Abstract
The growth and yield of tea plants are seriously limited by drought stress. Fatty acid desaturations (FADs) contribute to mediating membrane fluidity in response to different stresses; however, the role of ω-3 FAD (Omega-3 fatty acid desaturase)-mediated damage induced by drought stress in tea plants is poorly understood. In this study, drought stress significantly promoted the synthesis of C18:3 (linolenic acid) and the expression level of CsFAD3. Yeast experiments further demonstrated that CsFAD3 can convert C18:2 to C18:3 and that the 35S:GFP-CsFAD3 fusion protein was localized in the endoplasmic reticulum of Nicotiana benthamiana (N. benthamiana) cells. CsFAD3-silenced tea leaves exhibited poor drought tolerance, with lower Fv/Fm and greater MDA (malondialdehyde) content compared with those of control plants. However, transgenic 35S:CsFAD3 Arabidopsis plants showed opposite phenotypes. In addition, JA (jasmonic acid) contents and the expression levels of CsLOX2, CsLOX4, CsAOS, CsAOC3 and CsOPR were significantly reduced in CsFAD3-silenced leaves under drought stress. However, no substantial difference in SA (salicylic acid) content was detected under normal or drought conditions. Analysis of Atcoi1 (JA receptor) or Atnpr1 (SA receptor) mutant Arabidopsis plants in 35S:CsFAD3 backgrounds further revealed that knockout of Atcoi1 impaired the drought-tolerant phenotypes of CsFAD3 overexpression lines. Therefore, our study demonstrated that CsFAD3 played a crucial role in drought tolerance by mediating JA pathways.
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Claims 13
Claim · model-written summary
Drought promoted C18:3 synthesis in tea.
Not reportedComparator
Verbatim
e0“drought stress significantly promoted the synthesis of C18:3 (linolenic acid) and the expression level of CsFAD3”
t0“tea”
Claim · model-written summary
Drought promoted CsFAD3 expression in tea.
- Taxon
- teat0
- Treatment
- drought stresse0
- Gene
- CsFAD3e0
- Outcome
- expression level of CsFAD3e0
- Direction
- promotede0
Not reportedComparator
Verbatim
e0“drought stress significantly promoted the synthesis of C18:3 (linolenic acid) and the expression level of CsFAD3”
t0“tea”
Claim · model-written summary
CsFAD3 converted C18:2 to C18:3 in yeast.
Verbatim
e0“CsFAD3 can convert C18:2 to C18:3”
t0“tea”
Claim · model-written summary
The CsFAD3 fusion protein localized in the endoplasmic reticulum of Nicotiana benthamiana cells.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Organ / stage
- cellse0
- Gene
- 35S:GFP-CsFAD3e0
- Outcome
- localized in the endoplasmic reticulume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“35S:GFP-CsFAD3 fusion protein was localized in the endoplasmic reticulum of Nicotiana benthamiana (N. benthamiana) cells”
Claim · model-written summary
CsFAD3 silencing reduced tea drought tolerance.
Not reportedComparator
Verbatim
e0“CsFAD3-silenced tea leaves exhibited poor drought tolerance”
Claim · model-written summary
CsFAD3 silencing lowered Fv/Fm under drought.
Verbatim
e0“CsFAD3-silenced tea leaves exhibited poor drought tolerance, with lower Fv/Fm and greater MDA (malondialdehyde) content compared with those of control plants”
Claim · model-written summary
CsFAD3 silencing raised MDA content under drought.
Verbatim
e0“CsFAD3-silenced tea leaves exhibited poor drought tolerance, with lower Fv/Fm and greater MDA (malondialdehyde) content compared with those of control plants”
Claim · model-written summary
CsFAD3 overexpression in Arabidopsis gave opposite drought phenotypes.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- 35S:CsFAD3e0
- Outcome
- opposite phenotypese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic 35S:CsFAD3 Arabidopsis plants showed opposite phenotypes”
Claim · model-written summary
JA content was reduced in silenced tea leaves under drought.
Not reportedComparator
Verbatim
e0“JA (jasmonic acid) contents and the expression levels of CsLOX2, CsLOX4, CsAOS, CsAOC3 and CsOPR were significantly reduced in CsFAD3-silenced leaves under drought stress”
t0“tea”
Claim · model-written summary
CsLOX2, CsLOX4, CsAOS, CsAOC3 and CsOPR expression fell in silenced tea leaves under drought.
- Taxon
- teat0
- Treatment
- CsFAD3-silencede0
- Gene
- CsLOX2, CsLOX4, CsAOS, CsAOC3 and CsOPRe0
- Outcome
- expression levels of CsLOX2, CsLOX4, CsAOS, CsAOC3 and CsOPRe0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“JA (jasmonic acid) contents and the expression levels of CsLOX2, CsLOX4, CsAOS, CsAOC3 and CsOPR were significantly reduced in CsFAD3-silenced leaves under drought stress”
t0“tea”
Claim · model-written summary
SA content did not substantially differ under normal or drought conditions.
Verbatim
e0“no substantial difference in SA (salicylic acid) content was detected under normal or drought conditions”
t0“tea”
Claim · model-written summary
Atcoi1 knockout impaired drought tolerance of CsFAD3 overexpression Arabidopsis lines.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- knockout of Atcoi1e0
- Gene
- Atcoi1e0
- Outcome
- drought-tolerant phenotypese0
- Direction
- impairede0
- Comparator
- CsFAD3 overexpression linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“knockout of Atcoi1 impaired the drought-tolerant phenotypes of CsFAD3 overexpression lines”
e1“mutant Arabidopsis plants in 35S:CsFAD3 backgrounds”
Claim · model-written summary
The authors conclude CsFAD3 mediates drought tolerance through JA pathways.
Verbatim
e0“CsFAD3 played a crucial role in drought tolerance by mediating JA pathways”
t0“tea”
The same questions in other papers
drought · physiology: hormones · Camellia sinensis (black tea)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.