Paper · 2023 · Al-Azhar Journal of Agricultural Research

Genetical studies on some genotypes in chickpea (Cicer arietinum L.) by Gamma Rays and ISSR Marker

Hussein Helal; Abd El Hadi Sayed; Ahmed Helal; et al.

8
claims
17
verbatim quotes
1
citations
3
research areas
✓
Open access

Abstract

The present investigation was conducted during the two seasons 2017-2018 and 2018-2019. Two field experiments were conducted with Egyptian soil cooperation between Genetic section, Agric-Botany Department, Faculty of Agriculture, Cairo, Al-Azhar University and Genetic Resources Research Department Bahteem, Field Crop Research Institute (FCRI), Agricultural Research Center (ARC), Bahteem Research Station, Egypt, on four chickpea genotypes (Cicer arietinum). The work was done to study genetic improvement of four chick pea genotypes (Cicer arietinum) using three different doses of gamma rays (200, 300 and 400 Gy) and Comparison of results through the morphological and molecular studies by ISSR-PCR primers. The results also indicate that the different doses of gamma rays (Except for the deadly ones) positively affected early rate with genotypesL1, L2, L3 and L4 compared to the control from each genotype, looking at the results of the second season. The doses of 400 affected the number of pods per plant and consequently on the number of seeds per plant in the genotype L4 by decrease it in the first season only, while it affected it by increase it in the second season. It also affected the number of seeds per pod by increase it in both seasons. It was also found that the ISSR technique was effective in this study, especially the primer ISSR-16, where the polymorphism rate was 95.7%, while the lowest percentage of Polymorphism was in the primer R-04, where the polymorphism rate was40%.

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Claims 8

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Gamma-ray doses positively affected early rate in chickpea genotypes L1-L4 compared with the control in the second season.

Taxon
Cicer arietinumCicer arietinume1
Material
L1, L2, L3 and L4e0
Treatment
different doses of gamma rayse0
Outcome
early ratee0
Direction
positively affectede0
Qualifier
looking at the results of the second seasone0
Comparator
controle0
Setting
Bahteem Research Station, Egypte1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the different doses of gamma rays (Except for the deadly ones) positively affected early rate with genotypesL1, L2, L3 and L4 compared to the control from each genotype, looking at the results of the second season”

✓ located verbatim in the source · abstract, characters 744–957 · show in text ↑
e1

“Bahteem Research Station, Egypt, on four chickpea genotypes (Cicer arietinum)”

✓ located verbatim in the source · abstract, characters 382–459 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The 400 Gy gamma-ray dose decreased the number of pods per plant in genotype L4 in the first season only.

Taxon
Cicer arietinumCicer arietinume1
Material
L4e2
Treatment
doses of 400e2
Outcome
number of pods per plante2
Direction
decreasee2
Qualifier
first season onlye2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e2

“The doses of 400 affected the number of pods per plant and consequently on the number of seeds per plant in the genotype L4 by decrease it in the first season only, while it affected it by increase it in the second season”

✓ located verbatim in the source · abstract, characters 959–1180 · show in text ↑
e1

“Bahteem Research Station, Egypt, on four chickpea genotypes (Cicer arietinum)”

✓ located verbatim in the source · abstract, characters 382–459 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The 400 Gy gamma-ray dose increased the number of pods per plant in genotype L4 in the second season.

Taxon
Cicer arietinumCicer arietinume1
Material
L4e2
Treatment
doses of 400e2
Outcome
number of pods per plante2
Direction
increasee2
Qualifier
in the second seasone2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e2

“The doses of 400 affected the number of pods per plant and consequently on the number of seeds per plant in the genotype L4 by decrease it in the first season only, while it affected it by increase it in the second season”

✓ located verbatim in the source · abstract, characters 959–1180 · show in text ↑
e1

“Bahteem Research Station, Egypt, on four chickpea genotypes (Cicer arietinum)”

✓ located verbatim in the source · abstract, characters 382–459 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The 400 Gy gamma-ray dose decreased the number of seeds per plant in genotype L4 in the first season only (inferred from the consequent effect on pods).

Taxon
Cicer arietinumCicer arietinume1
Material
L4e2
Treatment
doses of 400e2
Outcome
number of seeds per plante2
Direction
decreaseinferrede2
Qualifier
first season onlye2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e2

“The doses of 400 affected the number of pods per plant and consequently on the number of seeds per plant in the genotype L4 by decrease it in the first season only, while it affected it by increase it in the second season”

✓ located verbatim in the source · abstract, characters 959–1180 · show in text ↑
e1

“Bahteem Research Station, Egypt, on four chickpea genotypes (Cicer arietinum)”

✓ located verbatim in the source · abstract, characters 382–459 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The 400 Gy gamma-ray dose increased the number of seeds per plant in genotype L4 in the second season (inferred from the consequent effect on pods).

Taxon
Cicer arietinumCicer arietinume1
Material
L4e2
Treatment
doses of 400e2
Outcome
number of seeds per plante2
Direction
increaseinferrede2
Qualifier
in the second seasone2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e2

“The doses of 400 affected the number of pods per plant and consequently on the number of seeds per plant in the genotype L4 by decrease it in the first season only, while it affected it by increase it in the second season”

✓ located verbatim in the source · abstract, characters 959–1180 · show in text ↑
e1

“Bahteem Research Station, Egypt, on four chickpea genotypes (Cicer arietinum)”

✓ located verbatim in the source · abstract, characters 382–459 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Gamma-ray doses increased the number of seeds per pod in both seasons.

Taxon
Cicer arietinumCicer arietinume1
Treatment
different doses of gamma rayse4
Outcome
number of seeds per pode3
Direction
increasee3
Qualifier
in both seasonse3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e3

“It also affected the number of seeds per pod by increase it in both seasons”

✓ located verbatim in the source · abstract, characters 1182–1257 · show in text ↑
e4

“using three different doses of gamma rays (200, 300 and 400 Gy)”

✓ located verbatim in the source · abstract, characters 554–617 · show in text ↑
e1

“Bahteem Research Station, Egypt, on four chickpea genotypes (Cicer arietinum)”

✓ located verbatim in the source · abstract, characters 382–459 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The ISSR technique with primer ISSR-16 gave a polymorphism rate of 95.7% in chickpea genotypes.

Taxon
Cicer arietinumCicer arietinume1
Outcome
polymorphism ratee5
Value
95.7%e5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e5

“It was also found that the ISSR technique was effective in this study, especially the primer ISSR-16, where the polymorphism rate was 95.7%”

✓ located verbatim in the source · abstract, characters 1259–1398 · show in text ↑
e1

“Bahteem Research Station, Egypt, on four chickpea genotypes (Cicer arietinum)”

✓ located verbatim in the source · abstract, characters 382–459 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Primer R-04 gave the lowest polymorphism rate, 40%, among the ISSR primers tested in chickpea.

Taxon
Cicer arietinumCicer arietinume1
Outcome
polymorphism ratee6
Value
40%e6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e6

“the lowest percentage of Polymorphism was in the primer R-04, where the polymorphism rate was40%”

✓ located verbatim in the source · abstract, characters 1406–1502 · show in text ↑
e1

“Bahteem Research Station, Egypt, on four chickpea genotypes (Cicer arietinum)”

✓ located verbatim in the source · abstract, characters 382–459 · show in text ↑

radiation · yield · Cicer arietinum (chickpea)Results agree

6 papers: 6 positive, 0 negative or no effect

5 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.