Abstract
DUSPs, a diverse group of protein phosphatases, play a pivotal role in orchestrating cellular growth and development through intricate signaling pathways. Notably, they actively participate in the MAPK pathway, which governs crucial aspects of plant physiology, including growth regulation, disease resistance, pest resistance, and stress response. DUSP is a key enzyme, and it is the enzyme that limits the rate of cell metabolism. At present, complete understanding of the DUSP gene family in cotton and its specific roles in resistance to Verticillium wilt (VW) remains elusive. To address this knowledge gap, we conducted a comprehensive identification and analysis of four key cotton species: Gossypium arboreum, Gossypium barbadense, Gossypium hirsutum, and Gossypium raimondii. The results revealed the identification of a total of 120 DUSP genes in the four cotton varieties, which were categorized into six subgroups and randomly distributed at both ends of 26 chromosomes, predominantly localized within the nucleus. Our analysis demonstrated that closely related DUSP genes exhibited similarities in terms of the conserved motif composition and gene structure. A promoter analysis performed on the GhDUSP gene promoter revealed the presence of several cis-acting elements, which are associated with abiotic and biotic stress responses, as well as hormone signaling. A tissue expression pattern analysis demonstrated significant variations in GhDUSP gene expression under different stress conditions, with roots exhibiting the highest levels, followed by stems and leaves. In terms of tissue-specific detection, petals, leaves, stems, stamens, and receptacles exhibited higher expression levels of the GhDUSP gene. The gene expression analysis results for GhDUSPs under stress suggest that DUSP genes may have a crucial role in the cotton response to stress in cotton. Through Virus-Induced Gene Silencing (VIGS) experiments, the silencing of the target gene significantly reduced the resistance efficiency of disease-resistant varieties against Verticillium wilt (VW). Consequently, we conclude that GH_A11G3500-mediated bispecific phosphorylated genes may serve as key regulators in the resistance of G. hirsutum to Verticillium wilt (VW). This study presents a comprehensive structure designed to provide an in-depth understanding of the potential biological functions of cotton, providing a strong foundation for further research into molecular breeding and resistance to plant pathogens.
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Claims 11
Claim · model-written summary
A total of 120 DUSP genes were identified in four cotton species.
- Taxon
- cotton
Gossypium hirsutume0 - Outcome
- DUSP genese1
- Value
- 120e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we conducted a comprehensive identification and analysis of four key cotton species”
e1“The results revealed the identification of a total of 120 DUSP genes in the four cotton varieties”
Claim · model-written summary
The DUSP genes were categorised into six subgroups.
- Taxon
- cottonGossypium hirsutume0
- Outcome
- categorized into six subgroupse2
- Value
- six subgroupse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we conducted a comprehensive identification and analysis of four key cotton species”
e2“categorized into six subgroups”
Claim · model-written summary
The DUSP genes were randomly distributed at both ends of 26 chromosomes.
- Taxon
- cottonGossypium hirsutume0
- Outcome
- randomly distributed at both ends of 26 chromosomese3
- Value
- 26 chromosomese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we conducted a comprehensive identification and analysis of four key cotton species”
e3“randomly distributed at both ends of 26 chromosomes”
Claim · model-written summary
The DUSP proteins were predominantly localised within the nucleus.
- Taxon
- cottonGossypium hirsutume0
- Outcome
- predominantly localized within the nucleuse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we conducted a comprehensive identification and analysis of four key cotton species”
e4“predominantly localized within the nucleus”
Claim · model-written summary
Closely related DUSP genes showed similar conserved motif composition and gene structure.
- Taxon
- cottonGossypium hirsutume0
- Outcome
- conserved motif composition and gene structuree5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we conducted a comprehensive identification and analysis of four key cotton species”
e5“closely related DUSP genes exhibited similarities in terms of the conserved motif composition and gene structure”
Claim · model-written summary
The GhDUSP promoter contains cis-acting elements associated with abiotic and biotic stress responses and hormone signalling.
- Taxon
- cottonGossypium hirsutume0
- Gene
- GhDUSPe6
- Outcome
- cis-acting elementse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we conducted a comprehensive identification and analysis of four key cotton species”
e6“A promoter analysis performed on the GhDUSP gene promoter revealed the presence of several cis-acting elements, which are associated with abiotic and biotic stress responses, as well as hormone signaling”
Claim · model-written summary
GhDUSP expression under stress was highest in roots, followed by stems and leaves.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- rootse7
- Treatment
- different stress conditionse7
- Gene
- GhDUSPe7
- Outcome
- GhDUSP gene expressione7
- Direction
- higheste7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we conducted a comprehensive identification and analysis of four key cotton species”
e7“A tissue expression pattern analysis demonstrated significant variations in GhDUSP gene expression under different stress conditions, with roots exhibiting the highest levels, followed by stems and leaves”
Claim · model-written summary
GhDUSP expression was higher in petals, leaves, stems, stamens and receptacles.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- petals, leaves, stems, stamens, and receptaclese1
- Gene
- GhDUSPe1
- Outcome
- expression levels of the GhDUSP genee1
- Direction
- highere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we conducted a comprehensive identification and analysis of four key cotton species”
e1“petals, leaves, stems, stamens, and receptacles exhibited higher expression levels of the GhDUSP gene”
Claim · model-written summary
DUSP genes may have a crucial role in the cotton response to stress.
- Taxon
- cottonGossypium hirsutume0
- Gene
- DUSP genese1
- Outcome
- response to stresse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we conducted a comprehensive identification and analysis of four key cotton species”
e1“DUSP genes may have a crucial role in the cotton response to stress”
Claim · model-written summary
Silencing the target gene by VIGS significantly reduced resistance efficiency of disease-resistant cotton varieties against Verticillium wilt.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- Virus-Induced Gene Silencing (VIGS)e1
- Interactor
- VerticilliumVerticilliume1pathogen
- Gene
- GH_A11G3500e2
- Outcome
- resistance efficiencye1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we conducted a comprehensive identification and analysis of four key cotton species”
e1“Through Virus-Induced Gene Silencing (VIGS) experiments, the silencing of the target gene significantly reduced the resistance efficiency of disease-resistant varieties against Verticillium wilt (VW)”
e2“GH_A11G3500-mediated bispecific phosphorylated genes may serve as key regulators in the resistance of G. hirsutum to Verticillium wilt (VW)”
Claim · model-written summary
GH_A11G3500-mediated bispecific phosphorylated genes may be key regulators of Verticillium wilt resistance in G. hirsutum.
- Taxon
- G. hirsutumGossypium hirsutume1
- Gene
- GH_A11G3500-mediated bispecific phosphorylated genese1
- Outcome
- resistancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“GH_A11G3500-mediated bispecific phosphorylated genes may serve as key regulators in the resistance of G. hirsutum to Verticillium wilt (VW)”
The same questions in other papers
loss of function · Verticillium · Gossypium hirsutum (cotton)Positive and negative results
7 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
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See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.