Abstract
Root-knot nematodes (RKN; Meloidogyne species) are plant pathogens that introduce several effectors in their hosts to facilitate infection. The actual targets and functioning mechanism of these effectors largely remain unexplored. This study illuminates the role and interplay of the Meloidogyne javanica nematode effector ROS suppressor (Mj-NEROSs) within the host plant environment. Mj-NEROSs suppresses INF1-induced cell death as well as flg22-induced callose deposition and reactive oxygen species (ROS) production. A transcriptome analysis highlighted the downregulation of ROS-related genes upon Mj-NEROSs expression. NEROSs interacts with the plant Rieske's iron-sulfur protein (ISP) as shown by yeast-two-hybrid and bimolecular fluorescence complementation. Secreted from the subventral pharyngeal glands into giant cells, Mj-NEROSs localizes in the plastids where it interacts with ISP, subsequently altering electron transport rates and ROS production. Moreover, our results demonstrate that isp Arabidopsis thaliana mutants exhibit increased susceptibility to M. javanica, indicating ISP importance for plant immunity. The interaction of a nematode effector with a plastid protein highlights the possible role of root plastids in plant defense, prompting many questions on the details of this process.
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Claims 9
Claim · model-written summary
Mj-NEROSs suppresses INF1-induced cell death in plants.
- Taxon
- plantt0
- Treatment
- Mj-NEROSse1
- Interactor
- Meloidogyne javanicaMeloidogyne javanicae0pathogen
- Outcome
- INF1-induced cell deathe1
- Direction
- suppressese1
Not reportedComparator
Verbatim
t0“to suppress plant immunity”
e0“This study illuminates the role and interplay of the Meloidogyne javanica nematode effector ROS suppressor (Mj-NEROSs) within the host plant environment”
e1“Mj-NEROSs suppresses INF1-induced cell death as well as flg22-induced callose deposition and reactive oxygen species (ROS) production”
Claim · model-written summary
Mj-NEROSs suppresses flg22-induced callose deposition and reactive oxygen species production.
- Taxon
- plantt0
- Treatment
- Mj-NEROSse1
- Interactor
- Meloidogyne javanicaMeloidogyne javanicae0pathogen
- Outcome
- callose deposition and reactive oxygen species (ROS) productione1
- Direction
- suppressese1
- Qualifier
- flg22-inducede1
Not reportedComparator
Verbatim
t0“to suppress plant immunity”
e0“This study illuminates the role and interplay of the Meloidogyne javanica nematode effector ROS suppressor (Mj-NEROSs) within the host plant environment”
e1“Mj-NEROSs suppresses INF1-induced cell death as well as flg22-induced callose deposition and reactive oxygen species (ROS) production”
Claim · model-written summary
Mj-NEROSs expression downregulates ROS-related genes.
Not reportedComparator
Verbatim
t0“to suppress plant immunity”
e2“A transcriptome analysis highlighted the downregulation of ROS-related genes upon Mj-NEROSs expression”
Claim · model-written summary
Mj-NEROSs interacts with the plant Rieske iron-sulfur protein ISP, shown by yeast-two-hybrid and bimolecular fluorescence complementation.
Verbatim
t0“to suppress plant immunity”
e0“This study illuminates the role and interplay of the Meloidogyne javanica nematode effector ROS suppressor (Mj-NEROSs) within the host plant environment”
e3“NEROSs interacts with the plant Rieske's iron-sulfur protein (ISP) as shown by yeast-two-hybrid and bimolecular fluorescence complementation”
Claim · model-written summary
Mj-NEROSs localizes in the plastids after secretion into giant cells.
- Taxon
- plantt0
- Organ / stage
- plastidse4
- Interactor
- Meloidogyne javanicaMeloidogyne javanicae0pathogen
- Outcome
- localizes in the plastidse4
Verbatim
t0“to suppress plant immunity”
e0“This study illuminates the role and interplay of the Meloidogyne javanica nematode effector ROS suppressor (Mj-NEROSs) within the host plant environment”
e4“Secreted from the subventral pharyngeal glands into giant cells, Mj-NEROSs localizes in the plastids where it interacts with ISP, subsequently altering electron transport rates and ROS production”
Claim · model-written summary
Through its interaction with ISP, Mj-NEROSs alters electron transport rates and ROS production in plastids.
Not reportedDirection
Verbatim
t0“to suppress plant immunity”
e4“Secreted from the subventral pharyngeal glands into giant cells, Mj-NEROSs localizes in the plastids where it interacts with ISP, subsequently altering electron transport rates and ROS production”
Claim · model-written summary
Arabidopsis thaliana isp mutants show increased susceptibility to M. javanica.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae5 - Material
- isp Arabidopsis thaliana mutantse5
- Treatment
- M. javanicae5
- Interactor
- M. javanicaMeloidogyne javanicae5pathogen
- Outcome
- susceptibilitye5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Moreover, our results demonstrate that isp Arabidopsis thaliana mutants exhibit increased susceptibility to M. javanica, indicating ISP importance for plant immunity”
Claim · model-written summary
ISP is important for plant immunity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae5
- Gene
- ISPe5
- Outcome
- plant immunitye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Moreover, our results demonstrate that isp Arabidopsis thaliana mutants exhibit increased susceptibility to M. javanica, indicating ISP importance for plant immunity”
Claim · model-written summary
Root plastids may play a role in plant defense.
Verbatim
t0“to suppress plant immunity”
e6“The interaction of a nematode effector with a plastid protein highlights the possible role of root plastids in plant defense”
The same questions in other papers
pathogen product · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
2 in the opposite direction
- positive
- positive
pathogen product · cytology · Arabidopsis thaliana (thale cress)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.