Abstract
Aims: Anthurium (Anthurium andraeanum Lind.) is a valued ornamental plant, but efficient mass propagation methods are lacking. This study investigated the impact of copper sulfate (CuSO4) on its in vitro propagation efficiency. Study Design: Complete Randomized Design (CRD). Place and Duration of Study: Faculty of Agricultural Sciences, Sabaragamuwa University of Sri Lanka, between November 2023 and June 2024. Methodology: Callus induction and shoot regeneration were assessed using various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), Benzyladenine (BA), and CuSO4. Callus induction from sterilized leaf explants and callus proliferation were conducted using Murashige and Skoog (MS) basal medium supplemented with concentrations of 2,4-D, BA, and CuSO4 in the dark. Shoot regeneration from the calli was performed on a modified MS medium with different concentrations of growth regulators and CuSO4 under light. Regenerated shoots were then transferred to a medium enriched with CuSO4 for rooting, and the survival rate of the acclimatized plants was assessed. Results: The results show CuSO4 significantly enhanced callus induction and shoot regeneration rates. The optimal medium for callus induction consisted of MS medium with 0.3 mg/L 2,4-D, 1.0 mg/L BA, and 5.0 mg/L CuSO4, leading to rapid induction (46 days) and a high induction percentage (86.67%) of calli. For shoot regeneration, the most effective medium contained 0.1 mg/L 2,4-D, 0.75 mg/L BA, and 5.0 mg/L CuSO4, showing superior regeneration efficiency in total shoot number and average shoot length. The success of root initiation and acclimatization improved notably in plants derived from CuSO4 treated media, with a survival rate of 92%. Conclusion: This study highlights the significant role of CuSO4 in enhancing the in vitro propagation of Anthurium andraeanum Lind., with optimal callus induction and shoot regeneration observed at specific concentrations. The inclusion of CuSO4 led to rapid callus formation, superior shoot regeneration, and a high survival rate during acclimatization.
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Claims 6
Claim · model-written summary
CuSO4 significantly enhanced callus induction and shoot regeneration.
AnthuriumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CuSO4 significantly enhanced callus induction and shoot regeneration rates”
t0“Anthurium (Anthurium andraeanum Lind.)”
Claim · model-written summary
The optimal callus induction medium produced calli in 46 days.
- Treatment
- MS medium with 0.3 mg/L 2,4-D, 1.0 mg/L BA, and 5.0 mg/L CuSO4e0
- Outcome
- rapid inductione0
- Direction
- rapide0
- Value
- 46 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimal medium for callus induction consisted of MS medium with 0.3 mg/L 2,4-D, 1.0 mg/L BA, and 5.0 mg/L CuSO4, leading to rapid induction (46 days) and a high induction percentage (86.67%) of calli”
t0“Anthurium (Anthurium andraeanum Lind.)”
Claim · model-written summary
The optimal callus induction medium achieved 86.67% callus induction.
- Treatment
- MS medium with 0.3 mg/L 2,4-D, 1.0 mg/L BA, and 5.0 mg/L CuSO4e0
- Outcome
- induction percentagee0
- Direction
- highe0
- Value
- 86.67%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimal medium for callus induction consisted of MS medium with 0.3 mg/L 2,4-D, 1.0 mg/L BA, and 5.0 mg/L CuSO4, leading to rapid induction (46 days) and a high induction percentage (86.67%) of calli”
t0“Anthurium (Anthurium andraeanum Lind.)”
Claim · model-written summary
A medium containing 0.1 mg/L 2,4-D, 0.75 mg/L BA and 5.0 mg/L CuSO4 gave superior total shoot number and average shoot length.
- Treatment
- 0.1 mg/L 2,4-D, 0.75 mg/L BA, and 5.0 mg/L CuSO4e0
- Outcome
- total shoot number and average shoot lengthe0
- Direction
- superiore0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the most effective medium contained 0.1 mg/L 2,4-D, 0.75 mg/L BA, and 5.0 mg/L CuSO4, showing superior regeneration efficiency in total shoot number and average shoot length”
t0“Anthurium (Anthurium andraeanum Lind.)”
Claim · model-written summary
Root initiation improved in plants derived from CuSO4-treated media.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The success of root initiation and acclimatization improved notably in plants derived from CuSO4 treated media, with a survival rate of 92%”
t0“Anthurium (Anthurium andraeanum Lind.)”
Claim · model-written summary
Acclimatization survival reached 92% for plants derived from CuSO4-treated media.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The success of root initiation and acclimatization improved notably in plants derived from CuSO4 treated media, with a survival rate of 92%”
t0“Anthurium (Anthurium andraeanum Lind.)”
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.