Abstract
Yellow passion fruit (Passiflora edulis f. flavicarpa) is valuable edible specie with economic significance in China. Passion fruit faces yield losses due to viral diseases. This study used tissue culture of nodal shoot segments to propagate virus-free plants. Surface-sterilized nodal shoot segments were used and shoots were initiated on Murashige and Skoog (MS) medium supplemented with various concentrations of 6-Benzyladenine (6-BA) (0.0, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg L−1). Multiple shoots were initiated (5.2 ± 1.09 shoots/explant) with a 6-BA concentration of 2.0 mg L−1. Sub-culturing of these multiple shoots on MS medium augmented with 2.0 mg L−1 6-BA and 0.2 mg L−1 IAA was successful in vitro with maximum numbers of shoots (6.6 ± 1.17) within four weeks of incubation. These regenerated shoots were assayed for presence of passion fruit woodiness virus (PWV) using enzyme-linked immunosorbent assay (ELISA); only virus-free shoots were used for further propagation. Root formation was very good in each indole-3-butyric acid (IBA) and (IAA) at a concentration of 0.6 mg L−1 with a maximum number of roots (7.2 ±1.30). Rooted plantlets were gradually acclimatized in a greenhouse and successfully established in soil. Thus, virus-free plants can be successfully regenerated directly from nodal shoot segments.
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Claims 5
Claim · model-written summary
On MS medium with 6-BA at 2.0 mg L−1, nodal shoot segments of yellow passion fruit initiated multiple shoots (5.2 ± 1.09 shoots/explant).
- Taxon
- Yellow passion fruite0
- Treatment
- 6-BA concentration of 2.0 mg L−1e1
- Outcome
- Multiple shootse1
- Value
- 5.2 ± 1.09 shoots/explante1
Not reportedComparatorDirection
Verbatim
e0“Yellow passion fruit”
e1“Multiple shoots were initiated (5.2 ± 1.09 shoots/explant) with a 6-BA concentration of 2.0 mg L−1”
Claim · model-written summary
Sub-culturing on MS medium with 2.0 mg L−1 6-BA and 0.2 mg L−1 IAA gave the maximum number of shoots (6.6 ± 1.17) within four weeks.
- Taxon
- Yellow passion fruite0
- Treatment
- MS medium augmented with 2.0 mg L−1 6-BA and 0.2 mg L−1 IAAe1
- Outcome
- numbers of shootse1
- Direction
- maximume1
- Value
- 6.6 ± 1.17e1
Not reportedComparator
Verbatim
e0“Yellow passion fruit”
e1“MS medium augmented with 2.0 mg L−1 6-BA and 0.2 mg L−1 IAA was successful in vitro with maximum numbers of shoots (6.6 ± 1.17)”
Claim · model-written summary
Root formation was very good with IBA and IAA each at 0.6 mg L−1, with a maximum number of roots (7.2 ±1.30).
- Taxon
- Yellow passion fruite0
- Treatment
- indole-3-butyric acid (IBA) and (IAA) at a concentration of 0.6 mg L−1e1
- Outcome
- number of rootse1
- Direction
- maximume1
- Value
- 7.2 ±1.30e1
Not reportedComparator
Verbatim
e0“Yellow passion fruit”
e1“Root formation was very good in each indole-3-butyric acid (IBA) and (IAA) at a concentration of 0.6 mg L−1 with a maximum number of roots (7.2 ±1.30)”
Claim · model-written summary
Rooted plantlets were acclimatized in a greenhouse and successfully established in soil.
Not reportedDirection
Verbatim
e0“Yellow passion fruit”
e1“Rooted plantlets were gradually acclimatized in a greenhouse and successfully established in soil”
Claim · model-written summary
Virus-free plants can be regenerated directly from nodal shoot segments.
- Taxon
- Yellow passion fruite0
- Organ / stage
- nodal shoot segmentse1
- Outcome
- virus-free plants can be successfully regeneratede1
Not reportedDirection
Verbatim
e0“Yellow passion fruit”
e1“virus-free plants can be successfully regenerated directly from nodal shoot segments”
The same questions in other papers
indole-3-butyric acid · growth · Passiflora edulis (passion fruit)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.