Abstract
Changes in root system architecture are vital for plant adaptation to drought stress, yet the underlying molecular mechanisms of this process remain largely elusive. Here, FUSCA3 (FUS3), a B3 domain transcription factor isolated from Populus euphratica, was found to be an important gene of regulating lateral root (LR) development under drought stress. The expression of PeFUS3 was strongly induced by ABA and dehydration treatments. Overexpressing PeFUS3 in poplar 84 K (P. alba × P. glandulosa) positively regulated LR growth and enhanced drought tolerance, while the knockout lines, generated by the CRISPR/Cas9 system, displayed repressed LR growth and weakened drought tolerance. Further investigation demonstrated that PeFUS3 activated the expression of PIN2, PIN6a and AUX1, which were key genes involved in auxin transport, suggesting PeFUS3 modulated LR development under drought stress through auxin signalling. Moreover, PeFUS3 directly upregulated PePYL3 expression, and overexpressing PePYL3 poplar lines exhibited significantly increased drought resistance. In addition, PeABF2, an ABA responsive transcription factor, interacted with PeFUS3 and activated its transcription, indicating PeFUS3 was involved in ABA signalling pathway. Taken together, PeFUS3 is a key regulator, maintaining root growth of poplar by modulating the crosstalk of auxin and ABA signalling under drought stress.
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Claims 11
Claim · model-written summary
ABA and dehydration strongly induced PeFUS3 expression.
- Treatment
- ABA and dehydration treatmentse0
- Gene
- PeFUS3e0
- Outcome
- expression of PeFUS3e0
- Direction
- strongly inducede0
PopulusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of PeFUS3 was strongly induced by ABA and dehydration treatments.”
t0“Populus”
Claim · model-written summary
PeFUS3 overexpression increased lateral root growth in poplar 84 K.
- Taxon
- poplar 84 Ke0
- Material
- poplar 84 Ke0
- Treatment
- Overexpressing PeFUS3e0
- Gene
- PeFUS3e0
- Outcome
- LR growthe0
- Direction
- positively regulatede0
Not reportedComparator
Verbatim
e0“Overexpressing PeFUS3 in poplar 84 K (P. alba × P. glandulosa) positively regulated LR growth and enhanced drought tolerance”
Claim · model-written summary
PeFUS3 overexpression enhanced drought tolerance in poplar 84 K.
- Taxon
- poplar 84 Ke0
- Material
- poplar 84 Ke0
- Treatment
- Overexpressing PeFUS3e0
- Gene
- PeFUS3e0
- Outcome
- drought tolerancee0
- Direction
- enhancede0
Not reportedComparator
Verbatim
e0“Overexpressing PeFUS3 in poplar 84 K (P. alba × P. glandulosa) positively regulated LR growth and enhanced drought tolerance”
Claim · model-written summary
PeFUS3 knockout repressed lateral root growth.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the knockout lines, generated by the CRISPR/Cas9 system, displayed repressed LR growth and weakened drought tolerance.”
e1“Overexpressing PeFUS3 in poplar 84 K (P. alba × P. glandulosa)”
Claim · model-written summary
PeFUS3 knockout weakened drought tolerance.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the knockout lines, generated by the CRISPR/Cas9 system, displayed repressed LR growth and weakened drought tolerance.”
e1“Overexpressing PeFUS3 in poplar 84 K (P. alba × P. glandulosa)”
Claim · model-written summary
PeFUS3 activated PIN2 expression.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PeFUS3 activated the expression of PIN2, PIN6a and AUX1”
t0“Populus”
Claim · model-written summary
PeFUS3 activated PIN6a expression.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PeFUS3 activated the expression of PIN2, PIN6a and AUX1”
t0“Populus”
Claim · model-written summary
PeFUS3 activated AUX1 expression.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PeFUS3 activated the expression of PIN2, PIN6a and AUX1”
t0“Populus”
Claim · model-written summary
PeFUS3 directly upregulated PePYL3 expression.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PeFUS3 directly upregulated PePYL3 expression”
t0“Populus”
Claim · model-written summary
PePYL3 overexpression significantly increased poplar drought resistance.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpressing PePYL3 poplar lines exhibited significantly increased drought resistance.”
Claim · model-written summary
PeABF2 interacted with PeFUS3 and activated its transcription.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PeABF2, an ABA responsive transcription factor, interacted with PeFUS3 and activated its transcription”
t0“Populus”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Populus (poplar)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
drought · molecular: expression · Populus (poplar)Positive and negative results
6 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- no effect
- negative
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.