
Claim · model-written summary
All CCoAOMT proteins possess conserved Methyltransf_3 domains.
- Taxon
- CottonGossypium hirsutumt0
- Outcome
- conserved Methyltransf_3 domainse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Motif analysis revealed that all CCoAOMT proteins possess conserved Methyltransf_3 domains”
t0“Lignin Synthesis in Cotton”
Claim · model-written summary
CCoAOMT proteins fall into four phylogenetic subgroups.
- Taxon
- CottonGossypium hirsutumt0
- Outcome
- CCoAOMT proteinse1
- Value
- four subgroupse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“CCoAOMT proteins were divided into four subgroups based on the phylogenetic tree analysis”
t0“Lignin Synthesis in Cotton”