Abstract
As part of our ongoing investigation of natural bioactive substances from the genus Thermopsis of the tribe Fabaceae for agricultural protection, the chemical constituents of the herb Thermopsis lupinoides were systematically investigated, which led to the isolation of 39 quinolizidine alkaloids (QAs) ( 1 – 39 ), including 14 new QAs ( 1 – 14 ) and 14 isoflavones ( 40 – 53 ). Their structures were elucidated through comprehensive spectroscopic data analysis (IR, UV, NMR, HRESIMS), ECD calculations, and X-ray crystallography. The antitomato spotted wilt virus (TSWV) and antifungal (against Botrytis cinerea, Gibberella zeae, Phytophythora capsica, and Alternaria alternata ) and insecticidal (against Aphis fabae and Tetranychus urticae ) activities of the isolated compounds were screened using the lesion counting method, mycelial inhibition assay, and spray method, respectively. The bioassay results showed that 34 exhibited excellent protective activity against TSWV, with an EC 50 value of 36.04 μg/mL, which was better than that of the positive control, ningnanmycin (86.03 μg/mL). The preliminary mechanistic exploration illustrated that 34 induced systemic acquired resistance in the host plant by acting on the salicylic acid signaling pathway. Moreover, 1 showed significant antifungal activity against B. cinerea (EC 50 value of 20.83 μg/mL), while 2 exhibited good insecticidal activity against A. fabae (LC 50 value of 24.97 μg/mL). This research is promising for the invention of novel pesticides from QAs with high efficiency and satisfactory ecological compatibility.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
The herb yielded 39 quinolizidine alkaloids, including 14 new ones.
- Taxon
- Thermopsis lupinoides
Thermopsis fabaceat0 - Outcome
- quinolizidine alkaloidse0
- Value
- 39e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolation of 39 quinolizidine alkaloids (QAs) ( 1 – 39 ), including 14 new QAs ( 1 – 14 )”
t0“Thermopsis lupinoides”
Claim · model-written summary
The herb yielded 14 isoflavones.
- Taxon
- Thermopsis lupinoidesThermopsis fabaceat0
- Outcome
- isoflavonese0
- Value
- 14e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“14 isoflavones ( 40 – 53 )”
t0“Thermopsis lupinoides”
Claim · model-written summary
Compound 34 protected against TSWV with EC50 36.04 μg/mL, better than ningnanmycin.
- Taxon
- Thermopsis lupinoidesThermopsis fabaceat0
- Treatment
- 34e0
- Interactor
- TSWV
Orthotospovirus tomatomaculaee0pathogen - Outcome
- protective activity against TSWVe0
- Direction
- bettere0
- Value
- 36.04 μg/mLe0
- Comparator
- ningnanmycine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“34 exhibited excellent protective activity against TSWV, with an EC 50 value of 36.04 μg/mL, which was better than that of the positive control, ningnanmycin (86.03 μg/mL)”
t0“Thermopsis lupinoides”
Claim · model-written summary
Compound 34 induced systemic acquired resistance through salicylic acid signaling.
- Taxon
- Thermopsis lupinoidesThermopsis fabaceat0
- Treatment
- 34e0
- Compound
- salicylic acide0
- Outcome
- systemic acquired resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“34 induced systemic acquired resistance in the host plant by acting on the salicylic acid signaling pathway”
t0“Thermopsis lupinoides”
Claim · model-written summary
Compound 1 inhibited B. cinerea with EC50 20.83 μg/mL.
- Taxon
- Thermopsis lupinoidesThermopsis fabaceat0
- Interactor
- B. cinerea
Botrytis cinereae0pathogen - Outcome
- antifungal activity against B. cinereae0
- Value
- 20.83 μg/mLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“1 showed significant antifungal activity against B. cinerea (EC 50 value of 20.83 μg/mL)”
t0“Thermopsis lupinoides”
Claim · model-written summary
Compound 2 had insecticidal activity against A. fabae with LC50 24.97 μg/mL.
- Taxon
- Thermopsis lupinoidesThermopsis fabaceat0
- Interactor
- A. fabae
Aphis fabaee0pest - Outcome
- insecticidal activity against A. fabaee0
- Value
- 24.97 μg/mLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2 exhibited good insecticidal activity against A. fabae (LC 50 value of 24.97 μg/mL)”
t0“Thermopsis lupinoides”