Abstract
Abstract Xanthomonas campestris pv. campestris ( Xcc ) is the causal agent of black rot in Brassica vegetables, which can induce the host plant to produce salicylic acid and 4-hydroxybenzoic acid (4-HBA) during infection. Xcc was previously shown to sense and degrade host plant-derived 4-HBA via the sensor PobR and a PobA-dependent pathway. The degradation of 4-HBA is associated with Xcc virulence in cabbage. The present study generated a reporter strain XC1::P pobA - gusA to monitor pobA transcription. 4-HBA-like compounds were screened for their ability to interfere with pobA transcription. Benzoic acid (BA) was found to efficiently decrease pobA transcription in a dose-dependent manner. Xcc neither produced nor degraded BA; however, the exogenous addition of BA to the 4-HBA-containing Xcc culture significantly decreased the 4-HBA degradation rate. Furthermore, addition of BA into the Xcc culture did not significantly affect the transcription of pobA or pobR ; however, addition of BA into the 4-HBA-containing culture significantly decreased the transcription of both genes. Isothermal titration calorimetry and an electrophoretic mobility shift assay revealed that BA binds to PobR with a moderate affinity, which interfered with the binding of 4-HBA/PobR complex to the pobA promoter and thereby inhibiting pobA transcription and 4-HBA degradation. The endogenous BA level of the infected cabbage leaves increased in response to Xcc infection. In the presence of BA, the virulence of Xcc on cabbage decreased significantly. Taken together, these results suggest that cabbage utilizes BA to interfere with 4-HBA degradation, thereby reducing Xcc virulence. Thus, BA has the potential to be developed as a bactericide against Xcc infection.
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Claims 8
Claim · model-written summary
Benzoic acid decreased pobA transcription with dose.
- Taxon
- cabbage
Brassica oleracea var. capitatae1 - Treatment
- Benzoic acid (BA)e0
- Interactor
- Xanthomonas campestris
_(25868012152).jpg?width=60)
Xanthomonas campestrist0pathogen - Gene
- pobAe0
- Outcome
- pobA transcriptione0
- Direction
- decreasee0
- Qualifier
- dose-dependent mannere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(25868012152).jpg?width=500)
Verbatim
e0“Benzoic acid (BA) was found to efficiently decrease pobA transcription in a dose-dependent manner.”
e1“virulence in cabbage”
t0“Xanthomonas campestris”
Claim · model-written summary
Xcc neither produced nor degraded BA.
- Taxon
- cabbageBrassica oleracea var. capitatae1
- Outcome
- Xcc neither produced nor degraded BAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Xcc neither produced nor degraded BA;”
e1“virulence in cabbage”
t0“Xanthomonas campestris”
Claim · model-written summary
BA addition decreased 4-HBA degradation in Xcc culture.
- Taxon
- cabbageBrassica oleracea var. capitatae1
- Treatment
- exogenous addition of BAe0
- Outcome
- 4-HBA degradation ratee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the exogenous addition of BA to the 4-HBA-containing Xcc culture significantly decreased the 4-HBA degradation rate.”
e1“virulence in cabbage”
t0“Xanthomonas campestris”
Claim · model-written summary
BA alone did not significantly affect pobA or pobR transcription.
- Taxon
- cabbageBrassica oleracea var. capitatae1
- Treatment
- addition of BAe0
- Outcome
- transcription of pobA or pobRe0
- Direction
- did not significantly affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“addition of BA into the Xcc culture did not significantly affect the transcription of pobA or pobR”
e1“virulence in cabbage”
t0“Xanthomonas campestris”
Claim · model-written summary
BA in 4-HBA culture decreased both pobA and pobR transcription.
- Taxon
- cabbageBrassica oleracea var. capitatae1
- Treatment
- addition of BAe0
- Interactor
- Xanthomonas campestrisXanthomonas campestrist0pathogen
- Outcome
- transcription of both genese0
- Direction
- decreasede0
- Qualifier
- 4-HBA-containing culturee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“addition of BA into the 4-HBA-containing culture significantly decreased the transcription of both genes.”
e1“virulence in cabbage”
t0“Xanthomonas campestris”
Claim · model-written summary
BA binds PobR and interferes with 4-HBA/PobR promoter binding, inhibiting pobA transcription and 4-HBA degradation.
- Taxon
- cabbageBrassica oleracea var. capitatae1
- Interactor
- Xanthomonas campestrisXanthomonas campestrist0pathogen
- Gene
- PobRe0
- Compound
- BAe0
- Outcome
- inhibiting pobA transcription and 4-HBA degradatione0
- Qualifier
- interfered with the binding of 4-HBA/PobR complex to the pobA promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BA binds to PobR with a moderate affinity, which interfered with the binding of 4-HBA/PobR complex to the pobA promoter and thereby inhibiting pobA transcription and 4-HBA degradation.”
e1“virulence in cabbage”
t0“Xanthomonas campestris”
Claim · model-written summary
Endogenous BA increased in infected cabbage leaves after Xcc infection.
- Taxon
- cabbageBrassica oleracea var. capitatae0
- Organ / stage
- leavese0
- Outcome
- endogenous BA levele0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The endogenous BA level of the infected cabbage leaves increased in response to Xcc infection.”
t0“Xanthomonas campestris”
Claim · model-written summary
BA significantly decreased Xcc virulence on cabbage.
- Taxon
- cabbageBrassica oleracea var. capitatae0
- Treatment
- BAe0
- Outcome
- virulence of Xcc on cabbagee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the presence of BA, the virulence of Xcc on cabbage decreased significantly.”
t0“Xanthomonas campestris”
The same questions in other papers
N-benzyladenine · molecular: expression · Brassica oleracea (wild cabbage)Positive and negative results
2 in the opposite direction
- positive
- positive
N-benzyladenine · disease · Brassica oleracea (wild cabbage)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.