Abstract
Phytophthora root rot is a devastating disease of soybean caused by Phytophthora sojae. However, the resistance mechanism is not yet clear. Our previous studies have shown that GmAP2 enhances sensitivity to P. sojae in soybean, and GmMYB78 is downregulated in the transcriptome analysis of GmAP2-overexpressing transgenic hairy roots. Here, GmMYB78 was significantly induced by P. sojae in susceptible soybean, and the overexpressing of GmMYB78 enhanced sensitivity to the pathogen, while silencing GmMYB78 enhances resistance to P. sojae, indicating that GmMYB78 is a negative regulator of P. sojae. Moreover, the jasmonic acid (JA) content and JA synthesis gene GmAOS1 was highly upregulated in GmMYB78-silencing roots and highly downregulated in overexpressing ones, suggesting that GmMYB78 could respond to P. sojae through the JA signaling pathway. Furthermore, the expression of several pathogenesis-related genes was significantly lower in GmMYB78-overexpressing roots and higher in GmMYB78-silencing ones. Additionally, we screened and identified the upstream regulator GmbHLH122 and downstream target gene GmbZIP25 of GmMYB78. GmbHLH122 was highly induced by P. sojae and could inhibit GmMYB78 expression in resistant soybean, and GmMYB78 was highly expressed to activate downstream target gene GmbZIP25 transcription in susceptible soybean. In conclusion, our data reveal that GmMYB78 triggers soybean sensitivity to P. sojae by inhibiting the JA signaling pathway and the expression of pathogenesis-related genes or through the effects of the GmbHLH122-GmMYB78-GmbZIP25 cascade pathway.
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Claims 14
Claim · model-written summary
P. sojae significantly induced GmMYB78 in susceptible soybean.
- Taxon
- soybean
Glycine maxe0 - Material
- susceptible soybeane0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- GmMYB78e0
- Outcome
- GmMYB78e0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmMYB78 was significantly induced by P. sojae in susceptible soybean”
Claim · model-written summary
GmMYB78 overexpression enhanced soybean sensitivity to the pathogen.
- Treatment
- overexpressing of GmMYB78e0
- Gene
- GmMYB78e0
- Outcome
- sensitivity to the pathogene0
- Direction
- enhancede0
Not reportedTaxonComparator
Verbatim
e0“the overexpressing of GmMYB78 enhanced sensitivity to the pathogen”
Claim · model-written summary
Silencing GmMYB78 enhanced resistance to P. sojae.
- Treatment
- silencing GmMYB78e0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- GmMYB78e0
- Outcome
- resistance to P. sojaee0
- Direction
- enhancese0
Not reportedTaxonComparator
Verbatim
e0“silencing GmMYB78 enhances resistance to P. sojae”
Claim · model-written summary
JA content was highly increased in GmMYB78-silencing roots.
- Organ / stage
- rootse0
- Treatment
- GmMYB78-silencinge0
- Outcome
- jasmonic acid (JA) contente0
- Direction
- highly upregulatede0
Not reportedTaxonComparator
Verbatim
e0“the jasmonic acid (JA) content and JA synthesis gene GmAOS1 was highly upregulated in GmMYB78-silencing roots”
Claim · model-written summary
GmAOS1 was highly upregulated in GmMYB78-silencing roots.
- Organ / stage
- rootse0
- Treatment
- GmMYB78-silencinge0
- Gene
- GmAOS1e0
- Outcome
- GmAOS1e0
- Direction
- highly upregulatede0
Not reportedTaxonComparator
Verbatim
e0“JA synthesis gene GmAOS1 was highly upregulated in GmMYB78-silencing roots”
Claim · model-written summary
JA content was highly downregulated in GmMYB78-overexpressing roots.
Not reportedTaxonComparator
Verbatim
e0“the jasmonic acid (JA) content and JA synthesis gene GmAOS1 was highly upregulated in GmMYB78-silencing roots and highly downregulated in overexpressing ones”
Claim · model-written summary
GmAOS1 was highly downregulated in GmMYB78-overexpressing roots.
Not reportedTaxonComparator
Verbatim
e0“JA synthesis gene GmAOS1 was highly upregulated in GmMYB78-silencing roots and highly downregulated in overexpressing ones”
Claim · model-written summary
Pathogenesis-related gene expression was significantly lower in GmMYB78-overexpressing roots.
- Organ / stage
- rootse0
- Treatment
- GmMYB78-overexpressinge0
- Outcome
- expression of several pathogenesis-related genese0
- Direction
- lowere0
Not reportedTaxonComparator
Verbatim
e0“the expression of several pathogenesis-related genes was significantly lower in GmMYB78-overexpressing roots”
Claim · model-written summary
Pathogenesis-related gene expression was higher in GmMYB78-silencing roots.
- Treatment
- GmMYB78-silencinge0
- Outcome
- expression of several pathogenesis-related genese0
- Direction
- highere0
Not reportedTaxonComparator
Verbatim
e0“the expression of several pathogenesis-related genes was significantly lower in GmMYB78-overexpressing roots and higher in GmMYB78-silencing ones”
Claim · model-written summary
GmbHLH122 was identified as an upstream regulator and GmbZIP25 as a downstream target of GmMYB78.
Not reportedTaxon
Verbatim
e0“we screened and identified the upstream regulator GmbHLH122 and downstream target gene GmbZIP25 of GmMYB78.”
Claim · model-written summary
P. sojae highly induced GmbHLH122 in resistant soybean.
- Taxon
- soybeanGlycine maxe0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- GmbHLH122e0
- Outcome
- GmbHLH122e0
- Direction
- highly inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmbHLH122 was highly induced by P. sojae and could inhibit GmMYB78 expression in resistant soybean”
Claim · model-written summary
GmbHLH122 could inhibit GmMYB78 expression in resistant soybean.
- Taxon
- soybeanGlycine maxe0
- Material
- resistant soybeane0
- Gene
- GmbHLH122e0
- Outcome
- GmMYB78 expressione0
- Direction
- inhibite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmbHLH122 was highly induced by P. sojae and could inhibit GmMYB78 expression in resistant soybean”
Claim · model-written summary
GmMYB78 activated GmbZIP25 transcription in susceptible soybean.
- Taxon
- soybeanGlycine maxe0
- Material
- susceptible soybeane0
- Gene
- GmMYB78e0
- Outcome
- GmbZIP25 transcriptione0
- Direction
- activatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmMYB78 was highly expressed to activate downstream target gene GmbZIP25 transcription in susceptible soybean”
Claim · model-written summary
The authors infer that GmMYB78 increases soybean sensitivity to P. sojae through suppression of JA signaling and pathogenesis-related genes or the GmbHLH122-GmMYB78-GmbZIP25 cascade.
- Taxon
- soybeanGlycine maxe0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- GmMYB78e0
- Outcome
- soybean sensitivity to P. sojaee0
- Qualifier
- JA signaling pathway and the expression of pathogenesis-related genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmMYB78 triggers soybean sensitivity to P. sojae by inhibiting the JA signaling pathway and the expression of pathogenesis-related genes or through the effects of the GmbHLH122-GmMYB78-GmbZIP25 cascade pathway.”
The same questions in other papers
loss of function · Phytophthora sojae · Glycine max (soybean)Positive and negative results
8 papers in the same direction as this paper · 15 in the opposite direction
- negative
- positive
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See all 24 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.