Abstract
Introduction The level of fatty acid unsaturation in seeds is one of the major determinants of cold germination ability, particularly in oilseeds. The presence of cis double bonds in unsaturated fatty acids creates bends that lowers their melting temperatures compared to saturated fatty acids. Unsaturated fatty acids with low melting points mobilize faster at low temperatures providing seeds with sufficient energy for germination. Methodology To investigate the effects of fatty acid unsaturation on the ability of cotton seeds to germinate under cold conditions, four recombinant inbred lines (RILs) of cotton with unique fatty acid profiles were evaluated using a set of developmental and biochemical assays at 12°C (critically low temperature), 15°C (cardinal minimum temperature) and 30°C (optimum temperature). Furthermore, whole seed lipidome profiling using liquid chromatography with mass spectrometry was done to compare the lipid compositional changes at 12°C and 30°C after imbibing cotton seeds of all the six genotypes for 0 hours, 3 hours and 6 hours. Results and discussion The RILs with higher unsaturation/saturation ratios registered robust germination performance, lower solute leakage, and optimum water uptake rates under cold stress. Imbibition at 30°C for 8 hours before cold exposure significantly improved the germination of cold sensitive genotypes, indicating that the first few hours of water uptake are critical for cold stress. Whole seed lipidome profiling of all the genotypes specifically associated cold germination ability with higher unsaturation levels of phospholipids during early imbibition. The presence of cis double bonds in phospholipids creates kinks that maintain the fluidity of cell membranes under low temperature. Membrane flexibility under cold conditions is essential for facilitating key germination events including membrane organization and respiration. The current results highlight the importance of fatty acid composition in cold germination ability of upland cotton.
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Claims 6
Claim · model-written summary
RILs with higher unsaturation ratios had robust cold germination.
- Taxon
- cotton
Gossypium hirsutumt0 - Material
- RILse0
- Compound
- unsaturation/saturation ratiose0
- Outcome
- germination performancee0
- Direction
- robuste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The RILs with higher unsaturation/saturation ratios registered robust germination performance”
t0“cotton”
Claim · model-written summary
RILs with higher unsaturation ratios had lower solute leakage.
- Taxon
- cottonGossypium hirsutumt0
- Material
- RILse0
- Compound
- unsaturation/saturation ratiose0
- Outcome
- solute leakagee0
- Direction
- lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The RILs with higher unsaturation/saturation ratios registered robust germination performance, lower solute leakage”
t0“cotton”
Claim · model-written summary
RILs with higher unsaturation ratios had optimum water uptake.
- Taxon
- cottonGossypium hirsutumt0
- Material
- RILse0
- Compound
- unsaturation/saturation ratiose0
- Outcome
- water uptake ratese0
- Direction
- optimume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The RILs with higher unsaturation/saturation ratios registered robust germination performance, lower solute leakage, and optimum water uptake rates under cold stress”
t0“cotton”
Claim · model-written summary
Warm imbibition before cold exposure improved germination of cold-sensitive genotypes.
- Taxon
- cottonGossypium hirsutumt0
- Material
- cold sensitive genotypese0
- Treatment
- Imbibition at 30°C for 8 hours before cold exposuree0
- Outcome
- germination of cold sensitive genotypese0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Imbibition at 30°C for 8 hours before cold exposure significantly improved the germination of cold sensitive genotypes”
t0“cotton”
Claim · model-written summary
Lipidome profiling associated cold germination ability with higher phospholipid unsaturation in early imbibition.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- early imbibitione0
- Compound
- phospholipidse0
- Outcome
- cold germination abilitye0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Whole seed lipidome profiling of all the genotypes specifically associated cold germination ability with higher unsaturation levels of phospholipids during early imbibition”
t0“cotton”
Claim · model-written summary
Cis double bonds in phospholipids were interpreted to maintain membrane fluidity at low temperature.
- Taxon
- cottonGossypium hirsutumt0
- Compound
- phospholipidse0
- Outcome
- fluidity of cell membranese0
- Qualifier
- cis double bondse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of cis double bonds in phospholipids creates kinks that maintain the fluidity of cell membranes under low temperature”
t0“cotton”
The same questions in other papers
cold · growth · Gossypium hirsutum (cotton)Results differ
2 papers in the same direction as this paper · 15 in the opposite direction
- negative
- positive
- negative
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