Abstract
BACKGROUND: Rose (Rosa hybrida) is a globally recognized ornamental plant whose growth and distribution are strongly limited by drought stress. The role of Mediator, a multiprotein complex crucial for RNA polymerase II-driven transcription, has been elucidated in drought stress responses in plants. However, its physiological function and regulatory mechanism in horticultural crop species remain elusive. RESULTS: In this study, we identified a Tail module subunit of Mediator, RhMED15a-like, in rose. Drought stress, as well as treatment with methyl jasmonate (MeJA) and abscisic acid (ABA), significantly suppressed the transcript level of RhMED15a-like. Overexpressing RhMED15a-like markedly bolstered the osmotic stress tolerance of Arabidopsis, as evidenced by increased germination rate, root length, and fresh weight. In contrast, the silencing of RhMED15a-like through virus induced gene silencing in rose resulted in elevated malondialdehyde accumulation, exacerbated leaf wilting, reduced survival rate, and downregulated expression of drought-responsive genes during drought stress. Additionally, using RNA-seq, we identified 972 differentially expressed genes (DEGs) between tobacco rattle virus (TRV)-RhMED15a-like plants and TRV controls. Gene Ontology (GO) analysis revealed that some DEGs were predominantly associated with terms related to the oxidative stress response, such as 'response to reactive oxygen species' and 'peroxisome'. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment highlighted pathways related to 'plant hormone signal transduction', in which the majority of DEGs in the jasmonate (JA) and ABA signalling pathways were induced in TRV-RhMED15a-like plants. CONCLUSION: Our findings underscore the pivotal role of the Mediator subunit RhMED15a-like in the ability of rose to withstand drought stress, probably by controlling the transcript levels of drought-responsive genes and signalling pathway elements of stress-related hormones, providing a solid foundation for future research into the molecular mechanisms underlying drought tolerance in rose.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 13
Claim · model-written summary
identified a Tail module subunit of Mediator, RhMED15a-like, in rose
Verbatim
e0“identified a Tail module subunit of Mediator, RhMED15a-like, in rose”
t0“Rosa hybrida”
e1“identified a Tail module subunit of Mediator, RhMED15a-like, in rose”
e2“silencing of RhMED15a-like through virus induced gene silencing in rose”
Claim · model-written summary
Drought stress, as well as treatment with methyl jasmonate (MeJA) and abscisic acid (ABA), significantly suppressed the transcript level of RhMED15a-like
Not reportedComparator
Verbatim
e0“Drought stress, as well as treatment with methyl jasmonate (MeJA) and abscisic acid (ABA), significantly suppressed the transcript level of RhMED15a-like”
t0“Rosa hybrida”
e1“identified a Tail module subunit of Mediator, RhMED15a-like, in rose”
e2“silencing of RhMED15a-like through virus induced gene silencing in rose”
Claim · model-written summary
treatment with methyl jasmonate (MeJA) and abscisic acid (ABA), significantly suppressed the transcript level of RhMED15a-like
Not reportedComparator
Verbatim
e0“treatment with methyl jasmonate (MeJA) and abscisic acid (ABA), significantly suppressed the transcript level of RhMED15a-like”
t0“Rosa hybrida”
e1“identified a Tail module subunit of Mediator, RhMED15a-like, in rose”
e2“silencing of RhMED15a-like through virus induced gene silencing in rose”
Claim · model-written summary
treatment with methyl jasmonate (MeJA) and abscisic acid (ABA), significantly suppressed the transcript level of RhMED15a-like
Not reportedComparator
Verbatim
e0“treatment with methyl jasmonate (MeJA) and abscisic acid (ABA), significantly suppressed the transcript level of RhMED15a-like”
t0“Rosa hybrida”
e1“identified a Tail module subunit of Mediator, RhMED15a-like, in rose”
e2“silencing of RhMED15a-like through virus induced gene silencing in rose”
Claim · model-written summary
Overexpressing RhMED15a-like markedly bolstered the osmotic stress tolerance of Arabidopsis, as evidenced by increased germination rate, root length, and fresh weight
- Taxon
- Arabidopsis
Arabidopsise0e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpressing RhMED15a-like markedly bolstered the osmotic stress tolerance of Arabidopsis, as evidenced by increased germination rate, root length, and fresh weight”
