Abstract
In vitro callus cultures of common chicory (Cichorium intybus L.) were obtained and their growth and biochemical characteristics depending on the hormonal composition of the MS medium and the spectral composition of light were studied. The study of the effect of light culture on callus tissue formation and inulin accumulation in it was carried out in opaque grow tents with radiation aligned with the flux density of pho- tosynthetic photons and different ratios of its levels in the region of 660 nm (R, red) and 730 nm (FR, far red). The control variant was placed under white linear fluorescent lamps. The resulting cultures were character- ized by high proliferative activity and the capability for morphogenesis. It has been established that the inter- action of two factors—the presence of auxins in the nutrient medium (IAA or NAA at a concentration of 7.5 mg/L in combination with BAP 0.5 mg/L) and cultivation under light culture conditions (FR R, FR = R, FR R)—had a significant impact on the biosynthetic potential of cell cultures. In the obtained cultures, a study of the quantitative content of inulin was carried out. It has been shown that the high content of inulin (7.55–7.95%) in callus cultures was on the MS medium in combination with IAA at FR R illumination. This is probably due to the fact that well proliferating and highly morphogenic callus tissue was formed under these conditions. The obtained results confirm the hypothesis about the specificity of cultured cells to in vitro synthesize and accumulate secondary metabolites in dedifferentiated cells and the dependence of this process on factors of chemical and physical nature.
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Claims 4
Claim · model-written summary
The obtained chicory callus cultures had high proliferative activity and morphogenic capacity.
Verbatim
e0“The resulting cultures were character- ized by high proliferative activity and the capability for morphogenesis”
e1“common chicory (Cichorium intybus L.)”
e2“callus cultures of common chicory”
Claim · model-written summary
Auxins and light conditions interacted to significantly affect biosynthetic potential of chicory cell cultures.
- Taxon
- common chicorye1
- Treatment
- presence of auxins in the nutrient mediume0
- Outcome
- biosynthetic potential of cell culturese0
- Qualifier
- cultivation under light culture conditionse0
Not reportedComparatorDirection
Verbatim
e0“presence of auxins in the nutrient medium (IAA or NAA at a concentration of 7.5 mg/L in combination with BAP 0.5 mg/L) and cultivation under light culture conditions (FR R, FR = R, FR R)—had a significant impact on the biosynthetic potential of cell cultures”
e1“common chicory (Cichorium intybus L.)”
Claim · model-written summary
Chicory callus on MS medium with IAA under FR R illumination contained 7.55–7.95% inulin.
- Taxon
- common chicorye1
- Organ / stage
- callus culturese0
- Treatment
- MS medium in combination with IAA at FR R illuminatione0
- Outcome
- content of inuline0
- Direction
- highe0
- Value
- 7.55–7.95%e0
Not reportedComparator
Verbatim
e0“high content of inulin (7.55–7.95%) in callus cultures was on the MS medium in combination with IAA at FR R illumination”
e1“common chicory (Cichorium intybus L.)”
Claim · model-written summary
The authors proposed that proliferative and morphogenic callus formation may explain the high inulin content.
Verbatim
e0“This is probably due to the fact that well proliferating and highly morphogenic callus tissue was formed under these conditions”
e1“high content of inulin (7.55–7.95%) in callus cultures”
e2“common chicory (Cichorium intybus L.)”
The same questions in other papers
indole-3-acetic acid · molecular: metabolites · Cichorium intybus (chicory)Results agree
1 paper in the same direction as this paper
- positive
light · molecular: metabolites · Cichorium intybus (chicory)Positive and negative results
1 in the opposite direction
- negative
in vitro · molecular: metabolites · Cichorium intybus (chicory)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.