Abstract
Low temperatures greatly restrict the development, growth, and productivity of soybeans, with their effects differing across various cultivars. The present work investigated the transcriptome and physiological reactions of two soybean cultivars, namely "KD52" exhibiting cold tolerance and "DS17" displaying cold sensitivity, to cold stress across a precisely defined period. The soybean plants were subjected to cold treatment at 6 °C for durations of 0, 2, 4, and 8 h. A comparative physiological marker study revealed distinct reactions to cold stress in the two cultivars. The findings showed that increased malondialdehyde levels provided evidence of DS17's heightened vulnerability to lipid peroxidation and membrane degradation. In contrast, the KD52 cultivar exhibited increased activities of antioxidant enzymes, including peroxidase and superoxide dismutase, in response to cold exposure, suggesting a strong antioxidant defense system against oxidative stress. The transcriptomic analysis revealed dynamic responses, mapping 54,532 genes. Within this group, a total of 234 differentially expressed genes (DEGs) were found to be consistently changed at several time intervals, showing unique expression patterns across the two cultivars. Analysis of the association between these important DEGs and the physiological indicators revealed candidate genes that may be involved in controlling oxidative damage and antioxidant defenses. Some key genes showed a progressive rise in expression over time in both cultivars, with a more significant acceleration in KD52, and are probably involved in promoting adaptation processes during extended periods of cold exposure. The identification of improved defense mechanisms in KD52, together with the identification of crucial genes, offers great prospects for enhancing the cold stress resilience of soybean.
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Claims 6
Claim · model-written summary
Cold-sensitive DS17 showed increased malondialdehyde and lipid peroxidation vulnerability.
- Taxon
- Soybean
Glycine maxt0 - Material
- DS17e0
- Outcome
- malondialdehyde levelse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased malondialdehyde levels provided evidence of DS17's heightened vulnerability to lipid peroxidation and membrane degradation”
t0“Soybean”
Claim · model-written summary
KD52 increased peroxidase and superoxide dismutase activities after cold exposure.
- Taxon
- SoybeanGlycine maxt0
- Material
- KD52e0
- Treatment
- cold exposuree0
- Gene
- peroxidase and superoxide dismutasee0
- Outcome
- activities of antioxidant enzymes, including peroxidase and superoxide dismutasee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the KD52 cultivar exhibited increased activities of antioxidant enzymes, including peroxidase and superoxide dismutase, in response to cold exposure”
t0“Soybean”
Claim · model-written summary
Transcriptomic analysis mapped 54,532 soybean genes.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- genese0
- Value
- 54,532 genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcriptomic analysis revealed dynamic responses, mapping 54,532 genes”
t0“Soybean”
Claim · model-written summary
Two cultivars had 234 consistently changed DEGs across intervals with distinct expression patterns.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- differentially expressed genes (DEGs)e0
- Value
- 234 differentially expressed genese0
- Qualifier
- at several time intervalse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“234 differentially expressed genes (DEGs) were found to be consistently changed at several time intervals, showing unique expression patterns across the two cultivars”
t0“Soybean”
Claim · model-written summary
DEG–physiology associations identified candidate genes for oxidative damage and antioxidant defense.
- Taxon
- SoybeanGlycine maxt0
- Gene
- DEGse0
- Outcome
- candidate genese0
- Qualifier
- may be involved in controlling oxidative damage and antioxidant defensese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of the association between these important DEGs and the physiological indicators revealed candidate genes that may be involved in controlling oxidative damage and antioxidant defenses”
t0“Soybean”
Claim · model-written summary
Some key genes rose progressively in both cultivars, with greater acceleration in KD52.
- Taxon
- SoybeanGlycine maxt0
- Material
- KD52e0
- Outcome
- expressione0
- Direction
- progressive risee0
- Qualifier
- in both cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some key genes showed a progressive rise in expression over time in both cultivars, with a more significant acceleration in KD52”
t0“Soybean”
The same questions in other papers
cold · physiology: antioxidant enzymes · Glycine max (soybean)Positive and negative results
2 in the opposite direction
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.