e1“Overexpressing RhMED15a-like markedly bolstered the osmotic stress tolerance of Arabidopsis”
Claim · model-written summary
increased germination rate, root length, and fresh weight
- Taxon
- ArabidopsisArabidopsise0e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased germination rate, root length, and fresh weight”
e1“Overexpressing RhMED15a-like markedly bolstered the osmotic stress tolerance of Arabidopsis”
Claim · model-written summary
silencing of RhMED15a-like through virus induced gene silencing in rose resulted in elevated malondialdehyde accumulation
Not reportedComparator
Verbatim
e0“silencing of RhMED15a-like through virus induced gene silencing in rose resulted in elevated malondialdehyde accumulation”
t0“Rosa hybrida”
e1“identified a Tail module subunit of Mediator, RhMED15a-like, in rose”
e2“silencing of RhMED15a-like through virus induced gene silencing in rose”
Claim · model-written summary
silencing of RhMED15a-like through virus induced gene silencing in rose resulted in elevated malondialdehyde accumulation, exacerbated leaf wilting, reduced survival rate, and downregulated expression of drought-responsive genes during drought stress
Not reportedComparator
Verbatim
e0“silencing of RhMED15a-like through virus induced gene silencing in rose resulted in elevated malondialdehyde accumulation, exacerbated leaf wilting, reduced survival rate, and downregulated expression of drought-responsive genes during drought stress”
t0“Rosa hybrida”
e1“identified a Tail module subunit of Mediator, RhMED15a-like, in rose”
e2“silencing of RhMED15a-like through virus induced gene silencing in rose”
Claim · model-written summary
reduced survival rate
Not reportedComparator
Verbatim
e0“reduced survival rate”
t0“Rosa hybrida”
e1“identified a Tail module subunit of Mediator, RhMED15a-like, in rose”
e2“silencing of RhMED15a-like through virus induced gene silencing in rose”
Claim · model-written summary
downregulated expression of drought-responsive genes during drought stress
Not reportedComparator
Verbatim
e0“downregulated expression of drought-responsive genes during drought stress”
t0“Rosa hybrida”
e1“identified a Tail module subunit of Mediator, RhMED15a-like, in rose”
e2“silencing of RhMED15a-like through virus induced gene silencing in rose”
Claim · model-written summary
identified 972 differentially expressed genes (DEGs) between tobacco rattle virus (TRV)-RhMED15a-like plants and TRV controls
Verbatim
e0“identified 972 differentially expressed genes (DEGs) between tobacco rattle virus (TRV)-RhMED15a-like plants and TRV controls”
t0“Rosa hybrida”
e1“identified a Tail module subunit of Mediator, RhMED15a-like, in rose”
e2“silencing of RhMED15a-like through virus induced gene silencing in rose”
Claim · model-written summary
some DEGs were predominantly associated with terms related to the oxidative stress response, such as 'response to reactive oxygen species' and 'peroxisome'
Verbatim
e0“some DEGs were predominantly associated with terms related to the oxidative stress response, such as 'response to reactive oxygen species' and 'peroxisome'”
t0“Rosa hybrida”
e1“identified a Tail module subunit of Mediator, RhMED15a-like, in rose”
e2“silencing of RhMED15a-like through virus induced gene silencing in rose”
Claim · model-written summary
majority of DEGs in the jasmonate (JA) and ABA signalling pathways were induced in TRV-RhMED15a-like plants
Verbatim
e0“majority of DEGs in the jasmonate (JA) and ABA signalling pathways were induced in TRV-RhMED15a-like plants”
t0“Rosa hybrida”
e1“identified a Tail module subunit of Mediator, RhMED15a-like, in rose”
e2“silencing of RhMED15a-like through virus induced gene silencing in rose”
The same questions in other papers
drought · molecular: expression · RosaPositive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- no effect
loss of function · molecular: expression · RosaPositive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
1 paper more in this group
See all 5 papers →loss of function · disease · RosaPositive and negative results
5 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
4 papers more in this group
See all 8 papers →overexpression · growth · ArabidopsisPositive and negative results
70 papers in the same direction as this paper · 38 in the opposite direction
- negative
- negative
- negative
105 papers more in this group
See all 109 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.